Anti flotillin 1 antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The anti flotillin-1 antibody is a research tool used to detect and study the flotillin-1 protein. Flotillin-1 is a membrane-associated protein involved in various cellular processes. This antibody can be used to identify and localize flotillin-1 in biological samples through techniques like Western blotting and immunohistochemistry.

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Lab products found in correlation

Anti β actin antibody, by Santa Cruz Biotechnology (1 mentions) Anti cd81 antibody, by Santa Cruz Biotechnology (1 mentions) Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg h l, by Abcam (1 mentions) Anti cd9 antibody, by Santa Cruz Biotechnology (1 mentions) Anti tsg101 antibody, by Santa Cruz Biotechnology (1 mentions) Anti cd63 antibody, by Santa Cruz Biotechnology (1 mentions) Anti calnexin antibody, by Santa Cruz Biotechnology (1 mentions) Anti ctsk antibody, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-Flotillin-1 (14 citations) Flotillin-1 (13 citations)

3 protocols using anti flotillin 1 antibody

Flotillin-1 Antibody Labeling of Membranes

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Purified membrane samples were incubated with anti flotillin-1 antibody (Santa Cruz Biotechnology, USA). The antibody was diluted 1:4 in recording buffer (to 50 μg/ml), and the sample was imaged after 60min of the antibody incubation. The difference of the surface area of the membrane samples visualized in AFM images, before and after the antibody incubation, was determined following the procedures reported elsewhere [31 (link)].

Cremona A., Orsini F., Corsetto P.A., Hoogenboom B.W, & Rizzo A.M. (2015). Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature. PLoS ONE, 10(7), e0132696.

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Exosome Protein Analysis by Western Blot

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EV or NV (10 µg) was separated by 10% SDS-PAGE and shifted to a polyvinylidene difluoride membrane. The membrane blocked in nonfat milk was incubated with anti-flotillin-1 antibody (Santa Cruz Biotechnology), anti-CD81 antibody (Santa Cruz Biotechnology), or anti-beta-actin antibody (Santa Cruz Biotechnology). The specific bands were detected with chemiluminescent substrates following treatment with secondary antibody conjugated with horseradish peroxidase.

Bandeira E., Jang S.C., Lässer C., Johansson K., Rådinger M, & Park K.S. (2023). Effects of mesenchymal stem cell-derived nanovesicles in experimental allergic airway inflammation. Respiratory Research, 24, 3.

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Protein Extraction and Western Blot Analysis

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Proteins were extracted from the ultracentrifugation pellets and separated on a denatured SDS–polyacrylamide gel before transfer to a polyvinylidene difluoride membrane. The blotting membrane was blocked with bovine serum albumin and incubated with goat polyclonal anti-Sema4D antibody (1:100; Santa Cruz, sc-16632), rabbit polyclonal anti-CTSK antibody (1:100; Santa Cruz, sc-30056), goat polyclonal anti-tartrate-resistant acid phosphatase antibody (1:100; Santa Cruz, sc-30833), rabbit polyclonal anti-CD9 antibody (1:100; Santa Cruz, sc-9148), rabbit polyclonal anti-CD63 antibody (1:100; Santa Cruz, sc-15363), rabbit polyclonal anti-Flotillin-1 antibody (1:100; Santa Cruz, sc-25506), goat polyclonal anti-TSG101 antibody (1:100; Santa Cruz, sc-6037) and rabbit polyclonal anti-calnexin antibody (1:100; Santa Cruz, sc-11397) followed by incubation with horseradish peroxidase (HRP)-coupled goat anti-rabbit IgG H&L (1:5,000, Abcam, ab6721) or HRP-coupled rabbit anti-goat IgG H&L (1:5,000, Abcam, ab6741), respectively. The proteins were detected using SuperSignal West Dura Extended Duration Substrate (Thermo Scientific, Prod # 34075). Supplementary Fig. 17 depicts uncropped western blots.

Li D., Liu J., Guo B., Liang C., Dang L., Lu C., He X., Cheung H.Y., Xu L., Lu C., He B., Liu B., Shaikh A.B., Li F., Wang L., Yang Z., Au D.W., Peng S., Zhang Z., Zhang B.T., Pan X., Qian A., Shang P., Xiao L., Jiang B., Wong C.K., Xu J., Bian Z., Liang Z., Guo D.A., Zhu H., Tan W., Lu A, & Zhang G. (2016). Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation. Nature Communications, 7, 10872.

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