Rabbit anti aciii

Both wholemounts and sections were incubated for 24–48 hours at 4°C with primary antibodies, followed by 24–48 hours at 4°C with the appropriate secondary a ntibodies (Molecular Probes, Invitrogen). The blocking solutions used were PBS/ 0.5% Triton X-100/ 10% Normal Donkey Serum (NDS) for wholemounts, and PBS / 0.1% Triton X-100/ 5% NDS for sections. For BrdU staining, samples were incubated for 40 min at 37°C w ith 2N HCl followed by 10 min at 25°C with 0.1 M boric acid (pH8.5), prior to incubation with antibodies. For mounting, the wholemounts and sections were embedded with Aqua Poly/Mount mounting medium (Polysciences). Primary antibodies: Mouse anti-AcTub (Sigma, 1:1000), Rabbit anti-5HT (Immunostar, 1:500), Rabbit anti-SERT (Immunostar, 1:500), Rabbit anti-synaptophysin (Abcam, 1:500), Goat anti-vGluT3 (Abcam, 1:200), Rabbit anti-ACIII (Santa Cruz, 1:500), Mouse anti-β-catenin (BD Biosciences, 1:500), Chicken anti-GFP (Aves Labs, 1:500), Rabbit anti-DCX (Cell Signaling, 1:200), Mouse anti-GFAP (Millipore, 1:1000), Mouse anti-Mash1 (BD Biosciences, 1:200) and rat anti-BrdU (Abcam, 1:500).