Total protein from brain tissue or BV2 cells was collected, and protein concentrations were determined with a BCA kit (Beyotime Institute of Biotechnology, China) following the manufacturer’s guidelines. Equal amounts of protein were separated by SDS-polyacrylamide gel electrophoresis, transferred to polyvinylidene difluoride membranes (Millipore, MA, USA), blocked with 5% skim milk, and incubated with primary antibodies (see below) either overnight at 4 °C or for 1 h at room temperature. The membranes were washed and incubated with a horseradish peroxidase-conjugated anti-rabbit IgG secondary antibody (1:500; Beyotime Institute of Biotechnology, China) and were visualized using an ECL Plus kit (Millipore). Densitometry was performed to quantify the signal intensity using ImageJ software (Version 1.45 J; National Institutes of Health, Bethesda, MD, USA). The primary antibodies were rabbit anti-Nrf2, anti-HO-1, anti-NF-κB1, anti-CD29, anti-CD90, anti-CD44, anti-CD105, anti-CD34, anti-vWF, anti-BDNF, anti-TrkB, and anti-GAPDH (Abcam, Cambridge, UK).
Anti nf κb1
Manufactured by Abcam
Sourced in Japan
Anti-NF-κB1 is a laboratory research tool that binds to the NF-κB1 protein. NF-κB1 is a transcription factor that regulates gene expression involved in immune response, inflammation, and cell survival. This product can be used to study the NF-κB1 protein and its role in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti bcl 2, by Abcam (2 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Ecl plus kit, by Merck Group (1 mentions)
Anti cd90, by Abcam (1 mentions)
Anti ho 1, by Abcam (1 mentions)
Horseradish peroxidase conjugated anti rabbit igg secondary antibody, by Beyotime (1 mentions)
Anti cd29, by Abcam (1 mentions)
Anti vwf, by Abcam (1 mentions)
Anti cd34, by Abcam (1 mentions)
Anti trkb, by Abcam (1 mentions)
Anti cd44, by Abcam (1 mentions)
Anti cd105, by Abcam (1 mentions)
Anti bdnf, by Abcam (1 mentions)
Bca kit, by Beyotime (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Anti mmp2, by Santa Cruz Biotechnology (1 mentions)
Anti mmp9, by Abcam (1 mentions)
Hrp conjugated anti rabbit secondary antibodies, by ProSci (1 mentions)
3 protocols using anti nf κb1
1
Western Blot Characterization of Cells
2
Protein Expression Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The primary antibodies used were anti-NF-κB1 (Abcam, Tokyo, Japan), anti-BCL2 (Abcam), anti-MMP2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti-MMP9 (Abcam). Protein samples were separated with 12% SDS-PAGE and transferred onto nitrocellulose membranes. Membranes were incubated with primary antibodies overnight at 4°C. Membranes were washed and incubated for 2 h with HRP-conjugated anti-rabbit secondary antibodies (ProSci, Poway, CA, USA), followed by detection and visualization using ECL Western blotting detection reagents (Pierce antibodies; Thermo Fisher Scientific).
3
Protein Expression Analysis by Western Blot
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After 72 h of transfection, the total protein of the abovementioned experimental group was extracted, quantified by Bradford method, and 20 μL was added to each sample (protein amount 30 μg). Protein samples were separated with 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes. Membranes were incubated with primary antibodies overnight at 4°C. Membranes were washed and incubated for 2 h with horseradish peroxidase-conjugated antirabbit secondary antibodies (ProSci, Poway, CA, USA), followed by detection and visualization using electrochemiluminescence Western blotting detection reagents (Pierce antibodies; Thermo Fisher Scientific). The primary antibodies used were anti-NF-κB1 (Abcam, Tokyo, Japan) and anti-Bcl-2 (Abcam).
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