Asic3

DMEM/F12, fetal bovine serum, and B27 supplement were purchased from Gibco Invitrogen Corporation (Carlsbad, CA, USA). Hoechst 33258, trypsin, iberiotoxin (IbTX), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and amiloride were obtained from Sigma-Aldrich (St. Louis, MO, USA). The reagent kit for determining lactate dehydrogenase (LDH) was purchased from Nanjing Jiancheng Institute of Biological Engineering (Nanjing, China). Psalmotoxin 1(PcTX1) and primary antibodies of ASIC1, ASIC2, and ASIC3 were purchased from Alomone Labs (Jerusalem, Israel). Additional files showed the specificity of PcTX1 and ASICs antibodies (see Additional files 1 and 2). Potassium-binding benzofuran isophthalate acetoxymethyl ester (PBFI-AM) and pluronic F-127 were obtained from Molecular Probes Inc. (Eugene, OR, USA). Primary antibodies of Bax and Bcl-2 were purchased from Cell Signaling Technology Inc. (San Francisco, CA, USA). Anti-NLRP1 and anti-BK antibody were purchased from Abcam (San Francisco, CA, USA). Primary antibodies of caspase-1, activated-caspase-3, ASC, IL-1β, and IL-18 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Horseradish peroxidase-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Other general agents were commercially available.