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Anti human survivin antibody

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The Anti-human survivin antibody is a laboratory reagent used to detect and quantify the survivin protein in various samples. Survivin is a member of the inhibitor of apoptosis (IAP) protein family and plays a role in regulating cell division and preventing programmed cell death. The antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and flow cytometry to study the expression and localization of survivin in different cell types and tissues.

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Lab products found in correlation

Sds page gel, by Bio-Rad (2 mentions) Pvdf membrane, by GE Healthcare (2 mentions) Chemiluminescence reagent, by PerkinElmer (2 mentions) Anti gapdh, by Santa Cruz Biotechnology (2 mentions) Anti cugbp1, by Santa Cruz Biotechnology (2 mentions) Anti hur, by Santa Cruz Biotechnology (2 mentions) Image lab quantification software, by Bio-Rad (2 mentions)

Close spelling variants of this product

Survivin (21 citations) Anti-survivin (13 citations) Anti-survivin antibodies (6 citations) Survivin antibody (5 citations)

2 protocols using anti human survivin antibody

1

Western Blot Analysis of Protein Expression

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30 μg of protein from whole cell lysates was resolved on 10% SDS-PAGE gels (Bio-Rad Laboratories, Hercules, CA) and transferred onto PVDF membranes (GE Healthcare, Piscataway, NJ). After transfer, membranes were blocked in 5% nonfat milk in TBST and membranes were incubated with specific antibodies (overnight at 4°C) followed by horseradish peroxidaseconjugated anti-mouse or anti-rabbit (Santa Cruz, Dallas, TX) immunoglobulin for 1 hour at RT. Signal was detected by Chemiluminescence Reagent (PerkinElmer, Waltham, MA) and visualized by autoradiography. Anti-human survivin antibody was purchased from R&D Systems (Minneapolis, MN). Anti-CUG-BP1, anti-HuR, and anti-GAPDH antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX). All primary anti-bodies were used at a dilution of 1:2000 and all secondary antibodies were used at a dilution of 1:4000. Signal intensity was quantified using Image Lab quantification software (Bio-Rad, Hercules, CA).
Phatak P., Byrnes K.A., Mansour D., Liu L., Cao S., Li R., Rao J.N., Turner D.J., Wang J.Y, & Donahue J.M. (2015). Overexpression of miR-214-3p in Esophageal Squamous Cancer Cells Enhances Sensitivity to Cisplatin by Targeting Survivin Directly and Indirectly Through CUG-BP1. Oncogene, 35(16), 2087-2097.
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2

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
30 μg of protein from whole cell lysates was resolved on 10% SDS-PAGE gels (Bio-Rad Laboratories, Hercules, CA) and transferred onto PVDF membranes (GE Healthcare, Piscataway, NJ). After transfer, membranes were blocked in 5% nonfat milk in TBST and membranes were incubated with specific antibodies (overnight at 4°C) followed by horseradish peroxidaseconjugated anti-mouse or anti-rabbit (Santa Cruz, Dallas, TX) immunoglobulin for 1 hour at RT. Signal was detected by Chemiluminescence Reagent (PerkinElmer, Waltham, MA) and visualized by autoradiography. Anti-human survivin antibody was purchased from R&D Systems (Minneapolis, MN). Anti-CUG-BP1, anti-HuR, and anti-GAPDH antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX). All primary anti-bodies were used at a dilution of 1:2000 and all secondary antibodies were used at a dilution of 1:4000. Signal intensity was quantified using Image Lab quantification software (Bio-Rad, Hercules, CA).
Phatak P., Byrnes K.A., Mansour D., Liu L., Cao S., Li R., Rao J.N., Turner D.J., Wang J.Y, & Donahue J.M. (2015). Overexpression of miR-214-3p in Esophageal Squamous Cancer Cells Enhances Sensitivity to Cisplatin by Targeting Survivin Directly and Indirectly Through CUG-BP1. Oncogene, 35(16), 2087-2097.
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