For animal modeling experiments, mice were randomly divided into three groups. Control group: Mice were fed a normal diet for 24 days, plus injection of DHEA solvent such as soybean oil into the back of the neck for the first 21 days. PCOS group: Mice were fed an HFD (High-fat diet) (consisting of 61.5% regular diet, 12% lard, 5% sucrose, 5% milk powder, 5% peanut, 10% egg, 1% sesame oil and 0.5% salt) and injected with DHEA (6 mg/kg/d, dissolved in DMSO and fused with soybean oil) on the back of neck for 21 days, followed by HFD for the remaining 3 days. Trehalose group: In addition to the intervention in the PCOS group, 2% Trehalose (R8530; Yuanye, China) were fed with water for 24 days (Trehalose dissolved in the drinking water of mice).
Trehalose
Manufactured by Yuanye Bio-Technology
Sourced in China
Trehalose is a natural disaccharide found in various organisms, including yeast, fungi, and insects. It functions as a stabilizer for proteins, enzymes, and cellular membranes, helping to maintain their structural integrity and activity. Trehalose can be used in a variety of lab applications where the preservation of biological materials is important.
Automatically generated - may contain errors
Lab products found in correlation
Curcumin, by Yuanye Bio-Technology (1 mentions)
Phosphatidylcholine, by Maclin Biochemical Technology (1 mentions)
α d lactose monohydrate, by Yuanye Bio-Technology (1 mentions)
Citric acid, by Yuanye Bio-Technology (1 mentions)
Fetal bovine serum (fbs), by Meilun (1 mentions)
Hydrogen peroxide h2o2, by Sinopharm (1 mentions)
Sodium dihydrogen phosphate, by Sinopharm (1 mentions)
Dibasic sodium phosphate, by Sinopharm (1 mentions)
Tunel cell apoptosis detection kit, by Transgene (1 mentions)
Anticleaved caspase 3antibody, by Affinity Biosciences (1 mentions)
Hrp conjugated goat anti rabbit igg secondary antibodies, by Affinity Biosciences (1 mentions)
Antibodies against gapdh, by Proteintech (1 mentions)
Annexin 5 fitc pi cell apoptosis detection kit, by Transgene (1 mentions)
Bcl 2, by Proteintech (1 mentions)
5 protocols using trehalose
1
PCOS Mouse Model with Trehalose
2
Trehalose Protects Aβ-Induced Fly Model
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Trehalose (Yuanye, Shanghai, China, # S11051) was dissolved and diluted to the final concentrations with ddH2O in this study. The administration method was completely based on a previous study [19 ]. Briefly, 80 μl of Trehalose solution with the final concentrations (0, 50, 100, and 200 mM) was daily added to the culture vial (with sufficient food) containing 20 newly eclosed subject flies (1–2 days old) until 25 days. The flies were transferred into fresh food every 7 days. The groups were designed as follows: wild-type group: A307 > W1118, Aβarc expression group: A307 > Aβarc, Aβarc expression plus low-dose Trehalose treatment group (TRE50): A307 > Aβarc + TRE 50 mM, Aβarc expression plus middle-dose Trehalose treatment group (TRE100): A307 > Aβarc + TRE 100 mM, and Aβarc expression plus high-dose Trehalose treatment group (TRE200): A307 > Aβarc + TRE 200 mM.
3
Trehalase Activity Determination Protocol
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Trehalase activity was determined using the 3,5-dinitrosalicylic acid method with modifications [22 (link)]. The reaction mixture consisted of 125 μL of extract of total proteins, 50 μL of 0.4 M trehalose (Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China), 125 μL of 0.2 M PBS (pH 6.0), 200 μL of validamycin (final concentrations of 0.5, 1, 2, 5, 10, and 20 μg/mL), and sterile water as control (CK). The mixture was incubated at 37 °C for 15 min, and trehalase activity was then determined. The reaction was stopped by adding 500 μL 3,5-dinitrosalicylic acid and boiled water for 5 min, and then the optical density at 540 nm (OD540) was measured after cooling. Trehalase activity was determined by measuring the content of glucose released during incubation. One unit of enzyme (U) was defined as the amount that hydrolyzes 1 μmol of trehalose per minute.
4
Optimizing Curcumin Delivery with Phospholipids
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PhosphatidylSerine (PS) was purchased from Chemi Nutra Inc. (Austin, TX, USA). Phosphatidylcholine (PC) was purchased from Shanghai Macklin Biochemical Co., Ltd. Curcumin, citric acid, α-D-Lactose monohydrate, trehalose, and mannitol were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). Sodium dihydrogen phosphate and dibasic sodium phosphate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Artificial saliva (CZ0243, pH6.8), simulated gastric fluid (CZ0212), and artificial intestinal juice (CZ0200) were purchased from Beijing Leagene Biotechnology Co., Ltd. (Beijing, China). Cell Counting Kit-8 (CCK-8), Dulbecco’s modified Eagle medium (DMEM) and fetal bovine serum (FBS) were obtained from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). Hydrogen peroxide (H2O2) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Reactive oxygen species (ROS) assay kit was purchased from Beijing Applygen Technology Co., Ltd. (Beijing, China). Ultra-pure water was obtained by MILLI-Q* ultra-pure water system.
5
Lipid-based Nanoparticle Formulation and Evaluation
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Raddeanin A (RA, purity≥98%), trehalose (purity≥98%), Rhodamine B (Rh, purity≥98%), HSPC (purity≥95%), and CHO (purity≥98%) were purchased from Yuanye Bio-Technology (Shanghai, China). DSPE-mPEG2k was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA). The Annexin V-FITC/PI cell apoptosis detection kit and TUNEL Cell Apoptosis Detection Kit were purchased from TransGen Biotech (Beijing, China). The MTT reagent kit, hematoxylin-eosin (H&E) Staining Kit, and Masson’s trichrome staining kit were purchased from Solarbio Science and Technology Company (Beijing, China). Antibodies against GAPDH (Cat# 10494-1-AP) and Bcl-2 (Cat# 26593-1-AP) were purchased from Proteintech (Wuhan, Hubei, China). Anti-cleaved-Caspase-3 antibody (Cat#AF7022), Ki67 (Cat#AF0198), and HRP-conjugated Goat Anti-Rabbit IgG secondary antibodies (Cat#S0001) were purchased from Affinity Bioscience (Changzhou, Jiangsu, China). Anti-HMGB1 (Cat#ab18256) and anti-AR (Cat#ab74272) antibodies were purchased from Abcam (Cambridge, MA, USA). The PSA Elisa Kit (Cat#MM-0469M1) was purchased from Jiangsu Meimian Industrial Co., Ltd. (Jiangsu, China).
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