Immunoblots were performed using standard conditions as previously published [32 (link)]. The following antibodies were used in this study. Anti-phospho-Ser473-Akt (NEB 14543); anti-pan Akt (NEB 29020); anti-β-Actin (NEB 4967); anti-FLAG (Abgent AP1013a-ev). Anti-INPP4B antibody detecting murine Inpp4b was a kind gift from Jean Vacher [30 (link)].
Anti pan akt
Manufactured by New England Biolabs
Sourced in United Kingdom, Germany
Anti-pan Akt is a laboratory reagent that can be used to detect the presence and monitor the levels of the Akt protein, a key regulator of cell growth, proliferation, and survival. It is a primary antibody that specifically binds to the Akt protein, allowing for its identification and quantification in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by New England Biolabs (1 mentions)
Ptp1b, by Abcam (1 mentions)
Shp 1, by Abcam (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Anti phospho erk, by New England Biolabs (1 mentions)
Anti phospho akt ser473, by New England Biolabs (1 mentions)
Na934, by GE Healthcare (1 mentions)
Hrp linked anti rabbit, by GE Healthcare (1 mentions)
Anti phospho akt, by New England Biolabs (1 mentions)
3 protocols using anti pan akt
1
Immunoblotting Assay for Protein Phosphorylation
2
Phosphorylation Analysis of Insulin Receptor
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Preparation of protein lysates and immunoblotting was done by standard protocols with primary antibodies (anti‐phospho insulin receptor Y1158, anti‐phospho insulin receptor Y1361, anti‐phospho insulin receptor Y1162/63 (Abcam, Cambridge, UK), anti‐phospho Akt (Ser473), anti‐pan Akt, anti‐insulin receptor (4B8), anti‐phospho Erk, anti‐pan Erk (Cell Signaling/New England Biolabs, Frankfurt, Germany) and anti‐GAPDH (Millipore, Schwalbach, Germany). The recombinant proteins PTP1B and SHP‐1 (Abcam) were used for the dephosphorylation assay with phosphorylated insulin receptor as described 15 .
3
Protein Lysates and Wheat Germ Agglutinin Precipitation for DEP-1 Expression Analysis
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Preparation of protein lysates and wheat germ agglutinin precipitation (for DEP-1 expression analyses) were performed as described in Ref. [31] (link). Immunoblotting was done by standard protocols with primary antibodies: anti-phospho insulin receptor (IR) Y 972 (ab5678, 1:5000), anti-phospho IR Y 1158 (ab78355, 1:1000), anti-phospho IR Y 1361 (ab60946, 1:1000) (Abcam, Cambridge, UK), anti-DEP-1 (AF1934, 1:1000 of 1 μg/μl dilution, R&D Systems, Wiesbaden, Germany), anti-phospho Akt (#4060, 1:2000, Ser 473), anti-phospho Akt (#9275, 1:2000, Thr 308), anti-pan Akt (#9272, 1:1000) and anti-IR (#3025, 1:1000, 4B8) (Cell Signaling/New England Biolabs, Frankfurt, Germany). Secondary antibodies used were: HRP-linked anti-rabbit (NA934, 1:10,000, GE Healthcare), HRP-linked anti-goat (P 0160, 1:2000, Dako). Densitometric analyses were performed using ImageJ 1.46r.
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