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Antimouse horseradish peroxidase conjugated secondary antibody

Manufactured by Merck Group
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The Antimouse horseradish peroxidase-conjugated secondary antibody is a laboratory reagent used in various immunoassay techniques. It is composed of an antibody that specifically binds to mouse primary antibodies, conjugated to the enzyme horseradish peroxidase. This conjugate can be used to detect and quantify the presence of mouse antigens in biological samples.

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Lab products found in correlation

Anti rabbit peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Rabbit anti nav1, by Alomone (1 mentions) Mouse anti β actin, by Merck Group (1 mentions)

Spelling variants of this product

Horseradish peroxidase (696 citations) Horseradish peroxidase-conjugated secondary antibodies (350 citations) Peroxidase-conjugated secondary antibody (96 citations)

2 protocols using antimouse horseradish peroxidase conjugated secondary antibody

1

Western Blot Analysis of CBS and Nav1.7

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Expressions of CBS and Nav1.7 in T13-L2 DRGs from PMS and CON rats were determined using Western Blot analysis, as previously described in detail.27 (link) The primary antibodies including rabbit anti-CBS (1:1000, Abnova, USA), rabbit anti-Nav1.7 (1:1000, Alomone, USA), or mouse anti-β-actin (1:1000, Sigma, USA) and secondary antibodies including antirabbit peroxidase-conjugated secondary antibody (1:2000; Santa Cruz Biotechnology, CA) and antimouse horseradish peroxidase-conjugated secondary antibody (1:4000, Sigma, USA) were used to probe the target proteins.
Wang H.J., Xu X., Xie R.H., Rui Y.Y., Zhang P.A., Zhu X.J, & Xu G.Y. (2018). Prenatal maternal stress induces visceral hypersensitivity of adult rat offspring through activation of cystathionine-β-synthase signaling in primary sensory neurons. Molecular Pain, 14, 1744806918777406.
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2

Immunoblotting of Stat1 Phosphorylation

Cited 1 time
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Cell lysate preparation, gel electrophoresis, and blocking and staining protocols were performed as previously described. [27 (link)] Membranes were stained with primary antibody over-night at 4 °C for approximately 18 h. Anti-Stat1 and anti-pStat1 antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA and anti-mouse horseradish peroxidase-conjugated secondary antibody was purchased from Sigma (St. Louis, MO, USA).
Knitz M.W., Darragh L.B., Bickett T.E., Bhatia S., Bukkapatnam S., Gadwa J., Piper M., Corbo S., Nguyen D., Van Court B., Oweida A, & Karam S.D. (2021). Loss of cancer cell STAT1 improves response to radiation therapy and promotes T cell activation in head and neck squamous cell carcinoma. Cancer immunology, immunotherapy : CII, 71(5), 1049-1061.
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