Xtt assay kit

Cell viability was measured using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay kit (Biotium) using our previously established protocol.^{45 (link)} Briefly, experiments were carried out in human neuroblastoma SH-SY5Y cell lines (ATCC) grown in DMEM and Ham’s F12K (1:1) medium containing 10% FBS and 1% penicillin/streptomycin. Cells were maintained at incubator conditions set to a temperature of 37 °C and 5.5% CO₂. The cells were seeded at a density of 30 000 per well in a clear bottom 96-well plate 24 h prior to oligomers incubation. Oligomers were incubated at 2.5 μM concentration for 24 h prior to performing XTT assay. All experiments were done in triplicates, and statistical analysis and data processing were carried out using Origin 8.0.

Cell viability was measured using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]- 2H- tetrazolium hydroxide (XTT) assay kit (Biotium) following the previously established protocol with few modifications [74 (link)]. Briefly, human neuroblastoma SH-SY5Y cells (ATCC, Manassas, VA) were maintained in a humidified incubator at 37 °C with 5.5% CO₂ in 1:1 mixture of DMEM and Ham’s F12K medium with 10% FBS and 1% penicillin/streptomycin. Approximately, 15,000 cells were plated in a clear bottom 96 well black plates (Thermo Scientific) 24 hours prior to sample treatment. All the reaction samples were prepared and purified using autoclaved water and buffer to avoid bacterial contamination. Cell medium from wells was replaced with the reaction samples resuspended in complete growth medium and incubated for 24 hours prior to XTT assay.