Fluconazole flc

Ascorbic acid (AA; Fisher Scientific, Cat No. A61–25, CAS 5081–7, Waltham, MA, USA) was prepared from a stock of 1 M and used at 10 mM. A reduced form of glutathione (GSH; Alfa Aesar, Cat No. AAJ6216606, CAS 70–18–8, Ward Hill, MA, USA) was prepared from a stock of 0.5 M and used at 10 mM. Pyrrolidine dithiocarbamate (PDTC; Cayman Chemicals, Cat No. 20713, CAS 5108–96–3, Ann Arbor, MI, USA) was prepared from a stock of 10 mM and used at 10 μM. Retinoic acid (RA; Cayman Chemical, Cat No. 11017, CAS 302–79–4) was prepared from a stock of 100 mM (dissolved in dimethyl sulfoxide (DMSO)) and used at 1 mM. Fluconazole (FLC; Cayman Chemical, Cat No. 11594, CAS 86386–73–4) was dissolved in DMSO as a 50 mg/mL stock and used at 32 μg/mL. Amphotericin B (AMB; Fisher Scientific, Cat No. BP 928 250) was prepared as a stock of 2 mg/mL (dissolved in dimethyl sulfoxide (DMSO)) and used at 2 μg/mL.

The Screen-Well FDA-approved drug library was purchased from Enzo Life Sciences (catalogue no. BML-2841; Farmingdale, NY, USA). The 640 compounds in this library were provided at 2 mg/mL in dimethyl sulfoxide (DMSO). Flubendazole (FLB), mebendazole (MEB), benomyl (BEN), nisoldipine (NIS), nifedipine (NIF), felodipine (FEL), niguldipine (NIG), amphotericin B (AMB), itraconazole (ITZ), voriconazole (VRZ) and 5-flucytosine (5FC) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fluconazole (FLC) was obtained from Cayman Chemical (Ann Arbor, MI, USA). Stock solutions of amphotericin B, itraconazole and voriconazole were prepared at 1.6 mg/mL in water; 5-flucytosine was made at 6.4 mg/mL in water; fluconazole was made at 12.8 mg/mL in water and all remaining drugs were dissolved in DMSO at 2 mg/mL. All compounds were stored at −80 °C.