Cell lysates were diluted in Laemmli sample buffer containing β-mercaptoethanol and denatured at 100°C for 10 minutes. Samples were loaded on 4–15% mini-Protean tris/glycine gels (Biorad) and protein transferred to nitrocellulose. Membranes were blocked for 1 hour at room temperature in 5% milk then blotted overnight with one of the following primary antibodies: 1:500 rabbit anti-Smad1/5/8 (Cell Signaling), 1:500 rabbit anti-Smad1 (Cell Signaling), 1:500 rabbit anti-BMP4 (Abcam), or 1:500 rabbit anti-BMP2 (Abcam). After washes with 1x TBS/T, blots were incubated for 3 hours at room temperature with 1:3000 goat anti-rabbit secondary antibody (Cell Signaling). Blots were developed using SuperSignal West Pico Chemiluminescent substrate (Thermo Scientific) and exposed to film.
Rabbit anti bmp2
Manufactured by Abcam
Sourced in United Kingdom
Rabbit anti-BMP2 is a primary antibody that recognizes the bone morphogenetic protein 2 (BMP2) antigen. BMP2 is a member of the transforming growth factor-beta (TGF-beta) superfamily and plays a crucial role in bone and cartilage development. This antibody can be used for the detection and analysis of BMP2 in various applications, such as Western blotting, immunohistochemistry, and ELISA.
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Lab products found in correlation
Rabbit anti smad1, by Cell Signaling Technology (1 mentions)
Mini protean tris glycine gels, by Bio-Rad (1 mentions)
Rabbit anti smad 1 5 8, by Cell Signaling Technology (1 mentions)
Rabbit anti bmp4, by Abcam (1 mentions)
Goat anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions)
Anti runx2, by Abcam (1 mentions)
Rabbit anti col 1, by Abcam (1 mentions)
Sds page, by Solarbio (1 mentions)
Rabbit anti gapdh, by Bioworld Technology (1 mentions)
Mouse anti acetylatedα tubulin, by Abcam (1 mentions)
2 protocols using rabbit anti bmp2
1
Western Blot Analysis of BMP Signaling
2
Western Blot Analysis of Osteogenic Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total cellular proteins (20 μg from each sample) obtained above were separated by SDS-PAGE (Solarbio, Beijing, China) under reducing conditions and transferred onto a nitrocellulose membrane (Invitrogen, Shanghai, China). Membranes were blocked in 5% dry milk and probed overnight at 4 °C with gentle rocking with anti-Runx-2 (1:1000, Abcam, Cambridge, UK), rabbit anti-BMP-2 (1:1000, Abcam), rabbit anti-COL-1 (1:1000, Abcam), mouse anti-acetylated α-tubulin (1:1000, Abcam), rabbit anti-Dynlt1 (1:800, Abcam), rabbit anti-IFT88 (1:500, Santa Cruz Biotech, Dallas, Texas), or rabbit anti-GAPDH (1:800, Bioworld, Shanghai, China). After washes, membranes were incubated with a secondary antibody (HRP-conjugated goat anti-rabbit IgG or goat anti-mouse IgG, 1:10000, Bioworld). The proteins recognized by the antibody complexes were visualized by chemiluminescence (Solarbio, Beijing, China). The optical density of each maker band was measured using Image-Pro Plus 6.0 software.
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