Milli q gradient a10 system

Manufactured by Merck Group

Sourced in United States, France, Germany, United Kingdom

The Milli-Q Gradient A10 system is a water purification system designed to produce ultrapure water for critical laboratory applications. The system utilizes a multi-stage purification process to remove impurities and contaminants, producing water that meets the highest quality standards. The core function of the Milli-Q Gradient A10 is to provide a consistent and reliable supply of ultrapure water for various laboratory procedures and experiments.

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Lab products found in correlation

Formic acid, by Merck Group (11 mentions) Acetonitrile, by Merck Group (6 mentions) Acetic acid, by Merck Group (5 mentions) Methanol, by Merck Group (5 mentions) Chloroform, by Merck Group (5 mentions) Fecl3 6h2o, by Merck Group (4 mentions) Oleic acid, by Merck Group (4 mentions) Phosphate buffered saline (pbs), by Merck Group (4 mentions) Hydrochloric acid (hcl), by Merck Group (4 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Dichloromethane, by Tianjin Damao (3 mentions) Ammonium acetate nh 4 ch 3 co 2, by Thermo Fisher Scientific (2 mentions) Ammonium bicarbonate, by Merck Group (2 mentions) Leucine enkephalin, by Merck Group (2 mentions) Fecl2 4h2o, by Merck Group (2 mentions) Ethanol, by Merck Group (2 mentions) Ferric chloride hexahydrate, by Merck Group (2 mentions) Citric acid, by Merck Group (2 mentions) Perchloric acid, by Avantor (2 mentions) Ethanol, by Lach-Ner (2 mentions)

89 protocols using milli q gradient a10 system

Pesticide Analysis in Agricultural Commodities

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Standard stock solutions of the pesticides subjected to official control in agricultural commodities (EC, 2019a) were purchased from CPA Chem (Stara Zagora, Bulgaria) in 10 compatible mixes (10 μg mL -1 each). The rest of the pesticides and the isotopically labeled Procedural Internal Standards (P-IS) were purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany) and Sigma-Aldrich (Büchs, Switzerland), all with a purity superior to 97.1%.
LC-MS grade formic acid (FA, HCOOH), acetic acid (AA, CH 3 COOH), acetonitrile (ACN) and methanol (MeOH) were purchased from Honeywell (Morristown, NJ). Ultrapure water was produced in the laboratory using a Gradient A10 Milli-Q System (Millipore, Bedfore, MA, USA). AOAC and EN method QuEChERS salts, and Agilent d-SPE Enhanced Matrix Removal-Lipid (EMR-lipid) were acquired from Agilent Technologies (Palo Alto, USA). Ammonium acetate (NH 4 CH 3 CO 2 ) and ammonium formate (NH 4 CO 2 ) were obtained from Fisher Scientific (Loughborough, UK).

Acosta-Dacal A., Rial-Berriel C., Díaz-Díaz R., Bernal-Suárez M.D.M, & Luzardo O.P. (2021). Optimization and validation of a QuEChERS-based method for the simultaneous environmental monitoring of 218 pesticide residues in clay loam soil. The Science of the total environment, 753.

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Quantitative Analysis of Agrochemicals

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Individual certified standards of ARs and PhACs (purity 95.19% to 99.9%) were acquired from Dr. Ehrenstorfer (Augsburg, Germany), Sigma-Aldrich (Augsburg, Germany) and European Pharmacopoeia Reference Standards (Strasbourg, France). Atrazine-d5, Carbendazim-d3, Coumachlor, Cyromazine-d4, Linuron-d3 and Pirimicarb-d6 (Dr. Ehrenstorfer and Sigma-Aldrich, 99.3–99.9% purity) were used as procedural internal standards (P-IS) and were maintained as described in the reference method [41 (link)]. These internal standards were added to the samples at the beginning of the procedure to account for various sources of errors throughout all stages in the method [58 ]. The list of the selected analytes, their classification, and their legal status are in Table 1.
LC-MS grade methanol (MeOH), acetonitrile (ACN) and formic acid (FA, HCOOH) were obtained from Honeywell (Morristown, NJ, USA). Ammonium acetate (NH₄CH₃CO₂) was purchased from Fisher Scientific (Loughborough, UK). AOAC method QuEChERS salts [59 (link)] (6 g of MgSO₄ and 1.5 g of CH₃COONa) were acquired in commercial premixes from Agilent Technologies (Palo Alto, CA, USA). The ultrapure water was produced in the laboratory using a Gradient A10 Milli-Q System (Millipore, Bedfore, MA, USA).

Acosta-Dacal A., Rial-Berriel C., Díaz-Díaz R., Bernal-Suárez M.D., Zumbado M., Henríquez-Hernández L.A, & Luzardo O.P. (2021). An Easy Procedure to Quantify Anticoagulant Rodenticides and Pharmaceutical Active Compounds in Soils. Toxics, 9(4), 83.

