Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti c myc

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Rabbit anti-c-Myc is a primary antibody produced in rabbit that recognizes the c-Myc protein. It is commonly used in various research applications such as Western blotting, immunoprecipitation, and immunohistochemistry to detect and study the expression of the c-Myc protein.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti cyclin d1, by Cell Signaling Technology (3 mentions) Mouse anti β actin, by Merck Group (3 mentions) Rabbit anti β catenin, by Cell Signaling Technology (2 mentions) Rabbit anti erk1 2, by Cell Signaling Technology (2 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (2 mentions) Ab54759, by Merck Group (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Gradient gel, by Thermo Fisher Scientific (2 mentions) β actin, by Merck Group (2 mentions) Pvdf membrane, by Abcam (2 mentions) Peroxidase conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) X omat film, by Kodak (2 mentions) Enhanced chemi luminescence (ecl), by Cytiva (2 mentions) Mouse anti γh2ax, by Merck Group (2 mentions) Goat anti rrm2, by Santa Cruz Biotechnology (2 mentions) Mouse anti cyclin a, by Leica Biosystems (2 mentions) Mouse anti brdu fitc, by BD (2 mentions) Rabbit anti phospho erk1 2, by Cell Signaling Technology (2 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Rabbit anti gapdh, by Cell Signaling Technology (2 mentions)

28 protocols using rabbit anti c myc

1

Isolation and Characterization of Testicular Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Testicular single-cell suspensions were prepared from 2–3-month-old Col1a1-4F2A-OSKM mice as described previously [34 ]. The immunomagnetic selection of α6+ cells was performed using anti-α6 integrin-PE (GoH3) (BD Pharmingen) and anti-PE microbeads (Miltenyi Biotec) according to the manufacturer's protocol. Hoechst staining (5 μg/ml) of the cell suspensions was performed as described previously [34 ]. Cells were then labelled with β2m-FITC (Santa Cruz) and anti-CD117-APC (2B8) antibodies (BD Pharmingen). Propidium iodide (Sigma) was added before cell sorting to exclude dead cells. To analyse the transcription factors, cells were fixed and permeabilized using a Cytofix/Cytoperm kit (BD Pharmingen) and stained with the following antibodies: Alexa Fluor 488 mouse anti-Nanog (BD Pharmingen 560261 clone M55-312), Alexa Fluor 647 mouse anti-Sox2 (BD Pharmingen 560294 Clone 245610), rabbit anti-c-Myc (Cell Signaling), rabbit anti-Kfl4 (Abcam), rabbit anti-Lin28 (Cell Signaling), rabbit IgG isotype control (Cell Signaling) and secondary anti-rabbit Alexa Fluor 488 antibody along with APC-anti-PLZF antibody (R&D). Analyses and cell sorting were performed on ARIA, LSR II and FACSCalibur flow cytometers (Becton Dickinson).
Corbineau S., Lassalle B., Givelet M., Souissi-Sarahoui I., Firlej V., Romeo P.H., Allemand I., Riou L, & Fouchet P. (2016). Spermatogonial stem cells and progenitors are refractory to reprogramming to pluripotency by the transcription factors Oct3/4, c-Myc, Sox2 and Klf4. Oncotarget, 8(6), 10050-10063.
+ Open protocol
+ Expand
2

