Hp 5ms column

Manufactured by Agilent Technologies

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The HP-5MS column is a fused silica capillary column used for gas chromatography. It is designed for the separation and analysis of a wide range of organic compounds.

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HP-5MS (342 citations) HP-5MS fused silica capillary column (56 citations) HP-5MS Ultra Inert capillary column (12 citations) HP-5MS GC column (11 citations) HP-5MS fused silica column (9 citations) HP-5MS quartz capillary column (9 citations) J&W HP-5ms GC Column (8 citations) 30 m HP-5ms column (8 citations) Agilent HP-5MS capillary column (6 citations) J&W HP-5MS capillary column (6 citations)

378 protocols using hp 5ms column

GC and GC-MS Analysis of Compounds

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Gas chromatographic (GC) analysis was performed on an Agilent Technologies HP 7890A Plus Gas chromatograph equipped with a FID and fitted with HP-5ms column (30 m × 0.25 mm, film thickness 0.25 μm, Agilent Technologies, Santa Clara, CA, USA). The analytical conditions were: carrier gas H₂ (1 mL/min), injector temperature (PTV: programmable temperature vaporization) 250 °C, detector temperature 260 °C, column temperature programmed from 60 °C (2 min hold) to 220 °C (10 min hold) at 4 °C/min. Samples were injected using a split mode with a split ratio of 10:1. The volume injected was 1.0 μL. Inlet pressure was 6.1 kPa.
An Agilent Technologies (Santa Clara, CA, USA) HP 7890A Plus Chromatograph fitted with a fused silica capillary HP-5ms column (30 m × 0.25 mm, film thickness 0.25 μm) and interfaced with a mass spectrometer HP 5973 MSD was used for the GC/MS analysis, under the same conditions as those used for GC analysis. The conditions were the same as described above with He (1 mL/min) as carrier gas. The MS conditions were as follows: ionization voltage 70 eV; emission current 40 mA; acquisitions scan mass range of 35–350 amu at a sampling rate of 1.0 scan/s. Compound identification was carried out by comparison of the MS fragmentation patterns and calculated retention indices with those available in the databases [35 ,36 ,37 ] and, when available, with standard substances.

Chau D.T., Chung N.T., Huong L.T., Hung N.H., Ogunwande I.A., Dai D.N, & Setzer W.N. (2020). Chemical Compositions, Mosquito Larvicidal and Antimicrobial Activities of Leaf Essential Oils of Eleven Species of Lauraceae from Vietnam. Plants, 9(5), 606.

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GC and GC-MS Analysis of Essential Oils

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Gas chromatography (GC) analysis was performed on an Agilent Technologies HP 6890 Plus Gas chromatograph equipped with a FID and fitted with HP-5MS column (30 m x 0.25 mm, film thickness 0.25 m, Agilent Technology). The analytical conditions were: carrier gas H2 (1 mL/min), injector temperature (PTV) 250ºC, detector temperature 260ºC, column temperature programmed from 60ºC (2 min hold) to 220ºC (10 min hold) at 4 o C/min. Samples were injected by splitting and the split ratio was 10:1. The volume injected was 1.0 L. Inlet pressure was 6.1 kPa. Each analysis was performed in triplicate. The relative amounts of individual components were calculated based on the GC peak area (FID response).
An Agilent Technologies HP 6890N Plus Chromatograph fitted with a fused silica capillary HP-5 MS column (30 m x 0.25 mm, film thickness 0.25 m) and interfaced with a mass spectrometer HP 5973 MSD was used for the GC/MS analysis, under the same conditions as those used for GC analysis. The conditions were the same as described above with He (1 mL/min) as a carrier gas. The MS conditions were as follows: ionization voltage 70 eV; emission current 40 mA; acquisitions scan mass range of 35-350 amu at a sampling rate of 1.0 scan/s. The MS fragmentation patterns were checked with those of other essential oils of known composition.

Huong L.T., Huong T.T., Huong N.T., Hung N.H., Dat P.T.T., Luong N.X., & Ogunwande I.A. (2020). Chemical composition and larvicidal activity of essential oils from Zingiber montanum (J. Koenig) Link ex. A. Dietr. against three mosquito vectors. Boletin Latinoamericano y del Caribe de plantas Medicinales y Aromaticas, 19(6), 569-579.

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GC and GC/MS Analysis of Samples

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Gas chromatography (GC) analysis was performed on an Agilent Technologies HP 6890 Plus Gas chromatograph equipped with a FID and fitted with HP-5MS column (30 m ×0.25 mm, film thickness 0.25 µm, Agilent Technology) . The analytical conditions were: carrier gas He (1 mL/min) , injector temperature (PTV) 250℃, detector temperature 260℃, column temperature programmed from 60℃ (2 min hold) to 220℃ (10 min hold) at 4 ℃/min. Samples were injected by splitting and the split ratio was 10:1. The volume injected was 1.0 µL. Inlet pressure was 6.1 kPa. The relative amounts of individual components were calculated based on the GC peak area (FID response) , as previously described 17, 18) .
An Agilent Technologies HP 6890N Plus Chromatograph fitted with a fused silica capillary HP-5 MS column (30 m× 0.25 mm, film thickness 0.25 µm) and interfaced with a mass spectrometer HP 5973 MSD was used for the gas chromatography-mass spectrometry (GC/MS analysis) The same conditions described above was also used for GC. The mass spectrum operating conditions were as follows: ionization voltage 70eV; emission current 40 mA; acquisitions scan mass range of 35-350 amu at a sampling rate of 1.0 scan/s.

