We designed two types of fusion genes: one consisting of the chromogranin A promoter and CreERT2 (CgA-CreERT2), and the other consisting of the CAG-promoter [16 (link)], the floxed-stop sequence, and the SV40 T-antigen (CAG-LSL-SV40Tag) (Fig 1a and 1b ). The purified fragments (10 μg/ml) were microinjected into the pronuclei of fertilized C57/B6N mouse (SLC, Shizuoka, Japan) eggs. Viable eggs were transferred into the oviducts of pseudopregnant female ICR mice (SLC) using standard techniques. Transgenic founder mice were identified by PCR. For experiments, we used heterozygous transgenic mice. Rosa-CAG-LSL-ZsGreen1 mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA). Animals were maintained in a specific pathogen free facility on a 12-h light/12-h dark cycle at 25°C with free access to water and standard diet (SD; CE-2, 352 kcal/100 g, CLEA Japan, Tokyo, Japan). Animals were euthanized by cervical dislocation. All experimental procedures were approved by the Kyoto University Graduate School of Medicine Committee on Animal Research.
Rosa cag lsl zsgreen1 mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
The Rosa-CAG-LSL-ZsGreen1 mice are a genetically engineered mouse line that expresses the ZsGreen1 fluorescent protein. The ZsGreen1 protein is a bright green fluorescent protein derived from a coral species. In these mice, the expression of ZsGreen1 is controlled by the CAG promoter and is dependent on the removal of a loxP-flanked STOP cassette. This allows for cell-type specific and inducible expression of the fluorescent protein.
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3 protocols using rosa cag lsl zsgreen1 mice
1
Transgenic Mice Generation Using Fusion Genes
2
Conditional BMPRII Knockout in Mouse DG
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All animal procedures were performed in accordance with the Public Health Service Policy on Humane Care and Use of Laboratory Animals and were approved by the Northwestern University Institutional Animal Care and Use Committee. Eight to ten week old C57Bl/6 male and female mice (Jackson Laboratory) were used for antidepressant treatment experiments as well as DG virus injection experiments. For antidepressant and virus injection experiments, animals were allocated to experimental groups by randomly assigning each cage to an experimental treatment. BMPRII floxed mice (BMPRIIfx/fx)22 (link) were mated to mice containing tamoxifen-inducible Cre recombinase under the control of the Ascl1 promoter (Ascl1-CreER™)23 (link). To monitor Cre expression, these mice were crossed with Rosa-CAG-LSL-ZsGreen1 mice (RosaZG/ZG, Jackson Laboratory)24 (link). Tamoxifen-induced ablation of BMPRII was performed at 8–10 weeks of age in BMPRIIfx/fx; Ascl1-CreER™; RosaZG/ZG mice. BMPRII+/+; Ascl1-CreER™; RosaZG/ZG littermates were used as controls. All mice were housed 3–5 per cage under a 14:10 hour light:dark cycle with ad libitum access to food and water.
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Conditional BMPRII Knockout in Mouse DG
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