Gastric Cancer Cell Line Characterization

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HGC-27 and AGS gastric cancer cells were purchased from Shanghai Cell Bank of Chinese Academy of Sciences, MKN45 and GES-1 were gifts from Digestive Tumor Research Institute of Fujian Medical University. HGC-27, GES-1 and MKN45 cells were cultured in RPMI 1640 medium with 10% fetal bovine serum (Cat.11875–093, Cat.10099–141, Gibco,Thermo Fisher Scientific, Shanghai, China) and 1% Penicillin/Streptomycin Solution (100×, Gibco, Thermo Fisher Scientific, Shanghai, China). The total protein lysates were blotted with following antibodies: mouse anti-Pin1 (1:3000) from professor Lu of Harvard Medical University27 (link). Mouse anti-Actin (1:3000,#HC201–02; TransGen Biotech, Beijing, China).The following antibodies were purchased from Cell Signaling Technology: rabbit anti-Cyclin D1 (#2978,1:1000), rabbit anti-Phospho-Akt(Ser473) (#9271, 1:1000); rabbit anti-β-Catenin(#8480, 1:1000); rabbit anti-Phospho-GSK3beta(Ser9)(#9336,1:1000); rabbit anti-c-Myc (#9402, 1:1000), rabbit anti-CyclinD1(#2922, 1:1000), rabbit anti-CyclinE(#20808, 1:1000). All-trans retinoic acid (ATRA) powder were purchased from Sigma, ATRA-releasing pellets were from Innovative Research of America. SPF BALB/c nude mice were raised in Laboratory Animal Center of Fujian Medical University. All of animal experiments were approved by Experimental Animal Ethics of Fujian Medical University.
Zhang Z.Z., Yu W.X., Zheng M., X.i.n.-.h.u.a.-.L.i.a.o., Wang J.C., Yang D.Y., Lu W.X., Wang L., Zhang S., Liu H.K., Zhou X.Z, & Lu K.P. (2019). Pin1 inhibition potently suppresses gastric cancer growth and blocks PI3K/AKT and Wnt/β-catenin Oncogenic pathways. Molecular carcinogenesis, 58(8), 1450-1464.
+ Open protocol
+ Expand
3

Western Blot Analysis of Metabolic Enzymes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse tissues were homogenized and lysed by using RIPA buffer (Santa Cruz Biotechnology). Protein contents were measured by using the Bradford assay (Pierce Biotechnology). Detailed steps for western blot analysis were as previously described59 (link). The primary antibodies included goat anti-KYNU, rabbit anti-KMO, mouse anti-KAT1, mouse anti-Cyclin B1, mouse anti-GAPDH (all above antibodies from Santa Cruz Biotechnology); mouse anti-YAP1, mouse anti-phospho-YAP (ser 127), rabbit anti-Erk1/2 (Thr202/Tyr204), rabbit anti-Erk1/2, rabbit anti-c-Myc, rabbit anti-phospho-Src (Tyr527) and rabbit anti-Src (all seven antibodies from Cell Signaling). The relative protein levels were calculated by normalizing to GAPDH protein as a loading reference by using ImageJ.
Zhang D., Ning J., Ramprasath T., Yu C., Zheng X., Song P., Xie Z, & Zou M.H. (2022). Kynurenine promotes neonatal heart regeneration by stimulating cardiomyocyte proliferation and cardiac angiogenesis. Nature Communications, 13, 6371.
+ Open protocol
+ Expand
4

Analyzing C-Myc and Cell Cycle Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
P493–6 cells were treated with DMSO, 0.1 μg ml−1 of Tet, 10 μM STR116 or 10 μM STR118 for 48 hours. Harvested cells were lysed in RIPA buffer, and protein concentration was determined using the BCA assay. Samples were loaded at equal protein concentration, separated by SDS-PAGE and transferred to nitrocellulose membranes (Amersham, 10600001). Membranes were incubated with rabbit anti-C-Myc (1:1,000, Cell Signaling Technology, 18583), mouse anti-CCNB1 (1:1,000, Cell Signaling Technology, 4135), rabbit anti-B-actin (1:4,000, Cell Signaling Technology, 4970) and rabbit anti-LDHA (1:4,000, Cell Signaling Technology, 3582). After washing, membranes were stained with IRDye-conjugated secondary antibodies (IRDye 680LT goat anti-mouse 1:10,000, LI-COR, 926–68020, and IRDye 800CW donkey anti-rabbit 1:10,000, LI-COR, 926–32213), and blots were visualized by LI-COR Odyssey imaging system.
Grobben G.J., Peters S.W., Wisselink H.W., Weusthuis R.A., Hoefnagel M.H., Hugenholtz J, & Eggink G. (2001). Spontaneous Formation of a Mannitol-Producing Variant of Leuconostoc pseudomesenteroides Grown in the Presence of Fructose. Applied and Environmental Microbiology, 67(6), 2867-2870.
+ Open protocol
+ Expand
5