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Thiol-Activated Fluorescent Labeling

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3-Mercaptopropionic acid (≥99%), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM·HCl) (96%) and rhodamine B (97%) were purchased from Aldrich. Phosphate-buffered saline (PBS) was purchased from Scharlau. 4-(Dimethylamino)pyridine (DMAP) and tobramycin (97%) were purchased from Acros-Organics. DNase I was purchased from Panreac, Applichem. The water (H₂O) used in the syntheses was deionized water from a Milli-Q A10 Gradient system (Millipore).

Blanco-Cabra N., Movellan J., Marradi M., Gracia R., Salvador C., Dupin D., Loinaz I, & Torrents E. (2022). Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm. NPJ Biofilms and Microbiomes, 8, 52.

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Deer Antler Peptide Biomarker Discovery

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Trypsin (proteomics grade), guanidine hydrochloride, hydroxymethyl aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), ammonium bicarbonate, and acetic acid (biotech grade) were purchased from Sigma-Aldrich (St. Louis, MO, USA), formic acid (optima LCMS) was obtained from Thermo Fisher Scientific (Waltham, MA, USA), acetonitrile (gradient grade) and methanol (gradient grade) was purchased from Merck KGaA (Darmstadt, Germany). Ultrafiltration centrifuge tubes (3 kDa, 0.5 mL) were purchased from Merck Millipore (Burlington, MA, USA). Water was prepared on a Milli-Q A 10 Gradient system by Millipore (Schwalbach, Germany).
Twenty-three batches of deer antlers were collected from markets in different regions of China. The species of deer antlers were identified by the Shandong Academy of Agricultural Sciences using the DNA barcode method; 3 batches of reindeer antlers were identified by the method provided by literature [3 (link)], and the sample information is shown in Table S1. Seven species of deer antlers were used to discover peptide biomarkers, and all samples were used for validation. The peptide biomarkers shown in Table 1 were chemically synthesized by GL Biochem (Shanghai, China), Yuan Peptide (Nanjing, China) and China peptides (Suzhou, China).

Xue F., Wang B., Guo D.X., Jiao Y., Yin X., Cui W.L., Zhou Q.Q., Yu F.R, & Lin Y.Q. (2022). Peptide Biomarkers Discovery for Seven Species of Deer Antler Using LC-MS/MS and Label-Free Approach. Molecules, 27(15), 4756.

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Electrochemical Detection of ABA

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Standards, such as carbofuran (98.5%), isoprocarb (99.9%), methomyl (98.3%), pirimicarb (99.0%), aldicard (98.5%) and metholcarb (1000 mg L⁻¹ in acetone) were purchased from the Shanghai Pesticide Research Institute (Shanghai, China). p-Aminobenzoic acid (99.0%) (ABA) was purchased from J&K Scientific Ltd. (Shanghai, China). HPLC-grade methanol was purchased from Merck (Darmstadt, Germany). Acetic acid, H₂SO₄, Na₂HPO₄, NaH₂PO₄, K₃Fe(CN)₆, K₄Fe(CN)₆ and KCl were of analytical purity. Water used was purified using a Milli-Q gradient A10 system (Millipore Corp., Billerica, MA, USA). A stock solution of ABA (100 mmol L⁻¹) was prepared in methanol. A 0.05 mol L⁻¹ phosphate buffered solution (PBS, pH = 7.0) was prepared with Na₂HPO₄ and NaH₂PO₄ solutions. The supporting electrolytewas1 mmol L⁻¹ K₃Fe (CN)₆/K₄Fe(CN)₆ (1 : 1) containing 0.1 mol L⁻¹ KCl solution for the detection of the analyte.

Qi P., Wang J., Wang X., Wang Z., Xu H., Di S., Wang Q, & Wang X. (2018). Sensitive and selective detection of the highly toxic pesticide carbofuran in vegetable samples by a molecularly imprinted electrochemical sensor with signal enhancement by AuNPs. RSC Advances, 8(45), 25334-25341.

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Analytical Standards for Ciguatoxin Detection

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C-CTX1 pure standard solution (5 ng·mL⁻¹) was kindly provided by Dr. Robert Dickey (previously, U.S. Food and Drug Administration) via Dr. Ronald Manger (Fred Hutchinson Cancer Research Center, Seattle, USA). A qualitative mixture of P-CTXs standard solution containing: CTX1B, 2,3-dihydroxyCTX3C, 51-hydroxyCTX3C, 52-epi-54-deoxyCTX1B/54-deoxyCTX1B, 2-hydroxyCTX3C, 49-epiCTX3C, CTX3C, CTX4A and CTX4B, was kindly provided by Prof. Takeshi Yasumoto (Japan Food Research Laboratories, Japan)
Acetone, diethyl ether, methanol, water, hexane and ethyl acetate used for extraction and purification were of HPLC grade (Merck KGaA, Darmstadt, Germany). Methanol, Acetonitrile, formic acid, ammonium formiate (Merck KGaA, Darmstadt, Germany) and water (J. T. Baker, Center Valley, PA, USA) for LC-MS analysis were of LC-MS grade. Methanol, formic acid, ammonium formiate (Merck KGaA, Darmstadt, Germany) for HPLC fractionation were of HPLC grade. Water for fractionation was deionized and purified at 15 MΩ·cm⁻¹ through a Milli-Q Gradient A10 system (Millipore, Molsheim, France)

Estevez P., Castro D., Pequeño-Valtierra A., Leao J.M., Vilariño O., Diogène J, & Gago-Martínez A. (2019). An Attempt to Characterize the Ciguatoxin Profile in Seriola fasciata Causing Ciguatera Fish Poisoning in Macaronesia. Toxins, 11(4), 221.

