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Parp2 chemiluminescent assay kit

Manufactured by BPS Biosciences
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Sourced in United States

The PARP2 Chemiluminescent Assay Kit is a laboratory equipment product designed to measure the activity of the PARP2 enzyme. The kit utilizes a chemiluminescent detection method to quantify PARP2 activity in a simple and efficient manner.

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Lab products found in correlation

Universal chemiluminescent parp assay kit, by Bio-Techne (2 mentions) Synergy microplate reader, by Agilent Technologies (2 mentions) Envision 2104 multilabel microplate reader, by PerkinElmer (1 mentions)

3 protocols using parp2 chemiluminescent assay kit

1

PARP Enzyme Inhibition Assay

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PARP1 and PARP2 IC50 for Rucaparib, Veliparib and the various CRBN-based PARP degraders were measured by using a PARP universal chemiluminescent assay kit (Trevigen, #4676–096-K) and PARP2 Chemiluminescent Assay Kit (BPS Bioscience, #80552), respectively. Briefly, the histone-coated strip wells (PARP1) were hydrated with the assay buffer for 30 min. The blank strip wells (PARP2) were coated with histone proteins overnight at 4℃ and blocked with blocking buffer for 90 min. The various compounds were added and were incubated with the PARP enzyme for 10 min. The reaction cocktail containing biotinylated NAD+ was then added into the system, which was incubated for 1 hour to activate PARP enzyme. After the reaction, the wells were washed twice with 0.1% Triton X-100/PBS and twice with PBS (PARP1) or were washed three times with 0.05% Tween-20/PBS (PARP2). The wells were incubated with Strep-HRP for another hour, and were washed again as described above. Peroxy-Glow reagents were mixed and added into the strip wells, and the biotin signal on coated histone was immediately measured with chemiluminescence on a Synergy microplate reader (Bio-Tek). The well without the PARP enzyme, and the well with the PARP enzyme only was used as the negative (0%) and positive (100%) control, respectively.
Wang S., Han L., Han J., Li P., Ding Q., Zhang Q.J., Liu Z.P., Chen C, & Yu Y. (2019). Uncoupling of PARP1 Trapping and Inhibition Using Selective PARP1 Degradation. Nature chemical biology, 15(12), 1223-1231.
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2

PARP Enzyme Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
PARP1 and PARP2 IC50 for Rucaparib, Veliparib and the various CRBN-based PARP degraders were measured by using a PARP universal chemiluminescent assay kit (Trevigen, #4676–096-K) and PARP2 Chemiluminescent Assay Kit (BPS Bioscience, #80552), respectively. Briefly, the histone-coated strip wells (PARP1) were hydrated with the assay buffer for 30 min. The blank strip wells (PARP2) were coated with histone proteins overnight at 4℃ and blocked with blocking buffer for 90 min. The various compounds were added and were incubated with the PARP enzyme for 10 min. The reaction cocktail containing biotinylated NAD+ was then added into the system, which was incubated for 1 hour to activate PARP enzyme. After the reaction, the wells were washed twice with 0.1% Triton X-100/PBS and twice with PBS (PARP1) or were washed three times with 0.05% Tween-20/PBS (PARP2). The wells were incubated with Strep-HRP for another hour, and were washed again as described above. Peroxy-Glow reagents were mixed and added into the strip wells, and the biotin signal on coated histone was immediately measured with chemiluminescence on a Synergy microplate reader (Bio-Tek). The well without the PARP enzyme, and the well with the PARP enzyme only was used as the negative (0%) and positive (100%) control, respectively.
Wang S., Han L., Han J., Li P., Ding Q., Zhang Q.J., Liu Z.P., Chen C, & Yu Y. (2019). Uncoupling of PARP1 Trapping and Inhibition Using Selective PARP1 Degradation. Nature chemical biology, 15(12), 1223-1231.
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3

PARP-1 and PARP-2 Inhibition Assays

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The PARP-1 and PARP-2 inhibition assays were performed in outsourcing, by a CRO company, Shanghai Medicilon Inc. (Shanghai, China). The PARP-1 and PARP-2 inhibitory activities of the compounds were measured using PARP-1 Chemiluminescent Assay Kit (BPS Bioscience, catalog 80569, San Diego, CA, USA) and PARP-2 Chemiluminescent Assay Kit (BPS Bioscience, catalog 80552), respectively, according to the manufacturer’s instructions. Briefly, PARP-1 or PARP-2 biotinylated substrate was incubated with compounds or solvent control at varying concentrations and an assay buffer containing the PARP-1 or PARP-2 enzyme. After then, the plate was treated with streptavidin-HRP followed by addition of the HRP substrate and the luminescent signal was measured using a chemiluminescence reader (Perkin Elmer Envision 2104 Multi Label Microplate Reader, Waltham, MA, USA).
Min R., Wu W., Wang M., Tang L., Chen D., Zhao H., Zhang C, & Jiang Y. (2019). Discovery of 2-(1-(3-(4-Chloroxyphenyl)-3-oxo- propyl)pyrrolidine-3-yl)-1H-benzo[d]imidazole-4-carboxamide: A Potent Poly(ADP-ribose) Polymerase (PARP) Inhibitor for Treatment of Cancer. Molecules, 24(10), 1901.
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