HEK293T, MDAMB468, HCC1806 and HDQP1 were obtained from ATCC biobank. HEK293T and HDQP1 cells were cultured with DMEM supplemented with 10% foetal bovine serum (FBS), 1% L-glutamine, 1% penicillin/streptomycin (Euroclone, Milan, Italy), whereas MDAMB468 and HCC1806 cells were cultured with RPMI supplemented with 10% foetal bovine serum (FBS), 1% L-glutamine, 1% penicillin/streptomycin (Euroclone). Cells were kept at 37 °C under 5% CO2 atmosphere. SUM series cell lines were obtained from BioIVT biobank. SUM149PT, SUM185PE, SUM225CWN, SUM229PE and SUM159PT were cultured with Ham’s F12 medium (Gibco) supplemented with 5% heat-inactivated FBS (Gibco), 10 mM HEPES (Sigma-Aldrich), 1 μg/ml hydrocortisone (Sigma-Aldrich), 5 μg/ml insulin (Sigma-Aldrich) and 1% L-glutamine (Euroclone). SUM1315MO2 were cultured with the Ham’s F12 medium supplemented as described above with in addition 10 ng/mL EGF. No penicillin–streptomycin was added to the SUM cell line medium, as recommended by the suppliers.
L glutamine
Manufactured by Euroclone
Sourced in Italy, United States, United Kingdom, Germany, Israel, France, Switzerland
L-glutamine is an amino acid that plays a crucial role in various metabolic processes within the body. It serves as a building block for proteins and is involved in the production of other amino acids, nucleic acids, and energy-related compounds. This product is offered by Euroclone as a high-quality laboratory reagent for research and analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Euroclone (164 mentions)
Streptomycin, by Euroclone (162 mentions)
Penicillin, by Euroclone (157 mentions)
Penicillin streptomycin, by Euroclone (144 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (73 mentions)
Rpmi 1640 medium, by Euroclone (51 mentions)
Dulbecco modified eagle medium (dmem), by Euroclone (42 mentions)
Rpmi 1640, by Euroclone (38 mentions)
Fetal bovine serum (fbs), by Merck Group (24 mentions)
Dmem high glucose, by Euroclone (18 mentions)
Foetal bovine serum (fbs), by Euroclone (18 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (15 mentions)
Trypsin edta, by Euroclone (14 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (14 mentions)
Fetal calf serum (fcs), by Euroclone (12 mentions)
Non essential amino acids (neaa), by Euroclone (11 mentions)
Sodium pyruvate, by Euroclone (11 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (11 mentions)
Amphotericin b, by Euroclone (10 mentions)
L glutamine, by Merck Group (10 mentions)
502 protocols using l glutamine
1
Cell Culture Protocols for Cancer Lines
2
Cell Culture Conditions for Various Cell Lines
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MDCK cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Lonza) supplemented with 5% South American serum (EuroClone) and 2mM L-Glutamine (EuroClone). MDCK TetOFF cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Lonza) supplemented with 5% South American serum (EuroClone), 2mM L-Glutamine (EuroClone); controls were maintained in the presence of 1 μg/ml tetracycline (Sigma) to repress IRSp53 RNAi. Caco-2 cells were grown in Modified Eagle Medium (MEM, Biowest) supplemented with 20% South American serum, 2mM L-Glutamine and 0.1 mM NEAA. HEK 293-T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) supplemented with 10% South American serum (EuroClone) and 2mM L-Glutamine (EuroClone). Cells were grown at 37 °C in 5% CO2. HeLa cells were grown in Minimum Essential Medium (MEM Invitrogen) supplemented with 10% South American serum (EuroClone), 1% non-essential amino acids and 1% Sodium Pyruvate.
