3 v2 reagent kit

Manufactured by 10x Genomics

Sourced in United States

The 3' v2 Reagent Kit is a laboratory instrument designed for use with 10x Genomics' Chromium platform. The kit contains the necessary reagents and consumables for performing single-cell RNA sequencing experiments using the 3' gene expression chemistry.

Automatically generated - may contain errors

Lab products found in correlation

Chromium single cell platform, by 10x Genomics (2 mentions) Chromium single cell controller, by 10x Genomics (1 mentions)

Spelling variants of this product

Chromium Single Cell 3′ Reagent Kits v2 (47 citations) Single Cell 3′ Reagent Kits v2 (22 citations) Chromium Single Cell 3′ Reagent Kits v2 Chemistry (3 citations) Single Cell 3' Reagent Kits v2 User Guide (2 citations) Chromium Single Cell 3’ Reagent Kits V2 and V3 (2 citations)

3 protocols using 3 v2 reagent kit

Single-cell cDNA sequencing using Chromium

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Single-cell cDNA libraries were generated using the Chromium single-cell platform and the 3′ v2 Reagent Kit according to the manufacturer’s protocol (10× Genomics, USA). Single-cell libraries were sequenced on a NextSeq 500 platform (3 samples (whole single cells) and 2 samples (single nuclei) on one flow cell) to obtain 100- and 32-bp paired end reads using the following read length: read 1, 26 cycles, read 2, 98 cycles and i7 index, 8 cycles.

Bentsen M.A., Rausch D.M., Mirzadeh Z., Muta K., Scarlett J.M., Brown J.M., Herranz-Pérez V., Baquero A.F., Thompson J., Alonge K.M., Faber C.L., Kaiyala K.J., Bennett C., Pyke C., Ratner C., Egerod K.L., Holst B., Meek T.H., Kutlu B., Zhang Y., Sparso T., Grove K.L., Morton G.J., Kornum B.R., García-Verdugo J.M., Secher A., Jorgensen R., Schwartz M.W, & Pers T.H. (2020). Transcriptomic analysis links diverse hypothalamic cell types to fibroblast growth factor 1-induced sustained diabetes remission. Nature Communications, 11, 4458.

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Single-cell RNA-seq analysis pipeline

Cited 1 time

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Single-cell cDNA libraries were generated using the Chromium Single Cell platform and 3′ v2 Reagent Kit according to manufacturer’s protocol (10x Genomics). Single-cell libraries were sequenced on a NextSeq 500 platform to obtain 100-bp and 32-bp paired-end reads using the following read length: read 1, 26 cycles; read 2, 98 cycles; and i7 index, 8 cycles. Cell Ranger^{51 (link)} (version 2.0.1) was used with default parameters to demultiplex and align reads to the hg19 reference genome, filter cell and unique molecular identifier (UMI) barcodes and generate gene count matrices.

Palani N.P., Horvath C., Timshel P.N., Folkertsma P., Grønning A.G., Henriksen T.I., Peijs L., Jensen V.H., Sun W., Jespersen N.Z., Wolfrum C., Pers T.H., Nielsen S, & Scheele C. (2023). Adipogenic and SWAT cells separate from a common progenitor in human brown and white adipose depots. Nature Metabolism, 5(6), 996-1013.

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Single-cell RNA-seq Library Generation

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Single-cell cDNA libraries were generated using the 10x Genomics (USA) Chromium single-cell controller and the 3′ v2 Reagent Kit according to manufacturer's protocol. Single-cell libraries were sequenced on a NextSeq 500 platform with 3 samples on one flow cell to obtain 100 and 32-bp paired end reads using the following read length: read 1, 26 cycles, read 2, 98 cycles and i7 index, 8 cycles.

Brown J.M., Bentsen M.A., Rausch D.M., Phan B.A., Wieck D., Wasanwala H., Matsen M.E., Acharya N., Richardson N.E., Zhao X., Zhai P., Secher A., Morton G.J., Pers T.H., Schwartz M.W, & Scarlett J.M. (2021). Role of hypothalamic MAPK/ERK signaling and central action of FGF1 in diabetes remission. iScience, 24(9), 102944.

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