3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium mtt

All chemicals (reagent grade) used were purchased from Nanjing Chemical Reagent Co. Ltd. (Nanjing, China). All the ¹H NMR spectra were recorded on a Bruker DPX 300 model Spectrometer in CDCl₃ and chemical shifts (Δ) were reported as parts per million (ppm). ESI-MS spectra were recorded a Mariner System 5304 Mass spectrometer. Elemental analyses were performed on a CHNO-Rapid instrument and were within 0.4% of the theoretical values. Thin layer chromatography (TLC) was performed on silica gel plates (Silica Gel 60 GF254) and visualized in UV light (254 nm). Column chromatography was performed using silica gel (200–300 mesh) eluting with ethyl acetate and petroleum ether (bp. 30–60 °C).
STAT3 Antibody (#12640), phospho-STAT3 (Tyr705) Antibody (#9131) and GAPDH Antibody (#2118) were purchased from Cell Signaling Technology (Beverly, MA). BCL-XL Antibody (WL01558), Bcl-2 Antibody (WL01556), Survivin Antibody (WL01684), Cyclin D1 Antibody (WL01435a), β-actin Antibody (WL01774), Lamin B Antibody (WL01775), and Goat anti-rabbit IgG (H + L) (WLA023a) were purchased from Wanleibio Co., Ltd. (Shenyang, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) were purchased from Sigma-Aldrich (St. Louis, USA). Annexin V-FITC Apoptosis Detection Kit (A211-01/02) was purchased from Vazyme Biotech Co.,Ltd (Nanjing, China).

MCF-7 Cell Line, noninvasive estrogen receptor (ER) positive was prepared from the Pasteur Institute (Tehran, IRAN). Trypsin, EGCG, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium (MTT) were purchased from Sigma (Germany). EGCG dissolved in dimethyl sulfoxide (DMSO, Solon, OH, USA). Arsenic Trioxide (ATO) was purchased from Merck (Germany) and dissolved in Phosphate buffered saline (PBS) (Gibco, UK), at a concentration of 1mM at a stock solution and diluted before use.

Both Iscove’s Modified Dulbecco’s Medium (IMDM) and trypsin (0.25%) were purchased from Thermo Fisher Scientific (MA, United States). Fetal bovine serum (FBS) was purchased from CellMax (Beijing, China). Phosphate-buffered saline (PBS) was purchased from BasalMedia (Shanghai, China). MIA, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (Taufkirchen, Germany). Cell culture plates were purchased from Corning (NY, United States), and transwell chambers from Eppendorf (Hamburg, Germany). DNase I and TRIzol reagent were purchased from TaKaRa Biotechnology Co., Ltd. (Dalian, China). The cDNA Synthesis SuperMix kit was purchased from Biotool (TX, United States). The SYBR Green (2×) qPCR Master Mix (low ROX) kit was purchased from Bimake (TX, United States). Collagenase type I (0.2%) and Collagenase type II (0.2%) were purchased from Worthington Biochemical Corp. (NJ, United States). All primary and secondary antibodies were obtained from Cell Signaling Technology Inc. (MA, United States).

HeLa cells (Human Cervical cancer) and AC-16 (Human cardiomyocyte), were gifted from Dr Mawieh, Sharjah university, Sharjah. HeLa and AC-16 cells were maintained in DMEM with 10% FBS (Sigma; St. Louis, MO, USA) with 1% penicillin and streptomycin procured from Sigma (Sigma; St. Louis, MO, USA) in a humidified environment with 5% CO₂ at a constant temperature of 37 °C. 4′,6-diamidino-2-phenylindole (DAPI), dimethyl sulfoxide (DMSO), propidium iodide (PI), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium (MTT) and trypsin-EDTA were obtained from Sigma Aldrich (Merck KGaA).