Huong L.T., Huong T.T., Huong N.T.T., Hung N.H., Dat P.T.T., Luong N.X, & Ogunwande I.A. (2020). Mosquito Larvicidal Activity of the Essential Oil of Zingiber collinsii against Aedes albopictus and Culex quinquefasciatus. Journal of oleo science, 69(2).

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Metabolomic Analysis of Glycolysis, Citric Acid Cycle and Amino Acids

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We analyzed the concentrations of metabolites involved in glycolysis, citric acid cycle and amino acid metabolism using gas chromatography coupled to quadrupole time-of-flight mass spectrometry with an electron impact source (GC-EI-QTOF-MS), as we have described previously [12 (link)]. Analyses were performed with a 7890A gas chromatograph coupled, with an electron impact source, to a 7200 quadrupole time-of-flight mass spectrometer equipped with a 7693 autosampler module and a J&W Scientific HP-5MS column (J&W Scientific HP-5MS column, 30 m × 0.25 mm, 0.25 μm, Agilent Technologies, Santa Clara, CA, USA). Calibration curves were obtained for each metabolite by plotting standard concentrations as a function of the peak area. Recovery of each metabolite was calculated, and ranged between 83% and 99%.

Arenas M., Rodríguez E., García-Heredia A., Fernández-Arroyo S., Sabater S., Robaina R., Gascón M., Rodríguez-Pla M., Cabré N., Luciano-Mateo F., Hernández-Aguilera A., Fort-Gallifa I., Camps J, & Joven J. (2018). Metabolite normalization with local radiotherapy following breast tumor resection. PLoS ONE, 13(11), e0207474.

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Chemical Profiling of Massoia and Nutmeg Oils

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Agilent 7890B GC and an Agilent 5977B MSD mass spectrometer were used to identify the chemical components of massoia and nutmeg EOs. An HP-5MS column (length: 30 m; internal diameter: 0.25 mm; film thickness: 0.25 μm) (J&W Scientific, Folsom, CA, USA) was used, and the flow rate of the carrier gas (helium) was 1.0 mL/min. The oven was programmed to be isothermal at 40 °C for 5 min then, heat to 250 °C at the rate of 6 °C/min and hold at this temperature for 5 min. Ionization was obtained by electron impact (70 eV, source temperature of 230 °C), and the scan range was 41 − 400 amu. Most EO components were identified by comparing the mass spectra of each peak with those of standard compounds obtained from the National Institute of Standards and Technology mass spectrometry library.

Seo S.M., Lee J.W., Shin J., Tak J.H., Hyun J, & Park I.K. (2021). Development of cellulose nanocrystal-stabilized Pickering emulsions of massoia and nutmeg essential oils for the control of Aedes albopictus. Scientific Reports, 11, 12038.

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GC-MS Analysis of Volatile Compounds

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An Agilent 6890/5975C GC‐MS system equipped with HP‐5 ms column (30 m × 0.25 mm i.d., 0.25 μm film thickness; J&W Scientific) was used to analyze volatile compounds that accumulated on the SPME fiber. The carrier gas was helium with splitless mode, which was delivered at a linear velocity of 1 ml/min. The desorption time was 5 min in the injection port at 250°C. The temperature was programmed to be hold at 35°C for 3 min and increased to 280°C at a rate of 5°C/min. The mass selective detector was operated in the electron impact ionization mode at 70 eV, in the scan range m/z 40–400. The interface temperature was 230°C, and the retention time of each volatile was converted to the Kovats retention index using n‐alkanes (Sigma, Co.) as references. The volatile compounds were tentatively identified by matching the mass spectra with the spectra of the reference compounds in both the Wiley mass spectra (MS) library (8th edn) and the NIST/EPA/NIH MS library (version 2.2a) and verified on the basis of mass spectra obtained from the literature and comparison of Kovats retention indices with those reported in the literature. Finally, identification accuracy was determined by separating relevant standard compounds through GC‐MS analysis under the same conditions (Dong et al., 2013).

Xiao L., Li C., Chai D., Chen Y., Wang Z., Xu X., Wang Y., Geng Y, & Dong L. (2020). Volatile compound profiling from soybean oil in the heating process. Food Science & Nutrition, 8(2), 1139-1149.

