Solvable 6ne9100

Mice were intravenously injected with an emulsion of TG-rich lipoprotein-like particles (80 nm) that were double-labelled with glycerol tri [³H]oleate and [¹⁴C]cholesteryl oleate (1 mg TG in 200 μL saline per mouse), prepared as described previously.²¹ Blood was collected at indicated time points to determine plasma decay of radiolabels. Tissues (approx. 50–200 mg) were dissolved in 0.5 mL Solvable (6NE9100, PerkinElmer, Waltham, Massachusetts, USA) at 56 °C overnight, after which 5.0 mL Ultima Gold (6013329, PerkinElmer, Waltham, Massachusetts, USA) was added. Plasma (4 μL) was directly added to 2.5 mL Ultima Gold. ³H- and ¹⁴C-activity was measured with a liquid scintillation counter (Tri-Carb 2910 TR, PerkinElmer, Waltham, Massachusetts, USA) and expressed as a percentage of injected dose per whole tissue or as a percentage of injected dose in plasma. One mouse was excluded for calculating the AUC (between 2 and 15 min after injection) of plasma decay since one plasma sample was missing. This criterium was set a priori since a sample from each time point is required for the AUC calculation.

Mice received an intravenous injection with an emulsion of TRL-like particles (80 nm) containing glycerol tri[³H]oleate (1 mg TG in 200 μL saline per mouse), prepared as described previously [49 (link)]. In experiment 1, 2-[1-¹⁴C]-deoxyglucose (NEC495A250UC; PerkinElmer, Waltham, MA, USA) was added to the emulsion to assess uptake of glucose and in experiment 2, TRL-like particles were double-labelled with [¹⁴C]cholesteryl oleate in addition to glycerol tri[³H]oleate to allow for TRL-remnant clearance. Blood was collected to determine plasma decay of radiolabels. After 15 minutes, mice were killed by CO₂ inhalation and perfused via the heart with ice-cold PBS. Various organs were collected and dissolved in 0.5 mL Solvable (6NE9100, PerkinElmer, Waltham, MA, USA) at 56°C overnight, after which 5.0 mL Ultima Gold (6013329, PerkinElmer, Waltham, MA, USA) was added. Plasma was directly added to 2.5 mL Ultima Gold. ³H-activity and ¹⁴C-activity were measured with a scintillation counter (Tri-Carb 2910 TR, PerkinElmer, Waltham, MA, USA) and expressed as a percentage of injected dose per gram tissue or as a percentage of injected dose in plasma. Mice were excluded from the calculation of the AUC (between 2 and 15 minutes after injection) of plasma decay if one or more plasma samples were missing.