Western Blot Characterization of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 50 μg of protein extracts, together with molecular weight markers, were subjected to 1D SDS-PAGE on 4%–12% gradient gels (Invitrogen). After electrophoresis per manufacturer’s manual (Invitrogen), proteins were transferred to PVDF membranes (Millipore) at 35 constant voltage for 1 hour using Invitrogen’s semidry blotting apparatus. Western analyses of PVDF membranes utilized established protocols and antibodies for DNMT1 (Abcam #Ab54759), mouse anti-flag (Sigma cat# F3165-.2MG), rabbit anti-PBRM1 (ABCAM cat# ab86156), rabbit anti c-MYC (Cell signaling cat# 5605), rabbit anti-p27/CDKN1B (Cell signaling Ab cat# 3833) and β-actin (Sigma, #a3854).
Gu X., Enane F., Tohme R., Schuerger C., Radivoyevitch T., Parker Y., Zuberi E., Przychodzen B., Jha B.K., Lindner D., Rini B, & Saunthararajah Y. (2021). PBRM1 loss in kidney cancer unbalances the proximal tubule master transcription factor hub to repress proximal tubule differentiation. Cell reports, 36(12), 109747.
+ Open protocol
+ Expand
6

Western Blot Analysis of Islet Cell Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For westerns, isolated islets or INS-1 cells grown in six well plates were homogenized in RIPA buffer and resolved on SDS-PAGE. Primary antibodies used were rabbit anti-c-Myc, 1:1000 (#5605, Cell Signaling, Boston, MA) and mouse anti-Gapdh, 1:5000 (#sc-32233, Santa Cruz). Secondary antibodies were anti-rabbit IRDye 800CW (#827-08365, Odyssey) and anti-mouse IRDye 680LT (#827-11080, Odyssey). Western analysis for cell cycle proteins on islets isolated from mice was carried out using the following antibodies—anti-Cdk1, 1:1000 (#9112, Cell Signaling Technologies), anti-Cdk2, 1:500 (#163, Santa Cruz Biotechnology), anti-Cdk4, 1:1000 (#260, Santa Cruz Biotechnology), anti-Cdk6, 1:500 (#3126, Abcam Inc.), anti-Cyclin A, 1:500 (#4710, Sigma), anti-Cyclin D3, 1:500 (#28283, Abcam Inc.), anti-Cyclin E, 1:500 (#481, Santa Cruz Biotechnology), anti-tubulin, 1:2000 (Calbiochem) and anti-actin, 1:2000 (Sigma).7 (link).
Puri S., Roy N., Russ H.A., Leonhardt L., French E.K., Roy R., Bengtsson H., Scott D.K., Stewart A.F, & Hebrok M. (2018). Replication confers β cell immaturity. Nature Communications, 9, 485.
+ Open protocol
+ Expand
7

Western Blot Characterization of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 50 μg of protein extracts, together with molecular weight markers, were subjected to 1D SDS-PAGE on 4%–12% gradient gels (Invitrogen). After electrophoresis per manufacturer’s manual (Invitrogen), proteins were transferred to PVDF membranes (Millipore) at 35 constant voltage for 1 hour using Invitrogen’s semidry blotting apparatus. Western analyses of PVDF membranes utilized established protocols and antibodies for DNMT1 (Abcam #Ab54759), mouse anti-flag (Sigma cat# F3165-.2MG), rabbit anti-PBRM1 (ABCAM cat# ab86156), rabbit anti c-MYC (Cell signaling cat# 5605), rabbit anti-p27/CDKN1B (Cell signaling Ab cat# 3833) and β-actin (Sigma, #a3854).
Gu X., Enane F., Tohme R., Schuerger C., Radivoyevitch T., Parker Y., Zuberi E., Przychodzen B., Jha B.K., Lindner D., Rini B, & Saunthararajah Y. (2021). PBRM1 loss in kidney cancer unbalances the proximal tubule master transcription factor hub to repress proximal tubule differentiation. Cell reports, 36(12), 109747.
+ Open protocol
+ Expand
8

