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Everolimus

Manufactured by Apexbio
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Everolimus is a laboratory product used for research purposes. It is a macrolide compound that functions as a mTOR inhibitor.

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Lab products found in correlation

Cck 8 kit, by BestBio (1 mentions) A1010, by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Gapdh, by Proteintech (1 mentions) Carbenoxolone, by Merck Group (1 mentions) Mefloquine, by Merck Group (1 mentions) Arsenic trioxide, by Merck Group (1 mentions) Danusertib, by Apexbio (1 mentions)

4 protocols using everolimus

1

Evaluating Everolimus Efficacy in METTL3 Models

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For the in vitro study, everolimus (APExBIO, Houston, TX, USA) was added to cells with final concentrations of 5 or 50 µg/mL. Cell viability was measured using a CCK-8 kit (BestBio, Shanghai, China). For the in vivo study, subcutaneous xenograft models with METTL3-high (n = 3) and control cells (n = 3) were established as above. Control cells were inoculated two days before METTL3-high cells. When the tumor sizes were similar, volume-matched mice received everolimus (50 µg/day intragastrically) or solvent for 17 days and were sacrificed at day 18. Mice were randomly allocated to each group with no blinding.
Sun Y., Li S., Yu W., Zhao Z., Gao J., Chen C., Wei M., Liu T., Li L, & Liu L. (2020). N6-methyladenosine-dependent pri-miR-17-92 maturation suppresses PTEN/TMEM127 and promotes sensitivity to everolimus in gastric cancer. Cell Death & Disease, 11(10), 836.
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2

Everolimus and Arsenic Trioxide Hormone Deprivation

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Everolimus (A8169, ApexBio), and arsenic trioxide (A1010, Sigma) were administrated at the concentration of 1nM and 100 nM, respectively. For hormone deprivation, cell or BICA cultures were cultured with 2–5% charcoal-striped FBS and phenol-red free DMEM/F12 medium. When androgen supply was needed, testosterone (given by Dr. David Rowley’s lab at Baylor College of Medicine) was added at the working concentration of 10nM.
Wu Y.H., Gugala Z., Barry M.M., Shen Y., Dasgupta S, & Wang H. (2022). Optimization and characterization of a bone culture model to study prostate cancer bone metastasis. Molecular cancer therapeutics, 21(8), 1360-1368.
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3

Immunoblotting Reagent Preparation and Use

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Antibodies were used in the following dilutions: SLC3A2 (1:1000, Affinity, #DF7468), FKBP1A (1:1000, Proteintech, #10273-1-AP), SLC7A11 (Abcam, ab37185), GPX4 (Abcam, ab125066), IL-9R (Santa Cruz, sc-515622), GAPDH (1:1000, Proteintech, #60004-1-Ig), HA (1:1000, Proteintech, #51064-2-AP), Flag (1:1000, Sigma, #F3165). Secondary antibody goat anti-mouse (1:2500, HuaBio, #HA1006), secondary antibody goat anti-rabbit (1:2500, HuaBio, #HA1001). Everolimus was purchased from APExBIO (#A8169), and resolved in DMSO at 10mM. Erastin was purchased from meilunbio (#MB4114), and resolved in DMSO at 10mM. IL-9 was purchased from MCE (#HY-P7046), and resolved in PBS at 100μg/mL.
Chen Z., Li R., Fang M., Wang Y., Bi A., Yang L., Song T., Li Y., Li Q., Lin B., Jia Y., Fu S., Fu S, & Xiong H. (2023). Integrated analysis of FKBP1A/SLC3A2 axis in everolimus inducing ferroptosis of breast cancer and anti-proliferation of T lymphocyte. International Journal of Medical Sciences, 20(8), 1060-1078.
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4

Pharmacological Interventions in Cancer Models

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The administration of Carbenoxolone, Mefloquine and Danusertib
followed previous reports (Fraedrich et al.,
2012; Keiser et al., 2011 (link);
Morecki et al., 1997 (link)) whereas the
dose of the two FDA-approved drugs, Everolimus and Arsenic trioxide were
adjusted from clinical human dose by FDA guidance: mouse dose(mg/kg)=human
dose X mouse Km (~37) / mouse Km (~3). Carbenoxolone (CBX,
C4790 Sigma) was injected i.p. daily at the dosage of 30 mg/kg. Mefloquine
(MEFL, 1379059 Sigma) was administrated once weekly by oral gavage at the
dosage of 50 mg/kg. Danusertib (A4116, ApexBio) were injected i.p. daily at
the dosage of 15 mg/kg. Arsenic trioxide (A1010, Sigma) was injected i.p.
daily at the dosage of 1.9 mg/kg (transferred from 0.15 mg/kg/day for
human). Everolimus (A8169, ApexBio) was administrated daily by oral gavage
at the dosage of 1 mg/kg. For IIA model, all drug treatments were
administrated from the day of injection till the end of experiment unless
specifically stated. For spontaneous model, treatments were administrated
after resection of primary tumors till the end of experiment.
Wang H., Tian L., Liu J., Goldstein A., Bado I., Zhang W., Arenkiel B.R., Li Z., Yang M., Du S., Zhao H., Rowley D.R., Wong S.T., Gugala Z, & Zhang X.H. (2018). The osteogenic niche is a calcium reservoir of bone micrometastases and confers unexpected therapeutic vulnerability. Cancer cell, 34(5), 823-839.e7.
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