A computerized thrombelastography analyzer (TEG 5000, Haemonetics, Braintree, MA) was used to measure clot formation in the native FFP, MP-depleted FFP and MP-spiked FFP. The TEG measurements assessed included the initial clot formation time (R), clot formation rate (α-angle) and maximum clot strength (MA). Decreased R-time and increased α-angle and MA values indicated heightened coagulation potential. The reverse indicated decreased coagulation potential.
Analysis was performed according to the manufacturer’s instructions. Briefly, TEG sample cups containing 20 µL of 0.2 M calcium chloride (both from Haemonetics) were pre-warmed to 37°C by the TEG analyzer. The FFP sample (400 µL) was activated by incubation for 4 minutes at RT in a kaolin tube (Haemonetics), after which 340 µL of the kaolin-activated FFP were added to the TEG sample cup and analysis was initiated immediately. The reaction was terminated once the MA value was acquired.
Analysis was performed according to the manufacturer’s instructions. Briefly, TEG sample cups containing 20 µL of 0.2 M calcium chloride (both from Haemonetics) were pre-warmed to 37°C by the TEG analyzer. The FFP sample (400 µL) was activated by incubation for 4 minutes at RT in a kaolin tube (Haemonetics), after which 340 µL of the kaolin-activated FFP were added to the TEG sample cup and analysis was initiated immediately. The reaction was terminated once the MA value was acquired.