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed for 20 min on ice with lysis buffer and centrifuged at 14,000× g for 10 min at 4 °C. Samples were resolved using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose membranes, blotted with appropriate primary antibodies at a dilution of 1:1000, and treated with peroxidase-conjugated secondary antibodies (Biosource International, Camarillo, CA, USA). Bound antibodies were visualized using chemiluminescent substrate (ECL; Amersham, Arlington Heights, IL, USA) and exposed to Kodak X-OMAT film (Kodak, New Haven, CT, USA). Rabbit anti-regenerating gene protein-3A (REG3A) was obtained from Abcam (ab95316). Primary antibodies including rabbit anti-phospho-p42/44 mitogen-activated protein kinase (MAPK), anti-phospho-Akt, rabbit anti-c-myc, rabbit anti-caspase 8, anti-caspase 9, and anti-caspase 7 (cleaved) were purchased from Cell Signaling Technology (Danvers, MA, USA). Goat anti-actin antibody was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Densitometric analyses were performed using Image J software (National Institutes of Health, Bethesda, MD, USA).
Cho Y., Park M.J., Kim K., Park J.Y., Kim J., Kim W, & Yoon J.H. (2020). Tumor-Stroma Crosstalk Enhances REG3A Expressions that Drive the Progression of Hepatocellular Carcinoma. International Journal of Molecular Sciences, 21(2), 472.
+ Open protocol
+ Expand
9

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were lysed for 20 min on ice with lysis buffer and centrifuged at 14000 g for 10 min at 4 °C. The samples were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes, blotted with the appropriate primary antibodies at a dilution of 1:1000, and treated with peroxidase-conjugated secondary antibodies (Biosource International, Camarillo, CA, United States). The bound antibodies were visualized using a chemiluminescent substrate (ECL; Amersham, Arlington Heights, IL, United States) and exposed to Kodak X-OMAT film (Kodak, New Haven, CT, United States). The primary antibodies, including rabbit anti-phospho-p42/44 MAPK, anti-phosphorylated-Akt, and rabbit anti-c-Myc, were purchased from Cell Signaling Technology (Danvers, MA, United States). The goat anti-β-actin antibody was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, United States). The densitometric analyses were performed with Image J software (National Institutes of Health, Bethesda, MD, United States).
Cho Y., Park M.J., Kim K., Kim S.W., Kim W., Oh S, & Lee J.H. (2020). Reactive oxygen species-induced activation of Yes-associated protein-1 through the c-Myc pathway is a therapeutic target in hepatocellular carcinoma. World Journal of Gastroenterology, 26(42), 6599-6613.
+ Open protocol
+ Expand
10

Immunoblot Analysis of Protein Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue or cell lysates were lysed with laemmli buffer containing 1% Triton, a protease inhibitor cocktail, and tyrosine and serine-threonine phosphorylation inhibitors. Lysates were subjected to immunoblot analysis using rabbit anti-V5 (ab15828; Abcam), rabbit anti-KLF15 (ABC471; Millipore), rabbit anti-GAPDH (MAB374; Millipore), rabbit anti-total Smad2/3 (3102S, Cell Signaling Technology), rabbit anti-phospho Smad2/3 (8828S, Cell Signaling Technology), mouse anti-α-SMA (A5228; Sigma), mouse anti-total β-catenin (BD610153), rabbit anti-phospho-β-catenin (ser552) (9566; Cell Signaling Technology), rabbit anti-c-Myc (5605s; Cell Signaling Technology), and rabbit anti-Wnt1 (365800; Invitrogen) as previously reported 36 (link).
Gu X., Mallipattu S.K., Guo Y., Revelo M.P., Pace J., Miller T., Gao X., Jain M.K., Bialkowska A.B., Yang V.W., He J.C, & Mei C. (2017). The loss of Krüppel-like factor 15 in Foxd1+ stromal cells exacerbates kidney fibrosis. Kidney international, 92(5), 1178-1193.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!