Dehydrated alcohol for injection

Standard reagents and solvents were commercially available and used without further purification, unless otherwise noted: sodium chloride, 0.9% USP and sterile water for Injection, USP were purchased from Hospira; Dehydrated Alcohol for Injection, USP was obtained from Akorn Inc.; Ascorbic Acid for Injection, USP was acquired from Bioniche Pharma; molecular sieves were purchased from Alltech; and HPLC columns were acquired from Phenomenex. Other synthesis components were obtained as follows: sterile filters were acquired from Millipore; C18 Sep-Paks were purchased from Waters Corporation; 10 cc sterile vials were obtained from HollisterStier. Sep-Paks were flushed with 10 mL of ethanol followed by 10 mL of sterile water prior to use. [¹¹C]CO₂ was produced with a General Electric (GE) PETTrace cyclotron. High purity nitrogen containing 0.5% O₂ was irradiated with 16.5 MeV to generate [¹¹C]CO₂, which was delivered to a GE TRACERlab FX_C-Pro synthesis module via stainless steel lines. Radiochemical syntheses were carried out using GE TRACERLab FX_C-Pro synthesis modules housed in Comecer hot-cells. Doses produced were assessed via standard quality control techniques (see below) and were appropriate for rodent studies.

Reagents and solvents were commercially available and used without further purification, unless otherwise noted: sodium chloride (0.9% USP) and sterile water for Injection (USP) were purchased from Hospira; Dehydrated Alcohol for Injection (USP) was obtained from Akorn Inc. Shimalite-Nickle was purchased from Shimadzu; iodine was obtained from EMD; phosphorus pentoxide was acquired from Fluka; molecular sieves were purchased from Alltech; and HPLC columns were acquired from Phenomenex. Other synthesis components were obtained as follows: sterile filters were acquired from Millipore; C18-light Sep-Paks and Porapak Q were purchased from Waters Corporation; 10 cc sterile vials were obtained from HollisterStier. Sep-Paks were flushed with 10 mL of ethanol followed by 10 mL of sterile water prior to use.