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Rneasy mini kit rna cleanup procedure

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The RNeasy mini kit is a laboratory equipment product used for RNA cleanup and purification. The kit utilizes a silica-membrane-based technology to efficiently capture and purify RNA molecules from a variety of sample types. The core function of the RNeasy mini kit is to provide a reliable and reproducible method for isolating high-quality RNA for downstream applications such as gene expression analysis, RT-PCR, and NGS.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (3 mentions) Cfx96 apparatus, by Bio-Rad (3 mentions) Iscript one step rt pcr kit, by Bio-Rad (2 mentions) Sybr green, by Bio-Rad (1 mentions) Iscript one step rt pcr kit with sybr green, by Bio-Rad (1 mentions)

Close spelling variants of this product

RNeasy Mini Cleanup Kit RNeasy Mini Procedure RNA mini cleanup kit RNeasy Mini cleanup RNeasy cleanup kit RNeasy procedure RNA cleanup kit RNeasy Mini Kit RNeasy kit Mini Kit RNA cleanup

3 protocols using rneasy mini kit rna cleanup procedure

1

Tissue-Specific RNA Isolation and qRT-PCR Analysis

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Total RNAs were isolated from liver, lung, spleen, abdominal fat tissue, and colon using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. Messenger RNAs (mRNAs) were purified using the RNeasy mini kit RNA cleanup procedure (Qiagen, Valenica, CA). Quantitative reverse-transcription polymerase chain reactions (RT-PCRs) were performed using the iScript One-Step RT-PCR Kit with SYBR Green (Bio-Rad, Hercules, CA) in a CFX96 apparatus (Bio-Rad) with specific mouse oligonucleotides (Table 1). Results were normalized to the 36B4 (housekeeping gene).
Etienne-Mesmin L., Vijay-Kumar M., Gewirtz A.T, & Chassaing B. (2016). Hepatocyte Toll-Like Receptor 5 Promotes Bacterial Clearance and Protects Mice Against High-Fat Diet–Induced Liver Disease. Cellular and Molecular Gastroenterology and Hepatology, 2(5), 584-604.
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2

Quantitative RT-PCR Analysis of Mouse Tissues

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Total RNAs were isolated from liver, lung, spleen, abdominal fat tissue and colon using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. mRNAs were purified using RNeasy mini kit RNA cleanup procedure (Qiagen). Quantitative RT-PCRs were performed using the iScriptTM One-Step RT-PCR Kit with SYBR Green (Bio-Rad, Hercules, CA) in a CFX96 apparatus (Bio-Rad, Hercules, CA) with specific mouse oligonucleotides (Table 1). Results were normalized to the 36B4 (housekeeping gene).
Etienne-Mesmin L., Vijay-Kumar M., Gewirtz A.T, & Chassaing B. (2016). Hepatocyte Toll-Like Receptor 5 Promotes Bacterial Clearance and Protects Mice Against High-Fat Diet–Induced Liver Disease. Cellular and Molecular Gastroenterology and Hepatology, 2(5), 584-604.
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3

Tissue-Specific Gene Expression Analysis

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Following euthanasia, colon, liver and epididymal fat were collected. Total RNA was isolated from colon, liver and epididymal fat using TRIzol (Invitrogen, Carlsbad, CA) according to its provided instructions, mRNAs of liver and fat were further purified using the RNeasy mini kit RNA cleanup procedure (Qiagen, Valenica, CA). The expression level of IL-6, TNF-α and MCP-1 in liver and fat, claudin-1 in colon was analyzed by using quantitative RT-PCR (qRT-PCR) according to the Biorad iScript™ One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the primers listed in the key resources table. Difference in transcript levels were quantified by normalization of each amplicon to housekeeping gene 36B4.
Zou J., Ngo V.L., Wang Y., Wang Y, & Gewirtz A.T. (2022). Maternal fiber deprivation alters microbiota in offspring resulting in low grade inflammation and predisposition to obesity. Cell host & microbe, 31(1), 45-57.e7.
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