Vecta elite kit

Manufactured by Vector Laboratories

Sourced in United States

The Vecta Elite kit is a laboratory equipment product offered by Vector Laboratories. It serves as a core component for various scientific applications. The kit provides essential tools and materials to facilitate research and experimentation.

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Lab products found in correlation

Axiocam mrc digital camera, by Zeiss (1 mentions) Axio scope a1, by Zeiss (1 mentions) Mouse and rabbit specific hrp dab abc detection ihc kit, by Abcam (1 mentions) Probe on superfrost plus slides, by Thermo Fisher Scientific (1 mentions)

2 protocols using vecta elite kit

Immunohistochemical Analysis of Tumor Proliferation

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Immunohistochemical analysis was performed on a thick (5 μm), paraformaldehyde-fixed, paraffin-embedded tumor tissue sections using the Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC Kit (Abcam Inc., Cambridge, United Kingdom) as reported precedingly (Wang et al., 2012 (link)). Briefly, tissue sections were immunostained with an anti-Ki-67 (1:50) or anti-PCNA (dilution 1:200). The number of Ki-67 or PCNA positive cells was determined in 10 randomly selected microscopic fields at ×400 magnification. The proliferation index of Ki-67 or PCNA was calculated as: the number of Ki-67 or PCNA positive cells/the total cell count × 100%. Immunofluore analyses for Ki67 (dilution 1:50) and PCNA (dilution 1:200) were carried out with the Vecta Elite kit (Vector Laboratories, Burlingame, CA, United States) based on the manufacturer’s manual. Sample analysis and image acquisition were achieved by Axio Scope A1 fluorescence microscope which was equipped with AxioCam MRC digital camera (Carl Zeiss, Oberkochen, Germany).

Lai Z.Q., Ip S.P., Liao H.J., Lu Z., Xie J.H., Su Z.R., Chen Y.L., Xian Y.F., Leung P.S, & Lin Z.X. (2017). Brucein D, a Naturally Occurring Tetracyclic Triterpene Quassinoid, Induces Apoptosis in Pancreatic Cancer through ROS-Associated PI3K/Akt Signaling Pathway. Frontiers in Pharmacology, 8, 936.

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Histological and Immunohistochemical Analysis

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Five micron sections were applied to Probe-on Superfrost Plus slides (Fisher Scientific, Pittsburgh, PA). Slides were stained with hematoxylin and eosin, and images were captured on a Zeiss microscope with a Zeiss Hrc5 camera (Carl Zeiss Microscopy, Thornwood, NY).
Immunohistochemistry was performed with the Vecta Elite kit (Vector Laboratories, Burlingame, CA) following the manufacturer’s protocol using their reagents. Antigen retrieval was performed by heating paraffin sections in a pressure cooker for 12 minutes followed by a one-hour incubation in the pressure cooker. Primary antibody was incubated overnight at 4°C and secondary antibody for 30 minutes at 37°C. Then, the signal was subsequently developed using athe DAB substrate kit for peroxidase. Quantification of proliferation was done by calculating the ratio of Ki67-positive cells to the number of cells in the basal layer.

Le Bras G.F., Taylor C., Koumangoye R.B., Revetta F., Loomans H.A, & Andl C.D. (2014). TGFβ loss activates ADAMTS-1-mediated EGF-dependent invasion in a model of esophageal cell invasion. Experimental cell research, 330(1), 29-42.

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