Synapt g2 si mass spectrometry

Acquity™ UPLC liquid chromatography (Waters, USA); Synapt™ G2-Si mass spectrometry (Waters, USA); Masslynx v4.1 workstation (Waters, USA); Ultrasonic cleaner (KQ-250 DB, Kunshan Ultrasonic Instrument Co., Ltd., China); Table centrifuge (sorvall ST 16R, Thermo Scientific, USA); Thermo Scientific 995 ultra low temperature refrigerator (Thermo Scientific, USA).

AA was bought from Sigma-Aldrich and was used without purification. LC-HRMS was performed on Waters Synapt G2Si Mass Spectrometry. The column model is ACQUITY UPLC BEH C18, 1.7 μm, 2.1 mm x 50 mm. Mobile phase A is 95% H₂O, 5% CAN, 0.1% FA, and mobile phase B is 5% H₂O, 95% CAN, 0.1% FA. The linear gradient sequence is 100% A, 0% B (0.5 min), 20% A, 80% B (4.0 min). 100% A, 0% B (4.1 min) with the flow rates at 0.3 mL/min. The auto sampler temperature was at room temperature. Negative mass spectrometry and electrospray ionization (ESI) method was used. 2000 V Voltage was used for ion spray, and the source temperature was 400 °C.

In this study, the instruments used included Acquity™ UPLC liquid chromatography (Waters, United States), Synapt™ G2-Si mass spectrometry (Waters, United States), Masslynx v4.1 workstation (Waters, United States), PRO-MRRM-8 small animal energy metabolism monitoring system (Sable Systems International, United States), KQ-250 DB ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., China), Sorvall ST 16R table centrifuge (Thermo Scientific, United States), and Thermo Scientific 995 ultra-low temperature refrigerator (Thermo Scientific, United States).