Ps1m146v

Breeding pairs of triple transgenic mice (3xTg), carrying the PS1M146V, APPSwe, and tauP301L transgenes (Oddo et al, 2003 (link)), were initially purchased from The Jackson Laboratories (JAX), United States of America. Animals used in this study were bred in the animal facility of the Institute of Molecular Biosciences, Graz, Austria, using the breeding pairs from Charles River Laboratories. The control strain used for the AD disease model was B6129SF2/J, also bred in‐house with breeding pairs from Charles River Laboratories. Briefly, animals were housed in groups of two to four animals per cage under specific pathogen‐free (SPF) conditions in a 14 h/10 h light/dark cycle with ad libitum access to standard chow (Ssniff, cat. #V1536) and autoclaved tap water. Autoclaved nest material and one paper house per cage served as cage enrichment. All animal experiments were performed in accordance with national and European ethical regulation (Directive 2010/63/EU) and approved by the responsible institutional or government agencies (Bundesministerium für Wissenschaft, Forschung und Wirtschaft, BMWFW, Austria: BMWFW‐66.007/0032‐V/3b/2019).

Mice were stably transfected with mutant human APPswe,TauP301L genes and mutant mice gene PS1M146V (called 3 × Transgene-AD mouse) were bought from the Jackson Laboratory (JAX order number 3591206, Bar Harbor, ME, USA). The 3 × Transgene-AD mice were bred at 12 h mild 12 h darkish stipulations of specific pathogen-free (SPF) circumstances. All animal experiments were conducted with the approval of the Laboratory Animal Ethics Committee of Shenzhen University (Permit Number:SYXK 2014-0140) and in accordance with the guidelines and regulations for animal experiments. The hippocampus of the new-born (within 24 h) 3 × Transgene-AD mice was used to obtain the primary neuron cells. The pre-cold HBSS was used to wash the dissected hippocampal tissues, then hippocampal tissues were cut into small portions using a surgical blade. These fragments were transferred to a new plate containing papain (2 mg/mL) and digested for 30 min at 37°C. The digested cells were filtrated and then centrifuged at 1,000 g for 5 min. After discarding the supernatant, the cell pellets were resuspended in medium and plated on poly-L-lysine (0.1 mg/mL)-coated plates. A neurobasal medium containing 2% B27 supplement, 1% L-glutamine, 100 unit/mL penicillin, and 100 μg/mL streptomycin was used to culture the primary neuron cells in a humidified 5% CO₂ environment at 37°C.

The triple transgenic AD model mice (3 × Tg-AD) carrying human gene mutants APPswe, PS1M146V, and tauP301L were purchased from the Jackson laboratory (Bar Harbor, ME, USA). Six male 3 ×Tg AD mice were selected for the experiments of MOST technology, among them three were 6-month-old (abbreviated as AD06) and another three were 12-month-old (AD12).
All the animals were housed in an environment with a temperature of 22  ± 1 °C, relative humidity of 50  ± 1% and a light/dark cycle of 12/12 hr. Additionally, all animal studies (including the mice euthanasia procedure) were done in compliance with the regulations and guidelines of Shenzhen University Institutional Animal Care Center, Experimental Animal Ethics Committee of Shenzhen University Medical Department and the AAALAC and the IACUC guidelines (animal experiment proof certificate number: SYXK2014-0140).