Using TruSeq DNA PCR-Free Sample Preparation Kit Library Kit (Illumina, San Diego, CA, USA) for library construction, the constructed library was quantified by Qubit and quantitative-PCR using the qualified library. Sequencing was performed using a HiSeq2500 PE250 (Illumina).
Truseq dna pcr free sample preparation kit library kit
Manufactured by Illumina
Sourced in United States
The TruSeq DNA PCR-Free Sample Preparation Kit Library Kit is a sample preparation kit designed for the generation of DNA libraries for next-generation sequencing. The kit utilizes a PCR-free library preparation method to avoid potential biases introduced by PCR amplification.
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Lab products found in correlation
2 protocols using truseq dna pcr free sample preparation kit library kit
1
Illumina TruSeq DNA PCR-Free Library Prep
2
Fecal Microbiome Profiling via 16S rRNA Sequencing
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Total RNA was extracted from 300 mg of feces, and the variable region V4 of the 16S gene was PCR-amplified using a fecal genome extraction kit. The primers used were: 515F (5- GTGCCAGCMGCCGCGGTAA-3′) and 805R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reaction was set up as follows: 94°C initial denaturation for 2 minutes; 94°C for 30 seconds, 72°C for 45 seconds for total 30 cycles; 72°C final extension for 5 minutes. Using the TruSeqDNA PCR-Free Sample Preparation Kit library kit (Illumina Company) for library construction, V4 bacterial rRNA genes were sequenced using the HiSeq2500PE250 platform.[8 (link)]
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