For cryoinjury, mice were anesthetized, and dry ice was applied directly to the exposed tibialis anterior (TA) and gastrocnemius muscles for 5 s. The skin incision was closed with 4–0 Prolene suture (Ethicon Inc., Somerville, NJ, USA) immediately after injury. This procedure generates a reproducible injury in the muscle with a discrete border between uninjured and injured muscle (28 (link), 29 (link)). Injured muscles were allowed to recover for 5 days prior to mouse euthanasia and muscle harvest. For quantification of regenerating myofiber size after cryoinjury, a series of images were taken spanning the entire regenerating area in cross section (CSA); the sizes of 10 regenerating myofibers (identified by their centrally located nuclei) were measured in each image using ImageJ software (RRID: SCR_003070), which collectively resulted in a total of approximately 100 myofiber sizes measured for each animal.
4 0 prolene suture
Manufactured by Johnson & Johnson
Sourced in United States
The 4-0 prolene suture is a sterile, non-absorbable surgical suture made of polypropylene. It is commonly used in a variety of surgical procedures to close wounds and approximate tissue.
Automatically generated - may contain errors
Lab products found in correlation
Automatic injector, by Stoelting (2 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
100n load cell, by Instron (1 mentions)
6 0 prolene suture, by Johnson & Johnson (1 mentions)
Xylazine, by Bayer (1 mentions)
Zoletil, by Virbac (1 mentions)
C57bl 6 mice, by Koatech (1 mentions)
Octopus tissue stabilizer, by Medtronic (1 mentions)
10 protocols using 4 0 prolene suture
1
Cryoinjury-Induced Muscle Regeneration in Mice
2
Stereotactic Injection of Lentiviral Vectors in Mice
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Thirteen adult 20 g male C57/BL6 mice were used (Iffa Credo/ Charles River, Les Oncins, France). The animals were housed in a temperature-controlled room and maintained on a 12 h day/night cycle. Food and water were available ad libitum. The experiments were performed in accordance with the European Community directive (86/609/EEC) for the care and use of laboratory animals.
Concentrated viral stocks were thawed on ice and resuspended by repeated pipetting. The mice were anaesthetized using 75 mg/kg ketamine and 10 mg/kg xylazine, administered intraperitoneally. Lentiviral vectors were stereotaxically injected into the striatum using a 34-gauge blunt-tip needle linked to a Hamilton syringe (Hamilton, Reno, NV, USA) by a polyethylene catheter at the following stereotaxic coordinates: 0.5 mm rostral to bregma, 2 mm lateral to midline and 3.5 mm from the skull surface. Each mouse received the vector encoding mHtt alone in the left striatum and the vectors encoding both mHtt and AMPKγGOF in the right striatum.
Two hundred nanograms of p24 antigen of each viral vector was injected at 0.2 µl/min by means of an automatic injector (Stoelting Co., Wood Dale, USA) and the needle was left in place for an additional 5 min. The skin was closed using 4-0 Prolene suture (Ethicon, Johnson and Johnson, Brussels, Belgium).
Concentrated viral stocks were thawed on ice and resuspended by repeated pipetting. The mice were anaesthetized using 75 mg/kg ketamine and 10 mg/kg xylazine, administered intraperitoneally. Lentiviral vectors were stereotaxically injected into the striatum using a 34-gauge blunt-tip needle linked to a Hamilton syringe (Hamilton, Reno, NV, USA) by a polyethylene catheter at the following stereotaxic coordinates: 0.5 mm rostral to bregma, 2 mm lateral to midline and 3.5 mm from the skull surface. Each mouse received the vector encoding mHtt alone in the left striatum and the vectors encoding both mHtt and AMPKγGOF in the right striatum.
Two hundred nanograms of p24 antigen of each viral vector was injected at 0.2 µl/min by means of an automatic injector (Stoelting Co., Wood Dale, USA) and the needle was left in place for an additional 5 min. The skin was closed using 4-0 Prolene suture (Ethicon, Johnson and Johnson, Brussels, Belgium).
3
Mechanical Characterization of Hydrogel-based Scaffolds
4
Establishing MI Mouse Model Procedure
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To establish an MI mouse model, 12-week-old male C57BL/6 mice (23 ± 4 g; KOATECH, Pyeongtaek, Republic of Korea) were used. Following anesthesia via zoletil (30 mg/kg; Virbac, France) and xylazine (10 mg/kg; Bayer Korea, Ansan, Republic of Korea), the mice were airway ventilated using a ventilator (Harvard Instruments, Holliston, MA, USA). The left anterior descending (LAD) artery was ligated with 6-0 prolene suture (Ethicon, Diegem, Belgium). Subsequently, muscle and skin closure were performed with 4-0 prolene suture (Ethicon) [21 ]. The mice were divided into three groups: sham, MI-1day, and MI-3day, based on the duration of left anterior descending LAD artery ligation, followed by suture closure [22 (link)]. The mice were sacrificed on the designated day to procure heart tissue for analysis, ensuring simultaneous tissue collection across all groups. Ten mice were selected for each group to establish an MI animal model, and finally, five mice were randomly selected per group for further experiments.
