Anti tcrγ δ clone b1

Manufactured by BioLegend

Anti-TCRγ/δ(clone B1) is a monoclonal antibody that binds to the T cell receptor gamma/delta (TCRγ/δ) complex. This antibody can be used to identify and study T cells expressing the gamma/delta T cell receptor.

Automatically generated - may contain errors

Lab products found in correlation

Lsr 2 flow cytometer, by BD (1 mentions) Fcs express v3, by De Novo Software (1 mentions) Anti cd3 clone ucht1, by BioLegend (1 mentions) Anti tcr vβ11, by Beckman Coulter (1 mentions) Anti cd38 clone hit2, by BioLegend (1 mentions) Anti cd16 antibody clone 3g8, by BioLegend (1 mentions) Anti cd3 clone okt3, by BioLegend (1 mentions) Anti nkp30 clone p30 15, by BioLegend (1 mentions)

Close spelling variants of this product

TCRγ/δ (20 citations)

2 protocols using anti tcrγ δ clone b1

Comprehensive Immunophenotyping of iNKT Cells

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Data were acquired with a BD LSR II Flow Cytometer (BD Biosciences) and
analyzed with FCS Express V3 (De Novo Software, Los Angeles, CA). Doublets were
excluded with FSC-A and FSC-H linearity. Human antibodies were as follows:
anti-TCR Vα24-Jα18 (referred to as iNKT; clone 6B11), anti-CD3
(clone UCHT1), anti-CD4 (clone RPA-T4), anti-CD8 (clone RPA-T8), anti-CD34
(clone 561), anti-CD38 (clone HIT2, Biolegend) and anti-TCR
γ/δ(clone B1) from Biolegend, anti-TCR Vβ11 (Beckman
Coulter), anti-iNKT (BD Biosciences), CD1d tetramers loaded with PBS57, an
analog of α-GalCer (National Institutes of Health Tetramer Core
Facility, Atlanta, GA).

Sun W., Wang Y., East J.E., Kimball A.S., Tkaczuk K., Kesmodel S., Strome S.E, & Webb T.J. (2015). Invariant natural killer T cells generated from human adult hematopoietic stem-progenitor cells are poly-functional. Cytokine, 72(1), 48-57.

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Comprehensive Immune Checkpoint Assay

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The following reagents and antibodies were used: anti‐4‐1BB antibody (clone 4B4‐1, BioLegend, 309811; clone BBK‐2, LS‐C88297, LifeSpan BioSciences Inc; urelumab, produced internally), anti‐CD28 antibody (clone CD28.2, 16‐0289‐85; eBiosciences), anti‐NKp30 (clone P30‐15, 325204; BioLegend), anti‐NKp46 (clone 9E2, 331904; BioLegend), anti‐CD16 antibody (clone 3G8, 302050; BioLegend), anti‐CD20 (rituximab, hcd20‐mab1; Invivogen), anti‐CD3 (clone OKT3, 317326; BioLegend), anti‐TCR γδ (clone B1, 331236; BioLegend), MART‐1 (26‐35) peptide (EAAGIGILTV) (H‐4102.0005; Bachem), and NY‐ESO‐1 (157‐164‐V) peptide (SLLMWITQV; custom peptide synthesis, 96% pure; Bachem).

, & Vidard L. (2022). 4‐1BB and cytokines trigger human NK, γδ T, and CD8+ T cell proliferation and activation, but are not required for their effector functions. Immunity, Inflammation and Disease, 11(1), e749.

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