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α cd103

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α-CD103 is a monoclonal antibody that binds to the CD103 antigen. CD103 is a type 1 transmembrane protein and an integrin subunit that is involved in lymphocyte trafficking and retention within epithelial tissues.

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Lab products found in correlation

Facs lysis buffer, by BD (2 mentions) α cd4, by BioLegend (2 mentions) α cd40, by BioLegend (2 mentions) Collagenase type 4, by Thermo Fisher Scientific (2 mentions) α cd44, by BD (2 mentions) α cd45, by BioLegend (2 mentions) α cd69, by BioLegend (2 mentions) α ifnar1, by BioLegend (2 mentions) Gentlemacs, by Merck Group (2 mentions) α cd8α, by BioLegend (2 mentions) Dnase 1, by Merck Group (2 mentions)

2 protocols using α cd103

1

Comprehensive Immune Cell Profiling of Mouse Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
At the designated times after infection, mice were euthanized, and tissues were perfused with PBS by cardiac injection. Lungs and/or dLN were harvested and single cell suspensions were created by enzymatic digestion with type IV collagenase (Fisher/Worthington) and DNase I (Sigma) and GentleMacs (Miltemyi) processing.
For flow cytometry staining, single cell homogenates were blocked in 2% rat serum for 10 min at room temperature, incubated with specified antibodies for 30 min on ice, fixed with BD FACS Lysis buffer for 10 min at room temperature, resuspended in PBS, and run on the LSRII (BD). Samples were analyzed using FlowJo software (BD).
The following anti-mouse antibodies conjugated to fluorochromes were utilized for flow cytometry: (company, clone) α-CD3ε (eBioscience/Invitrogen, 145–2C11), α-CD4 (Biolegend, GK1.5), α-CD8α (Biolegend, 53–6.7), α-CD11b (eBioscience/Invitrogen, M1/70), α-CD11c (eBioscience/Invitrogen, N418), α-CD19 (eBioscience/Invitrogen, 1D3), α-CD40 (Biolegend, HM40–3), α-CD44 (BD, IM7), α-CD45.1 (eBioscience/Invitrogen, A20), α-CD45.2 (Biolegend, 104), α-CD69 (Biolegend, H1.2F3), α-CD103 (Biolegend, 2E7), α-F4/80 (eBioscience/Invitrogen, BM8), α-IFNAR1 (Biolegend, MAR1–5A3), α-IFNγ (eBioscience/Invitrogen, XMG1.2), α-MHCII (eBioscience/Invitrogen, M5/114.15.2), α-TNFα (eBioscience/Invitrogen, MP6-XT22).
Hemann E.A., Green R., Turnbull J.B., Langlois R.A., Savan R, & Gale M J.r. (2019). Interferon-λ modulates dendritic cells to facilitate T cell immunity during infection with influenza A virus. Nature immunology, 20(8), 1035-1045.
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2

Comprehensive Immune Cell Profiling of Mouse Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
At the designated times after infection, mice were euthanized, and tissues were perfused with PBS by cardiac injection. Lungs and/or dLN were harvested and single cell suspensions were created by enzymatic digestion with type IV collagenase (Fisher/Worthington) and DNase I (Sigma) and GentleMacs (Miltemyi) processing.
For flow cytometry staining, single cell homogenates were blocked in 2% rat serum for 10 min at room temperature, incubated with specified antibodies for 30 min on ice, fixed with BD FACS Lysis buffer for 10 min at room temperature, resuspended in PBS, and run on the LSRII (BD). Samples were analyzed using FlowJo software (BD).
The following anti-mouse antibodies conjugated to fluorochromes were utilized for flow cytometry: (company, clone) α-CD3ε (eBioscience/Invitrogen, 145–2C11), α-CD4 (Biolegend, GK1.5), α-CD8α (Biolegend, 53–6.7), α-CD11b (eBioscience/Invitrogen, M1/70), α-CD11c (eBioscience/Invitrogen, N418), α-CD19 (eBioscience/Invitrogen, 1D3), α-CD40 (Biolegend, HM40–3), α-CD44 (BD, IM7), α-CD45.1 (eBioscience/Invitrogen, A20), α-CD45.2 (Biolegend, 104), α-CD69 (Biolegend, H1.2F3), α-CD103 (Biolegend, 2E7), α-F4/80 (eBioscience/Invitrogen, BM8), α-IFNAR1 (Biolegend, MAR1–5A3), α-IFNγ (eBioscience/Invitrogen, XMG1.2), α-MHCII (eBioscience/Invitrogen, M5/114.15.2), α-TNFα (eBioscience/Invitrogen, MP6-XT22).
Hemann E.A., Green R., Turnbull J.B., Langlois R.A., Savan R, & Gale M J.r. (2019). Interferon-λ modulates dendritic cells to facilitate T cell immunity during infection with influenza A virus. Nature immunology, 20(8), 1035-1045.
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