3
Culturing Cancer and Immune Cells
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The human prostate cancer (PCa) cell lines PC-3, DU-145, LNCaP (all purchased by ATCC) were maintained in in RPMI 1640 medium, supplemented with 10% Fetal Bovine Serum (FBS), (Euroclone), 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone), at 37°C, 5% CO2. Cells were routinely screened for eventual mycoplasma contaminations. Conditioned media (CM) were collected following 72 hours of starvation in FBS free RMPI 1640 (Life Technologies), supplemented with 1% Glutamine (Euroclone) and 1% P/S (Euroclone), at 37°C, 5% CO2. CMs were used for NK cell polarization as detailed below.
Human umbilical vein endothelial cells (HUVEC, Lonza) were maintained in endothelial cell basal medium (EBM™, Lonza) supplemented with endothelial cell growth medium (EGM™ SingleQuots™, Lonza), 10% of FBS, 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone). HUVEs were used between the 3-5 passages.
The human monocytic THP-1 cell line (ATCC) was cultured in RPMI 1640 medium, supplemented with 10% FBS, 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone), at 37°C, 5% CO2. Differentiation of adherent THP-1 macrophages was obtained following 48 hours of treatments with phorbol-merystate-acetate (5 ng/mL, PMA, Sigma Aldrich), as in [29] (link).
Human umbilical vein endothelial cells (HUVEC, Lonza) were maintained in endothelial cell basal medium (EBM™, Lonza) supplemented with endothelial cell growth medium (EGM™ SingleQuots™, Lonza), 10% of FBS, 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone). HUVEs were used between the 3-5 passages.
The human monocytic THP-1 cell line (ATCC) was cultured in RPMI 1640 medium, supplemented with 10% FBS, 2 mM l-Glutamine (Euroclone), 100 U/mL penicillin and 100 μg/mL streptomycin (Euroclone), at 37°C, 5% CO2. Differentiation of adherent THP-1 macrophages was obtained following 48 hours of treatments with phorbol-merystate-acetate (5 ng/mL, PMA, Sigma Aldrich), as in [29] (link).
4
Cell Line Authentication and Propagation
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Cell lines were tested and authenticated following the manufacturer’s instructions: DSMZ for NB4 and ATCC for all the others. HCT-116 and HT-29 (colon cancer), MCF7 (breast cancer), A549 (lung cancer), MiaPaCa (pancreas carcinoma), and A2058 (melanoma) cells were propagated in Dulbecco’s modified Eagle’s medium (Euroclone, Milan, Italy) with 10% fetal bovine serum (FBS) (Euroclone), 2 mM L -glutamine (Euroclone) and antibiotics (100 U/ml penicillin, 100 mg/ml streptomycin; Euroclone). NB4 (acute promyelocytic leukemia) and K562 (chronic myelogenous leukemia) cells were propagated in RPMI-1640 medium containing 4.5 g/L glucose (Euroclone) supplemented with 10% FBS (Euroclone), 100 U/mL penicillin–streptomycin (Euroclone) and 2 mM L -glutamine (Euroclone). MRC5 (normal human lung) cells were propagated in Eagle’s minimum essential medium (Euroclone) supplemented with 10% FBS (Euroclone), and 10 μg/ml gentamicin solution (Euroclone).
5
Culturing SKNBE2 and SHSY5Y Neuroblastoma Cells
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SKNBE2 and SHSY5Y neuroblastoma cells were provided by the cell bank of the National Institute of Cancer Research (IST) Genoa, Italy and obtained from ECACC.
SKNBE2 were maintained in RPMI 1640 medium (EuroClone, Devon, UK), 10% FBS (GIBCO, S. Giuliano Milanese, Milan, Italy), 2 mM L-glutamine (EuroClone), 100 U/mL penicillin–streptomycin (EuroClone). SHSY5Y cells were cultured in Dulbecco’s modified Eagles medium (DMEM) (EuroClone), 10% FBS (GIBCO), 2 mM L-glutamine (EuroClone), and 100 U/mL penicillin-streptomycin (EuroClone).
