Gapdh 14c10 number 2118

Rabbit pAb to human MxA (H-285) (ab-95926) was purchased from Abcam Inc. (Cambridge, MA, USA); Mouse mAb to the VSV nucleocapsid (N) designated 10G4 was a gift from Dr. Douglas S. Lyles (Wake Forest School of Medicine, Winston-Salem, NC, USA). Rabbit mAb to glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 14C10; number 2118) was obtained from Cell Signaling (Danvers, MA, USA), Anti-HA murine mAb (Cat. No. 100028-MM10) was purchased from Sino Biologicals US Inc. (Wayne, PA, USA). Respective AlexaFluor 488- and AlexaFluor 594-tagged secondary donkey antibodies to rabbit (A-11008 and A-11012) or mouse (A-21202 and A-21203) IgG were from Invitrogen Molecular Probes (Eugene, OR, USA). Alexafluor-594 tagged recombinant cholera toxin subunit B (CTB-red) was also purchased from Invitrogen Molecular Probes and used as per manufacturer’s recommendations (10 µM final concentration).

Rabbit pAb to human MxA (H-285) (ab-95926) was purchased from Abcam Inc. (Cambridge, MA, USA); Mouse mAb to the VSV nucleocapsid (N) designated 10G4 was a gift from Dr. Douglas S. Lyles (Wake Forest School of Medicine, NC, USA). Rabbit mAb to glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 14C10; number 2118) was obtained from Cell Signaling (Danvers, MA, USA). Respective AlexaFluor 488- and AlexaFluor 594-tagged secondary donkey antibodies to rabbit (A-11008 and A-11012) or mouse (A-21202 and A-21203) IgG were from Invitrogen Molecular Probes (Eugene, OR, USA).
Cyclosporin A (CsA) was purchased from APExBIO (Houston, TX, USA); calcien-AM, calyculin-A, okadaic acid, and tetraethylammonium chloride (TEA) (a “nonselective” K-channel blocker [31 (link)]) were purchased from Millipore-Sigma (St. Louis, MO, USA).
Lipton’s black tea, pre-ground Colombian coffee, and Propel were purchased from ShopRite supermarket (Elmsford, NY, USA). Tea was brewed by soaking one tea bag in one cup of boiling water (8 oz) for 2 min; coffee was brewed in a drip percolator (2 tablespoons coffee grounds per one cup of water). Drinking water was used from a water fountain in the laboratory. All beverages were equilibrated at 37 °C before use in cell culture experiments.