Snl 10 tips

Manufactured by Bruker

The SNL-10 tips are a set of specialized laboratory equipment designed for precise liquid handling tasks. The tips are compatible with various pipettes and dispensers, facilitating accurate and reproducible liquid transfers. The core function of the SNL-10 tips is to provide a reliable and consistent interface for the introduction and extraction of liquids in laboratory experiments and procedures.

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Lab products found in correlation

Peakforce qnm, by Bruker (1 mentions) Multimode nanoscope 8 instrument, by Bruker (1 mentions) Ptc 200 peltier thermal cycler, by Bio-Rad (1 mentions) Nanoscope multimode 8, by Bruker (1 mentions) Multimode 5 atomic force microscope, by Veeco (1 mentions) Scanasyst fluid tip, by Bruker (1 mentions) Multimodetm 8 microscope, by Bruker (1 mentions)

6 protocols using snl 10 tips

Nanomechanical Characterization of Origami and Metal Patterns

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All atomic force microscopy (AFM) images were obtained using a Multimode Nanoscope VIII instrument (Bruker) under tapping mode in fluid with SNL-10 tips (Bruker).
The nanomechanical properties were measured with the Multimode Nanoscope VIII instrument (Bruker) in the “PeakForce QNM in Fluid” mode. “SCANASYST-FLUID+” model cantilever with nominal spring constants of ~0.7 N m⁻¹ and tip radius of ~2 nm was chosen (Bruker) for the measurements. In FD-based AFM, the AFM probe was made to approach to and retract from samples to record a series of FD curves. The “NanoScope Analysis” software was used to process the FD curves. The statistical analysis of the Young’s modulus distribution was evaluated with about 25 FD curves of origami and Cu metallization pattern, and fitted with a Gaussian model.

Jia S., Wang J., Xie M., Sun J., Liu H., Zhang Y., Chao J., Li J., Wang L., Lin J., Gothelf K.V, & Fan C. (2019). Programming DNA origami patterning with non-canonical DNA-based metallization reactions. Nature Communications, 10, 5597.

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DNA Origami Nanostructure Characterization

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For preparing DNA origami short DNA staple strands and the M13mp18 scaffold strand were mixed in 1 × TAE-Mg buffer with the final concentrations of 100 and 5 nM, respectively. The mixture was annealed from 95 to 5 °C (1 °C min⁻¹) using a PTC-200 Peltier Thermal Cycler (MJ Research)28 (link). Directly deposit the DNA origami solution (5 μl) on a freshly cleaved mica surface and leave it to absorb for 5 min. The mica surface was washed with 1 × TAE-Mg for two times and incubated with a solution of the translated result at RT for 30 min. AFM was used to display the calculated results on DNA origami through scanning with a Bruker Multimode Nanoscope VIII instrument under tapping mode in fluid with SNL-10 tips (Bruker).

Liu H., Wang J., Song S., Fan C, & Gothelf K.V. (2015). A DNA-based system for selecting and displaying the combined result of two input variables. Nature Communications, 6, 10089.

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Origami-Enzyme and TDN-Enzyme Conjugations

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For the characterization of origami-enzyme conjugations, 3 µL of sample was deposited onto a freshly cleaves mica surface and allowed to adsorb for 3 min. We added 300 µL of 1 × TAE-Mg²⁺ buffer to the liquid cell and the characterization was conducted using SNL-10 Tips (Bruker) by Multimode Nanoscope VII (Bruker) instrument in tapping mode.
For the characterization of TDN-enzyme conjugation, gold surface of electrochemical chip was cleaned with isopropanol by sonication for 2 min, and then cleaned in water for 20 min by sonication. The TDN was immobilized with the same method for electrochemical detection. The AFM characterization were conducted in peakforce mode.

Song P., Shen J., Ye D., Dong B., Wang F., Pei H., Wang J., Shi J., Wang L., Xue W., Huang Y., Huang G., Zuo X, & Fan C. (2020). Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework. Nature Communications, 11, 838.

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Atomic Force Microscopy of 2D DNA Nanostructures

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Images of folded structures were obtained with a Veeco Multimode V atomic force microscope. C-type Bruker SNL-10 tips were used under tapping mode in fluid. Samples (25 μl) were deposited on the mica surface for 1 min. The mica surface was then rinsed five times with 0.5 × TE (5 mM Tris, 1 mM EDTA, adjusted to pH 8.0) supplemented with 25 mM MgCl₂. For the 2D DNA brick shapes, samples were supplemented with 5 mM NiCl₂ (final concentration) to aid in attachment to the mica surface before imaging. The 2D origami rectangle was imaged in 1 × TE (10 mM Tris, 1 mM EDTA, adjusted to pH 8.0) supplemented with 25 mM MgCl₂.

Myhrvold C., Baym M., Hanikel N., Ong L.L., Gootenberg J.S, & Yin P. (2017). Barcode extension for analysis and reconstruction of structures. Nature Communications, 8, 14698.

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Atomic Force Microscopy of Micelle Structures

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An
MFP-3D stand-alone
AFM (Asylum Research, Oxford Instruments, Santa Barbara, CA) was used
for imaging the structures of micelles on the mica surfaces. Scanning
in noncontact (tapping) mode under aqueous solution was carried out
using SNL-10 tips (silicon tip on a silicon nitride cantilever, Bruker)
with a nominal spring constant of 0.35 N/m. The AFM tip holder was
irradiated in a UV-ozone cleaning system (ProCleaner Plus, BioForce
Nanosciences, United States) for 15 min prior to use.

Lin W., Kampf N, & Klein J. (2020). Designer Nanoparticles as Robust Superlubrication Vectors. ACS Nano, 14(6), 7008-7017.

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Oligonucleotide-Functionalized CNT Characterization

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The morphology of oligonucleotide P2-functionalized CNT (CNT-P) was observed by AFM. Briefly, the sample was diluted up to 25-fold in TE-Mg²⁺ (20 mM Tris base, 1 mM EDTA, 12.5 mM MgCl₂, pH 7.4). Five microliters of the resulting solution was deposited onto a freshly cleaved mica surface (Plano GmbH) and adsorbed for 3 min at room temperature. After addition of 10 µL TAE-Mg²⁺ (40 mM Tris, 20 mM acetic acid, 2 mM EDTA, 12.5 mM Mg acetate, pH 8.0), the sample was scanned with sharpened pyramidal tips (SNL-10 tips, radius 2 nm, spring constant 0.35 N m⁻¹, Bruker) using a MultiModeTM 8 microscope (Bruker) equipped with a Nanoscope V controller in Tapping Mode.
To analyze the morphology and roughness of SiNP/CNT-DNA nanocomposite surfaces, the fresh materials were rinsed with distilled water, transferred to the petri dish and dried as described above. The dried samples were immersed in water and imaged with pyramidal tips (ScanAsyst Fluid tips, radius 20 nm, spring constant 0.7 N m⁻¹, Bruker) using a NanoWizard 3 atomic force microscope (JPK) under a force-curve based imaging mode (QI^TM). Images were acquired with 256 × 256 pixel resolution and the roughness (Ra) of the surfaces was extracted using the JPK data processing software (version spm-6.0.74). PLL, PLL + G, Matrigel, and Geltrex control surfaces were characterized in the same way.

Hu Y., Domínguez C.M., Bauer J., Weigel S., Schipperges A., Oelschlaeger C., Willenbacher N., Keppler S., Bastmeyer M., Heißler S., Wöll C., Scharnweber T., Rabe K.S, & Niemeyer C.M. (2019). Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings. Nature Communications, 10, 5522.

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