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Rat igg1 clone hrpn

Manufactured by BioXCell
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Rat IgG1 clone HRPN is a laboratory reagent used for the detection and quantification of rat IgG1 antibodies in various immunoassays. It functions as a detection antibody labeled with horseradish peroxidase (HRP), enabling colorimetric or chemiluminescent signal generation for the specific measurement of rat IgG1 levels.

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Lab products found in correlation

Rat igg2b clone ltf 2, by BioXCell (3 mentions) Anti il 6 clone mp5 20f3, by BioXCell (1 mentions) Anti csf1 clone 5a1, by BioXCell (1 mentions) L nil, by Enzo Life Sciences (1 mentions) Poly 1 c, by InvivoGen (1 mentions) Rat igg2a clone 2a3, by BioXCell (1 mentions) Ova323 339, by MBL Life Science (1 mentions) Anti ifnγ antibody clone xmg1, by BioXCell (1 mentions) Anti mouse ifn γ clone xmg1, by BioLegend (1 mentions) Rat igg2a, by BioXCell (1 mentions) Bleomycin, by Nippon Kayaku (1 mentions)

6 protocols using rat igg1 clone hrpn

1

Anti-CSF1 and Anti-IL-6 Modulation in Mice

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Mice were administered 200 µg of anti-CSF1 clone 5A1 (catalog no. BE0204; Bio X Cell) or Rat IgG1 clone HRPN (catalog no. BE0088; Bio X Cell) isotype control by the i.p. route in a 100 µl volume of PBS immediately post injury and then daily for 6 days. For combined M-CSF and IL-6 neutralization, mice were administered 400 µg of isotype, 200 µg of isotype and 200 µg of anti-CSF1, or 200 µg anti-CSF1 and 200 µg of anti-IL-6 clone MP5-20F3 (catalog no. BE0046; Bio X Cell) by the i.p. route in a 200 µl volume of PBS immediately post injury and then daily for 6 days.
Noel J.G., Ramser S.W., Pitstick L., Bonamer J.P., Mackenzie B., Seu K.G., Kalfa T.A., Cancelas J.A, & Gardner J.C. (2022). M-CSF supports medullary erythropoiesis and erythroid iron demand following burn injury through its activity on homeostatic iron recycling. Scientific Reports, 12, 1235.
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2

Modulation of CAR T Cell Therapy Efficacy

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Anakinra was administered intraperitoneally at 30mg/kg once per day for 5 days, starting 5 hours prior to CAR T cell transfer, or PBS (vehicle) for control mice. Anti murine IL-6 (clone MP5–20F3, BioXcell) or isotype (Rat IgG1, clone HRPN, BioXcell), anti murine IL-6R (clone 15A7, BioXcell) or isotype (Rat IgG2b, clone LTF-2, BioXcell) and anti murine IL-1b (clone B122, BioXcell) or isotype (Armenian Hamster IgG, clone Armenian Hamster IgG, BioXcell) were administered intraperitoneally once per day at 25mg/kg for the first dose and 12.5mg/kg for subsequent doses for 5 days starting 5 hours prior to CAR T cell transfer. L-NIL (Enzo Life Sciences) or 1400W (Cayman Chemical) were administered intraperitoneally at 5mg/kg once per day for 5 days starting 5 hours prior to CAR T cell transfer, or PBS (vehicle) for control mice.
Giavridis T., van der Stegen S.J., Eyquem J., Hamieh M., Piersigilli A, & Sadelain M. (2018). CAR T cell-induced cytokine release syndrome is mediated by macrophages and abated by IL-1 blockade. Nature medicine, 24(6), 731-738.
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3

Immunotherapy Reagents and Protocols

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Peptides OVA257-264 and OVA323-339 were purchased from MBL International and GenScript. Poly (I:C) and CpG were obtained from Invivogen and reconstituted according to manufacturer's protocol. Anti-mouse GITR antibody (aGITR, clone DTA-1), anti-mouse CD279 antibody (aPD-1, clone RMP1-14), anti-mouse CD4 (aCD4, clone GK1.5), anti-mouse CD8 (aCD8, clone 53-6.72), anti-mouse CD25 antibody (aCD25, clone PC-61.5.3), anti-mouse NK1.1 (aNK1.1, clone PK136), anti-mouse/human killer cell lectin-like receptor subfamily G, member 1 (aKLRG1, clone 2F1; hamster antibody) and control antibodies (rat IgG2A, Clone 2A3; rat IgG2b, Clone LTF-2; rat IgG1, clone HRPN) and hamster IgG (BE0087) were purchased from BioXcell (West Lebanon, NH).
Villarreal D.O., Chin D., Smith M.A., Luistro L.L, & Snyder L.A. (2017). Combination GITR targeting/PD-1 blockade with vaccination drives robust antigen-specific antitumor immunity. Oncotarget, 8(24), 39117-39130.
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4

Treatment of Lung Tumors with LNT and Anti-IFNγ

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When LAP0297 lung tumors reached 4–5 mm in diameter, mice bearing tumors were randomly divided into four groups, which were treated with an isotype-matched control rat IgG1 (clone HRPN, Bio-X Cell), 1 mg/kg LNT, 250 μg anti-IFNγ antibody (clone XMG1.2, Bio-X Cell), or LNT plus an anti-IFNγ antibody. LNT was administered into mice via i.p. injection every 3 days, while rat IgG1 or anti-IFNγ antibody were given every 3 days. The treatment duration was 10 days.
Deng S., Zhang G., Kuai J., Fan P., Wang X., Zhou P., Yang D., Zheng X., Liu X., Wu Q, & Huang Y. (2018). Lentinan inhibits tumor angiogenesis via interferon γ and in a T cell independent manner. Journal of Experimental & Clinical Cancer Research : CR, 37, 260.
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5

Depletion of IL-2 and IFN-γ in mice

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IL-2 was depleted by i.p. administration of 100 µg anti-mouse IL-2 (clones S4B6-1 and JES6-5H4; BioXCell) on days −1, +2, and +5 p.i. Control animals were dosed to the same schedule with 100 µg rat IgG2a (clones LTF-2 and 2A3; BioXCell). IFN-γ was depleted by i.p. administration of 100 µg anti-mouse IFN-γ (clone XMG1.2; BioLegend) on days −1, +2, and +5 p.i., while control animals received 100 µg rat IgG1 (clone HRPN; BioXCell).
Pyle C.J., Labeur-Iurman L., Groves H.T., Puttur F., Lloyd C.M., Tregoning J.S, & Harker J.A. (2021). Enhanced IL-2 in early life limits the development of TFH and protective antiviral immunity. The Journal of Experimental Medicine, 218(12), e20201555.
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6

Anti-mCCL6 and Anti-mMARCO Antibody Treatments

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The anti-mCCL6 mAb (Clone 262,016, R&D, Abingdon, UK) or anti-mMARCO mAb (Clone ED31, GeneTex, Alton Pkwy Irvine, CA, USA) was injected i.p. into the aged mice (100 μg/mouse in 100 μL of PBS) 7 days before bleomycin (Nippon Kayaku Co., Ltd, Takasaki-shi, Japan) challenge, and additional injections were performed every 7 days. Control mice were administrated equal amounts of control antibody Rat IgG2b (clone LTF-2; BioXcell, West Lebanon, NH, USA) or Rat IgG1 (clone HRPN, BioXcell, West Lebanon, NH, USA) respectively.
Chen Y., Hao X., Li M., Tian Z, & Cheng M. (2023). UGRP1-modulated MARCO+ alveolar macrophages contribute to age-related lung fibrosis. Immunity & Ageing : I & A, 20, 14.
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