SKNBE2 were maintained in RPMI 1640 medium (EuroClone, Devon, UK), 10% FBS (GIBCO, S. Giuliano Milanese, Milan, Italy), 2 mM L-glutamine (EuroClone), 100 U/mL penicillin–streptomycin (EuroClone). SHSY5Y cells were cultured in Dulbecco’s modified Eagles medium (DMEM) (EuroClone), 10% FBS (GIBCO), 2 mM L-glutamine (EuroClone), and 100 U/mL penicillin-streptomycin (EuroClone).
6
Culturing LNCaP and U937 Cell Lines
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Cell culture LNCaP and U937 were purchased from ATCC (Milan, Italy). LNCaP were grown in Roswell Park Memorial Institute culture medium (RPMI; EuroClone, Milan, Italy), supplemented with 10% heat-inactivated foetal bovine serum (FBS; Sigma-Aldrich, Schnellendorf, Germany), antimicrobials (100 U/mL penicillin, 100 µg/mL streptomycin, 250 ng/mL amphotericin-B), 2 mM L-glutamine (EuroClone, Milan, Italy), and 1% essential amino acids solution (MEM; EuroClone, Milan, Italy). U937 were cultured in Dulbecco’s Modified Eagle Medium (DMEM; EuroClone, Milan, Italy), supplemented with 10% heat-inactivated foetal bovine serum (FBS; Sigma-Aldrich, Schnellendorf, Germany), antimicrobials (100 U/mL penicillin, 100 µg/mL streptomycin, 250 ng/mL amphotericin-B), 2 mM L-glutamine (EuroClone). Cells have grown in standard incubator conditions at 37 °C and 5% CO2.
7
Fabrication and Characterization of PBS Biomaterials
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Poly (1,4-butylene succinate) extended with 1,6-diisocyanatohexane (Tm 120 °C) (PBS), 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) and Dulbecco’s phosphate buffered saline (DPBS) were purchased from Aldrich Milan, Italy. This merchant specifies only the Tm for PBS, but Fabbri et al. performed a GPC analysis to evaluate the molecular weight (81.2 kDa) [21 (link)].
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), trypsin, l-glutamine, penicillin, streptomycin, and amphotericin were purchased from Euroclone group (Milan, Italy).
Porcine bile was extracted from pigs at Istituto Zooprofilattico della Sicilia “A. Mirri,” Palermo, Italy accordingly with European rules on animal experiments.
Human blood was extracted from volunteers upon informed consent and isolated at the University of Palermo, Palermo, Italy.
NHDF-Ad-Human Dermal Fibroblasts, Adult were obtained from Lonza bioscience and used after 9 doublings. The cell line was grown in a minimum essential medium [Dulbecco’s modified Eagle’s medium (DMEM)] supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM l-glutamine, 100 um/mL penicillin, 100 μg/mL streptomycin, and 2.5 μg/mL amphotericin B (all reagents were from Euroclone, Milan, Italy) under standard conditions (95% relative humidity, 5% CO2, 37 °C).
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), trypsin, l-glutamine, penicillin, streptomycin, and amphotericin were purchased from Euroclone group (Milan, Italy).
Porcine bile was extracted from pigs at Istituto Zooprofilattico della Sicilia “A. Mirri,” Palermo, Italy accordingly with European rules on animal experiments.
Human blood was extracted from volunteers upon informed consent and isolated at the University of Palermo, Palermo, Italy.
NHDF-Ad-Human Dermal Fibroblasts, Adult were obtained from Lonza bioscience and used after 9 doublings. The cell line was grown in a minimum essential medium [Dulbecco’s modified Eagle’s medium (DMEM)] supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mM l-glutamine, 100 um/mL penicillin, 100 μg/mL streptomycin, and 2.5 μg/mL amphotericin B (all reagents were from Euroclone, Milan, Italy) under standard conditions (95% relative humidity, 5% CO2, 37 °C).