5
Coronary Artery Bypass Graft Procedure
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Conventional median sternotomy and preparation of conduits were undertaken by using a standard technique. In all the cases, the left internal mammary artery was anastomosed to the left anterior descending artery. For additional grafts, the radial artery and the greater saphenous vein were used. Cardiac exposure and stabilization was achieved by Octopus Tissue Stabilizer (Medtronic Inc., Minneapolis, MN, USA). The target vessels were exposed and snared above the anastomotic site by using a 4.0 prolene suture (Ethicon Inc., Somerville, NJ, USA) with a pledget to prevent coronary injury and reduce blood loss. An intracoronary shunt (Anastoflo Intravascular Shunt; Research Medical Inc., Salt Lake City, UT, USA) was used only in cases of hemodynamic instability. A cell-saving device was used routinely with frequent infusions of the salvaged blood as collected.
6
Suture Retention Strength of Vascular Scaffolds
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Suture retention testing of bilayered scaffolds and native rat aortas was performed according to ISO 7198 ''Cardiovascular implants-Tubular vascular prostheses,'' using a tensile tester (CellScale Biomaterials Testing). In brief, samples were cut into 10-mm long segments, gripped at one extremity by a clamp, and pierced through at the other extremity by a 4-0 prolene suture (Ethicon) at a distance of 2 mm from the sample's free edge, in accordance with the standard. For the suture retention tests 4-0 prolene was chosen because previous experience taught us that 8-0 ethilon sutures (which were used for the implantations) break before the scaffold breaks. Nine samples were tested in total [n = 5 of sterilized PC(e)-BU tubes and n = 4 of native rat aortas]. Tests were performed in saline at 37°C. Samples were stretched at 50 mm/min until rupture. Maximum force recorded before pull-through of the suture was considered to be the suture retention strength.
7
Tensile Strength of Bacterial Cellulose Grafts
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Wet BC grafts with 6 cm length (4 mm internal diameter and 7 mm external diameter) were fixed the top clamp of a Z2.5 Zwick/Roell (Zwick GmbH & Co. KG; Ulm; Germany). A 4-0 Prolene suture (0.15 mm diameter; Ethicon) was placed approximately 7 mm from the edge of the prosthesis and 2-3 cm from the top clamp (measured with a digital micrometer) and looped around the bottom clamp before clamping. The assay was then carried out in quintuplicate, on both freeze-dried and non-freeze-dried bacterial cellulose prosthetics, at a set speed of 10 mm Á min À1 and a 1 N pre-load.
8
Lentiviral Vector Injection in Striatum
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Concentrated viral stocks were thawed on ice and resuspended by repeated pipetting. The mice were anesthetized using 75 mg/kg ketamine and 10 mg/kg xylazine, administered intraperitoneally. LVs were stereotaxically injected into the striatum using a 34-gauge blunt-tip needle linked to a Hamilton syringe (Hamilton) by a polyethylene catheter at the following stereotaxic coordinates: 0.5 mm rostral to bregma, 2 mm lateral to midline, and 3.5 mm from the skull surface. Each mouse received vectors encoding HTT and a control siRNA LV targeting luciferase mRNA in the left striatum and vectors encoding both HTT and siRNA targeting HTT in the right striatum.
Each viral vector (200 ng of p24 antigen) was injected at a rate of 0.2 μL/min using an automatic injector (Stoelting) and the needle was left in place for an additional 5 min. The skin was closed using 4-0 Prolene sutures (Ethicon, Johnson and Johnson) for mice.
Each viral vector (200 ng of p24 antigen) was injected at a rate of 0.2 μL/min using an automatic injector (Stoelting) and the needle was left in place for an additional 5 min. The skin was closed using 4-0 Prolene sutures (Ethicon, Johnson and Johnson) for mice.
9
Sciatic Nerve Autograft Transplantation
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The right sciatic nerve was exposed through the transgluteal approach with sterile manner. In the donor mice, 10 mm of the sciatic nerve from the right hind limb was harvested using a 10-mm jig and microscissors and kept in cold phosphate-buffered saline (PBS). For recipient mice, a nerve gap was created by dissecting 3 mm of the sciatic nerve using a 3-mm jig and microscissors. As both resected ends retracted owing to the elastic recoil of the perineural tissue and the harvested nerve inevitably shrunk in length, the autograft harvested should be 25% longer than the recipient nerve gap to compensate for these changes 1, 13 (link) Half (5 mm) the dissected nerve section from donor mice was placed in the nerve gap of recipient mice, either in the normal or reversed proximodistal orientation. The autograft was kept well aligned and coapted with brin sealant (GC Pharma, Seoul, South Korea) at both resected ends under an operating microscope. The skin incision was closed using 4 or 5 interrupted 4-0 Prolene sutures (Ethicon, Somerville, NJ). Mice were then returned to their cages, allowed free activity, and were followed up under supervision of the attending veterinarian.
10
Inverted LDLLT Operative Technique
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The details of the operative technique of inverted LDLLT were previously reported [6, 7] . Briefly, after left pneumonectomy, the inverted right lower lobe graft was placed in the recipient left cavity. The bronchial anastomosis was performed first with 4-0 Prolene sutures (Ethicon, Tokyo, Japan). After the bronchial anastomosis, the pulmonary artery anastomosis was performed behind the bronchus with 6-0 Prolene sutures. This was possible because the recipient left main pulmonary artery remained longer than when noninverted LDLLT was performed. Finally, anastomosis of the pulmonary vein was performed with 6-0 Prolene sutures.
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