Bradford reagent

Antibodies were obtained from the following sources; mouse anti-Nestin antibody (BD Transduction Laboratories), mouse anti-Synaptophysin (7H12, Cell Signaling Technology), rabbit anti-Map2 (D5G1, Cell Signaling Technology), anti-Cleaved Caspase-3 (Ab-2, Millipore-Sigma Aldrich), anti-PUMAα/β Antibody (G-3, Santa Cruz Biotechnology, Inc.), anti-Bcl-2 (C-2, Santa Cruz Biotechnology, Inc.), anti-Mcl-1 (S-19, Santa Cruz Biotechnology, Inc.), anti-Bax (N-20, Santa Cruz Biotechnology, Inc.), anti-Bad (Cell Signaling Technology), anti-SRSF1 (3G268, Santa Cruz Biotechnology, Inc.), anti-Tubulin (Millipore-Sigma-Aldrich). Mammalian protease inhibitors were obtained from Sigma-Aldrich (St Louis, MO). Bradford reagent was from BioWorld (Dublin, OH). MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) was obtained from Thermo Fisher Scientific (#M6494).

Antibodies were obtained from the following sources: anti-Nef (SF2), EH1 from NIH-AIDS Reagent Program, anti-GAPDH and anti-HSP-70 from Santa Cruz (Dallas, TX, USA), anti-tau from Santa Cruz and anti-PHF-tau from Pierce/Thermo Fisher, Inc., (Waltham, MA, USA), anti-beta tubulin class III from Sigma-Aldrich (St. Louis, MO, USA), anti-Alix Cell Signaling, Inc. (Beverly, MA, USA), LC3B from Sigma-Aldrich, β-tubulin from LI-COR, Odyssey (Lincoln, NE, USA), anti-neurofilament medium chain from Cell Signaling, Inc., anti-pAKT (Ser476), anti-AKT, p44/42 MAPK (Thr202/204), anti-MAPK, pmTOR (Ser2448), and anti-mTOR were purchased from Cell Signaling Technology (Danvers, MA, USA). Mammalian protease inhibitors were obtained from Sigma-Aldrich. Bradford reagent was from BioWorld (Dublin, OH, USA). GSH-Glo Glutathione Assay kit was purchased from Promega Corp. (Madison, WI, USA).