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Lipid Monolayer Film Formation

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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC, R-enantiomer), palmitic acid (PA) and dihydrocholesterol (Chol) (Avanti, Alabaster, AL) with 0.1 wt% Texas-Red DHPE (N- (Texas Red sulfonyl) -1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, Invitrogen, Grand Island, NY) were mixed in the appropriate ratios and diluted to ∼0.2 mg/ml in HPLC-grade chloroform (Fisher Scientific, St. Louis, MO) to form a spreading solution. Dihydrocholesterol was used instead of cholesterol to minimize oxidation, but the phase behaviorand domain formationis similar to cholesterol (51 ). The spreading solution was deposited dropwise onto the clean air-water interface from a Hamilton syringe (Reno, Nevada). 20 minutes were allowed for solvent evaporation prior to film compression. A Milli-Q Gradient A10 system (Millipore, Billerica, MA) provided ultrapure water with a resistivity of at least 18.0 MΩ·cm.

Sachan A.K., Choi S.Q., Kim K.H., Tang Q., Hwang L., Lee K.Y., Squires T.M, & Zasadzinski J.A. (2017). Interfacial Rheology of Coexisting Solid and Fluid Monolayers. Soft matter, 13(7), 1481-1492.

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Analytical Protocol for Thiamethoxam Analysis

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During this study, ultrapure water with a resistivity of 18.2 M Ω cm was produced by a Milli-Q Gradient A-10 system (Millipore). The commercial TMX formulation (25% w/w) was provided by Syngenta, while the active principle TMX (≥98%) was purchased from Sigma-Aldrich (Steinheim, Germany). The chemical ingredients used to prepare the minimal salt medium MSM1 [66 (link)], MSM2 [67 (link)], and MSM3 [68 (link)] were of analytical grade (Sigma-Aldrich Chemie, Steinheim, Germany; Merck, Darmstadt, Germany). Sodium acetate and acetonitrile HPLC grade were provided by Merck (Darmstadt, Germany). Trifluoroacetic acid 99% was purchased from Sigma-Aldrich (Steinheim, Germany). Microfiber glass filters of 0.45 µm (Whatman™, Maidstone, UK) were used to filter HPLC grade solvents. All other chemicals and solvents used were of the highest purity and of analytical grade.

Boufercha O., Monforte A.R., Boudemagh A., Ferreira A.C., Castro P.M, & Moreira I.S. (2022). Biodegradation and Metabolic Pathway of the Neonicotinoid Insecticide Thiamethoxam by Labrys portucalensis F11. International Journal of Molecular Sciences, 23(22), 14326.

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Enantiopure Serine Analysis by 2D-HPLC

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The enantiomer of serine and HPLC-grade acetonitrile were obtained from Nacalai Tesque (Kyoto, Japan). Methanol of HPLC grade, trifluoroacetic acid (TFA), citric acid monohydrate, and boric acid were purchased from Wako (Osaka, Japan). Water was purified using a Milli-Q gradient A 10 system (Millipore, Bedford, MA, USA). All other reagents for 2D-HPLC were of the highest reagent grade and were used without further purification.

Sasabe J., Suzuki M., Miyoshi Y., Tojo Y., Okamura C., Ito S., Konno R., Mita M., Hamase K, & Aiso S. (2014). Ischemic Acute Kidney Injury Perturbs Homeostasis of Serine Enantiomers in the Body Fluid in Mice: Early Detection of Renal Dysfunction Using the Ratio of Serine Enantiomers. PLoS ONE, 9(1), e86504.

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Analytical Procedures for Sample Analysis

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All chemicals for sample analyses (from the pre-analytical step to the data acquisition stage) were of analytical or pure grade quality and obtained from various international suppliers. Briefly, the organic solvents used, methanol and acetonitrile, were LCMS grade quality (Romil, MicroSep, South Africa). Water was purified by a Milli-Q Gradient A10 system (Millipore, Billerica, MA, USA). Leucine enkephalin and formic acid were purchased from Sigma Aldrich, Steinheim am Albuch, Germany. The study design and plants’ cultivation are detailed in the following section.

Tinte M.M., Masike K., Steenkamp P.A., Huyser J., van der Hooft J.J, & Tugizimana F. (2022). Computational Metabolomics Tools Reveal Metabolic Reconfigurations Underlying the Effects of Biostimulant Seaweed Extracts on Maize Plants under Drought Stress Conditions. Metabolites, 12(6), 487.

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