Dp74 color fluorescence camera

Cells were seeded onto coverslips in 24-well plate for growth to 70% cell confluence. Cells were fixed using 4% paraformaldehyde and blocked by 5% FBS and then subjected to incubation with the primary antibody for MOF or ERα (1:100) overnight at 4°C. After incubation with corresponding secondary antibody, cells were mounted with DAPI (4',6-diamidino-2-phenylindole) (C0060, Solarbio), and images were taken from a DP74 color fluorescence camera (Olympus, Tokyo, Japan)

2 × 10⁴ Cells were seeded in a 24-well plate containing cell culture slides and cultured overnight. Then the medium was replaced with a medium containing 100 μM CoCl₂ and cultured for 24 h. Fixed the cells with 1% formaldehyde for 15 min at room temperature, washed using PBS to remove the formaldehyde, used PBS containing 5‰ Tween-20 to incubate the slide for 20 min at room temperature. The slides were incubated for 1 h at room temperature with 10% FBS. The primary antibodies (1:200) were incubated overnight at 4°C, and the secondary antibodies (1:500) were incubated at room temperature for 2 h. DAPI (C0060, Solarbio) was used to indicate the nucleus. The DP74 color fluorescence camera (Olympus, Tokyo, Japan) was used to observe cell immunofluorescence.