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GC-MS Analysis of Volatile Compounds

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The CrBL-EO treatments were analyzed to investigate the volatile compounds using an Agilent GC–MS system equipped with a gas chromatograph (7890A) and a mass spectrometer detector (5975C). The GC was fitted with an HP-5MS column (30 m × 0.25 mm internal diameter and 0.25 µm film thickness, J&W Scientific, Folsom, CA, USA) to separate the volatile compounds. Helium served as a carrier gas at a 1 mL/min flow rate with a 10:1 split ratio and a splitless injection volume of 1 µL. The gradient temperature was programmed as follows: 40 °C for 1 min, rising to 150 °C at 4 °C/min and maintained for 6 min; rising at 4 °C/min to 210 °C and maintained for 1 min. The injector and the detector were kept at 280 °C and 220 °C, respectively. For mass spectrometry (MS), electron ionization (EI) with an ionization energy voltage of 70 eV, emission current of 35 mA, and a spectral zone of 40–550 m/z was performed. The National Institute of Standards and Technology (NIST) 08, 2005 Library (Gaithersburg, MD, USA), Wiley 7 Library (Wiley, New York, NY, USA), and published mass spectra were used to identify the essential oil components based on their MS data.

Rashed M.M., You L., Ghaleb A.D, & Du Y. (2022). Two-Phase Extraction Processes, Physicochemical Characteristics, and Autoxidation Inhibition of the Essential Oil Nanoemulsion of Citrus reticulata Blanco (Tangerine) Leaves. Foods, 12(1), 57.

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Volatile Compound Extraction from (E)-4-Decenal

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Extraction and collection of volatile compounds from (E)-4-decenal during heating was performed by using a thermal desorption cryo-trapping system, with a qualified microchamber/thermal extractor (M−CTE250 Markes International, UK). (E)-4-decenal was diluted to 100 μL/mL, 20 μL of the sample was taken and 10 μL of diluted cyclohexanone (2 μg/mL) was added as a quantitative internal standard. An Agilent 6890/5975C GC–MS system (Santa Clara, CA, USA) equipped with an HP-5 ms column (30 m × 0.25 mm i.d., 0.25 μm film thickness; J&W Scientific) was used for the analysis of volatile compounds desorbed from the sample (Chai, Li, & Zhang, 2019 (link), Drabova et al., 2013 (link), Dymerski, 2017 (link), Jeleń et al., 2012 (link), Peter, Giorgia, & Mariarosa, 2016 (link)). Specific parameters from the experiments are detailed in the Supplementary Material.

Wang B., Dou S., Wang S., Wang Y., Zhang S., Lin X., Chen Y., Ji C., Dai Y, & Dong L. (2024). Mechanism of thermal oxidation into volatile compounds from (E)-4-decenal: A density functional theory study. Food Chemistry: X, 21, 101174.

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Fatty Acid Profiling of Insect Nymphs

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The fat bodies dissected from ten 2nd–3rd instar nymphs were frozen in liquid nitrogen and ground in 1 mL of a 2% H₂SO₄/98% methanol solution (5 μL 200 ug/mL C19:0 as internal reference). Samples were sealed with a cap and incubated at 80 °C for 1 h. After the addition of 0.3 ml of hexane and 1.5 ml of H₂O, the fatty acid methyl esters were extracted into the hexane layer by shaking and then were centrifuged at 5000× g for 10 min. Samples of the organic phase were carried out by means of GC-MS on an Agilent Technologies 6890N GC-5973N mass selective detector. The GC was equipped with a HP-5MS column (60 mm × 0.25 mm; film thickness 0.25 μm) (J&W Scientific, Folsom, CA, USA) and carried out following the procedure described previously [47 (link)]. Compounds were identified by comparing their retention time with those of authentic reference compounds and comparing the spectra with that of the mass spectral library NIST02 (Rev. D.04.00; Agilent Technologies, Palo Alto, CA, USA).

Zhou X., Ling X., Guo H., Zhu-Salzman K., Ge F, & Sun Y. (2021). Serratia symbiotica Enhances Fatty Acid Metabolism of Pea Aphid to Promote Host Development. International Journal of Molecular Sciences, 22(11), 5951.

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Terpenoid Profiling by GC-MS

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Terpenoid detection was analyzed using an Agilent 7890A gas chromatograph coupled to an Agilent 5975C Network Mass Selective Detector (MS, insert XL MSD with triple-axis detector). Chromatographic column: HP-5MS column (30 m × 0.25 mm × 0.25 μm; J&W Scientific, Folsom, CA, USA); Carrier gas: He (purity ≥ 99.999%); Injection port temperature: 280 °C; Injection method: Splitless injection; Injection volume: 1 µL. Program temperature rise: the initial temperature is 60 °C for 2 min, the temperature is increased at 20 °C/min to 220 °C for 1 min, the temperature is increased at 5 °C.min⁻¹ to 250 °C for 1 min, and finally the temperature is increased at 20 °C.min⁻¹ to 290 °C for 7.5 min.
Ion source: EI; ion source temperature: 230 °C; ion energy mode: use tuning setting; ion energy (eV): 70; detector setting: use gain factor; solvent delay: 5 min; mass scan range: 30~500.

Song C., Guan Y., Zhang D., Tang X, & Chang Y. (2022). Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV–B-Controlled Terpenoid Synthesis in Fragrant Woodfern (Dryopteris fragrans). International Journal of Molecular Sciences, 23(10), 5708.

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