8
Cervical Cancer Cell Line Culture
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SiHa cell line, isolated from squamous cell carcinoma and containing HPV-16 genome (1–2 copies per cell), was obtained from ATCC® (ATCC® HTB35™). SiHa cells were cultured in DMEM medium (Euroclone, Pero, Italy) supplemented with 10% FBS (Fetal bovine serum), 1% L-glutamine, 1% penicillin and streptomycin (Euroclone, Pero, Italy).
CaSki cell line, originally isolated from a cervical carcinoma and containing 600 copies of integrated HPV-16, was obtained from BEI-Resource. CaSki cells were cultured in RPMI-1640 medium (Euroclone, Pero, Italy) supplemented with 10% FBS (Fetal bovine serum), 1% L-glutamine, 1% penicillin and streptomycin (Euroclone, Pero, Italy).
CaSki cell line, originally isolated from a cervical carcinoma and containing 600 copies of integrated HPV-16, was obtained from BEI-Resource. CaSki cells were cultured in RPMI-1640 medium (Euroclone, Pero, Italy) supplemented with 10% FBS (Fetal bovine serum), 1% L-glutamine, 1% penicillin and streptomycin (Euroclone, Pero, Italy).
9
Neuroblastoma Cell Culture Protocols
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SKNBE2 and SHSY5Y neuroblastoma cells were provided by the cell bank of the National Institute of Cancer Research (IST) Genoa, Italy and obtained from ECACC. SKNBE2 (KCB Cat# KCB 201199YJ, RRID:CVCL_0528) were cultured in RPMI 1640 medium (EUROCLONE S.p.A. Pero, Milan, Italy), supplemented with 10% FBS (EUROCLONE S.p.A. Pero, Milan, Italy), L-glutamine (2 mM; EUROCLONE S.p.A. Pero, Milan, Italy), and penicillin-streptomycin (100 U/mL/100 ug/mL; EUROCLONE S.p.A. Pero, Milan, Italy). SHSY5Y (KCB Cat# KCB 2006107YJ, RRID:CVCL_0019) cells were cultured in Dulbecco’s modified Eagles medium (DMEM) (EUROCLONE S.p.A. Pero, Milan, Italy), 10% FBS (EUROCLONE S.p.A. Pero, Milan, Italy), 2 mM L-glutamine (EUROCLONE S.p.A. Pero, Milan, Italy), and 100 U/mL penicillin-streptomycin (EUROCLONE S.p.A. Pero, Milan, Italy). Cultures were maintained at 37 °C in a humidified 5% CO2 atmosphere.
10
Culturing Thyroid Cancer Cell Lines
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Three human thyroid cancer cell lines FTC-133 (Sigma-Aldrich, Italy), K1 (Sigma-Aldrich, Italy), and 8505c (Sigma-Aldrich, Italy) were used in this study. FTC-133 cells were cultured in DMEM: Ham’s F12 (1:1) (Sigma-Aldrich, Italy) supplemented with L-Glutamine 2 mM (Euroclone, Italy), penicillin/streptomycin/amphotericin (PSA) (Euroclone, Italy), and 10% Fetal Bovine Serum (FBS) (Sigma-Aldrich, Italy). K1 cells were cultured in DMEM: Ham’s F12: MCDB 105 (2:1:1) (Sigma-Aldrich, Italy) supplemented with L-Glutamine 2 mM (Euroclone, Italy), penicillin/streptomycin/amphotericin (PSA) (Euroclone, Italy), and 10% Fetal Bovine Serum (FBS) (Sigma-Aldrich, Italy), while for 8505c cells, the medium employed was EMEM (Sigma-Aldrich, Italy) containing 1% of nonessential amino acids, L-Glutamine 2 mM, PSA, and 10% FBS. Cells were maintained in a humidified environment at 37°C and 5% CO2/95% air atmosphere and cultured in 75 cm2 culture flasks. The medium was replaced twice a week and cells were split at about 80–90% of the confluence.
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