Mueller hilton agar

Fourteen antibiotic disks (Oxoid, UK) infused with amplicin (10 μg), ampicillin/sulbactam (30 μg), amikacin (30 μg), cefotaxime (30 μg), ceftazidime (30 μg), chloramphenicol (30 μg), gentamicin (30 μg), imipenem (10 μg), kanamycin (30 μg), levofloxacin (5 μg), nalidixic acid (30 μg), oxytetracycline (30 μg), sulfamethoxazole/trimethoprim (25 μg), and tetracycline (30 μg) were used in this study. The antibiotic susceptibility of Vibrio spp. isolates were studied using the disk diffusion method (Yano et al., 2014 (link)). The antibiotic disks were dispensed on Mueller Hilton agar (HiMedia, India) supplemented with 2% w/v NaCl (Vivantis, USA) plates with bacterial lawn. After incubation at 37^∘C for 18 h, the inhibition zone is measured and interpreted guidelines of the Clinical and Laboratory Standard Institute (CLSI) (2010) M45-A2.

Antibiotic disks (Oxoid, UK) infused with 14 antibiotics namely ampicillin (10μg), ampicillin/sulbactam (30 μg), amikacin (30 μg), cefotaxime (30 μg), ceftazidime (30 μg), chloramphenicol (30 μg), gentamicin (30 μg), imipenem (10 μg), kanamycin (30 μg), levofloxacin (5 μg), nalidixic acid (30 μg), oxytetracycline (30 μg), Sulfamethoxazole/trimethoprim (25 μg), and tetracycline (30 μg) were used in this study. The antibiotic susceptibility of Vibrio sp. isolates were studied using the disk diffusion method (Yano et al., 2014 (link)). The Antibiotic disks were dispensed on Mueller Hilton agar (HiMedia, India) supplemented with 2% w/v NaCl (Vivantis, USA) plates with bacterial lawn. After incubation at 37°C for 18 h, the inhibition zone was measured and interpreted based on guidelines of the Clinical and Laboratory Standards Institute (CLSI) M45-A2 (Clinical Laboratory Standard Institute [CLSI], 2010 ).