Nod cb17 prkdcscidil2rgtm1wjl szj

Manufactured by Jackson ImmunoResearch

NOD.CB17-PrkdcscidIl2rgtm1Wjl/SzJ is a laboratory mouse strain. It is a model of severe combined immunodeficiency (SCID) with a deficiency in the interleukin-2 receptor gamma chain.

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Lab products found in correlation

Ami htx, by Spectral Instruments Imaging (1 mentions)

2 protocols using nod cb17 prkdcscidil2rgtm1wjl szj

Renal Cell Carcinoma Tumor Metabolism Study

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All mouse infusions were performed in compliance with protocols approved by the Institutional Animal Care and Use Committee at the University of Texas Southwestern Medical Center (Protocol 2016–101694). NOD.CB17-Prkdc^scidIl2rg^tm1Wjl/SzJ (NSG) male and female mice aged 4–8 weeks old were purchased from the Jackson Laboratory. All mice were housed in the Animal Resource Center at the University of Texas Southwestern Medical Center under a 12 hr light-dark cycle and fed ad libitum.
Human subjects research was approved by the Institutional Review Board of UT Southwestern Medical Center. After obtaining informed consent, patients were enrolled on protocol STU062010–157 (Clinicaltrials.gov protocol NCT01888082): An Investigation of Tumor Metabolism in Patients Undergoing Surgical Resection. Patients were selected for inclusion based on imaging and clinical features consistent with renal cell carcinoma.

Cai F., Bezwada D., Cai L., Mahar R., Wu Z., Chang M.C., Pachnis P., Yang C., Kelekar S., Gu W., Brooks B., Ko B., Vu H.S., Mathews T.P., Zacharias L.G., Martin-Sandoval M., Do D., Oaxaca K.C., Jin E.S., Margulis V., Malloy C.R., Merritt M.E, & DeBerardinis R.J. (2023). Comprehensive isotopomer analysis of glutamate and aspartate in small tissue samples. Cell metabolism, 35(10), 1830-1843.e5.

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Orthotopic and Metastasis Assays in Mice

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All procedures were performed in strict accordance with the Guide for Care and Use of Laboratory Animals approved by the UT Southwestern Institutional Animal Care and Use Committee (IACUC). Healthy Hsd:Athymic Nude-Foxn1nu (Envigo) and NOD.CB17-Prkdcscid Il2rgtm1Wjl/SzJ (Jackson) female mice from 4-6 weeks of age were used for studies. All mice were housed in pathogen free conditions. For orthotopic kidney injection, luciferase labelled (5.0 x 105) cells were resuspended in matrigel (20μl) and inoculated under left renal capsule. For experimental metastasis assay, luciferase labelled (2.0 x 105) cells were resuspended in PBS (100μl) and intracardially injected into the right ventricle of mice. Tumors were detected by non-invasive bioluminescence imaging of experimental animals using an AMI-HTX (Spectral Instruments Imaging). Bioluminescence images were quantified using ROI tool in Aura software.

Kim K., Huang H., Parida P.K., He L., Marquez-Palencia M., Resse T.C., Kapur P., Brugarolas J., Brekken R.A, & Malladi S. (2023). Cell Competition Shapes Metastatic Latency and Relapse. Cancer discovery, 13(1), 85-97.

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