Mhcd4528

Manufactured by BioLegend

MHCD4528 is a laboratory instrument designed for the detection and analysis of biological samples. It features advanced optical and electronic components to enable precise measurements and data collection.

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Lab products found in correlation

Murine cd45, by BioLegend (1 mentions) Apc annexin 5 apoptosis detection kit, by BioLegend (1 mentions) Flojo software, by Tree Star (1 mentions) Facsaria 2, by BD (1 mentions)

2 protocols using mhcd4528

Xenograft Tumor Growth Monitoring

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All in vivo studies were conducted under the auspices of protocols approved by the Dana-Farber Cancer Institute Animal Care and Use Committee. Six- to eight-week old female NSG-mice or C57BL/6-mice (The Jackson Laboratory) were injected with 1×10⁶ cells. Bone marrow was harvested from femur, tibia and spine, and red blood cells were lysed (Sigma) prior to staining with human CD45 (Invitrogen Cat. No. MHCD4528) and murine CD45 (Biolegend Cat. No. 103113) antibodies and analysis by flow cytometry.

Cremer A., Ellegast J.M., Alexe G., Frank E.S., Ross L., Chu S.H., Pikman Y., Robichaud A., Goodale A., Häupl B., Mohr S., Rao A.V., Walker A.R., Blachly J.S., Piccioni F., Armstrong S.A., Byrd J.C., Oellerich T, & Stegmaier K. (2019). Resistance mechanisms to SYK inhibition in acute myeloid leukemia. Cancer discovery, 10(2), 214-231.

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Apoptosis Analysis by Flow Cytometry

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Apoptosis was assessed with an APC Annexin V Apoptosis Detection Kit (BioLegend) following the manufacturer’s protocol. Mouse samples from bone marrow and peripheral blood were stained with human CD45 (Invitrogen Cat. No. MHCD4528) and murine CD45 (Biolegend Cat. No. 103113) antibodies, unless the cells had been previously infected with a fluorescence marker (GFP, mcherry or mAmetrine). Flow cytometry was performed on a LSR Fortessa or FACS Celesta flow cytometer. Cells were sorted on a FACSAria II flow cytometer (BD Biosciences). The data were analyzed with FloJo software (TreeStar).

Ellegast J.M., Alexe G., Hamze A., Lin S., Uckelmann H.J., Rauch P.J., Pimkin M., Ross L.S., Dharia N.V., Robichaud A.L., Conway A.S., Khalid D., Perry J.A., Wunderlich M., Benajiba L., Pikman Y., Nabet B., Gray N.S., Orkin S.H, & Stegmaier K. (2022). Unleashing cell-intrinsic inflammation as a strategy to kill AML blasts. Cancer discovery, 12(7), 1760-1781.

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