The following antibodies were purchased from eBioscience (San Diego, CA): anti-CD8a-FITC, anti-CD8a-PE, anti-CD4-FITC, anti-CD4-PE, anti-IFN-γ-PE, anti-IFN-γ-FITC, anti-TNF-α-APC, anti-PD-1 (CD279)-FITC, and anti-rat IgG-FITC. The anti-CD8a-APC-Cy7 and anti-PD-1 (CD279)-PE antibodies were purchased from BioLegend (San Diego, CA) and the anti-CD127-Alexa fluor 647 antibody was from Bio-Rad Laboratories (Hercules, CA). The annexin V-FITC apoptosis detection kit was purchased from BioVision, Inc. (Milpitas, CA).
Anti cd8a fitc
Manufactured by Thermo Fisher Scientific
Anti-CD8a-FITC is a monoclonal antibody conjugated with the fluorescent dye FITC. It binds to the CD8a surface antigen, which is expressed on a subset of T cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd4 fitc, by Thermo Fisher Scientific (2 mentions)
Anti cd4 pe, by Thermo Fisher Scientific (1 mentions)
Anti ifn γ pe, by Thermo Fisher Scientific (1 mentions)
Anti ifn γ fitc, by Thermo Fisher Scientific (1 mentions)
Anti tnf α apc, by Thermo Fisher Scientific (1 mentions)
Anti rat igg fitc, by Thermo Fisher Scientific (1 mentions)
Anti cd8a apc cy7, by BioLegend (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Abcam (1 mentions)
Anti cd8a pe, by Thermo Fisher Scientific (1 mentions)
Facscalibur, by BD (1 mentions)
Anti cd25 pe, by Thermo Fisher Scientific (1 mentions)
Anti cd3 pe, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti cd8a fitc
1
Multicolor Flow Cytometry Panel
2
Isolation and Analysis of Murine Mesenteric Lymphocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mesenteric lymph nodes were isolated from each animal and cut into smaller pieces of approximately 1 mm2. After washing with phosphate buffer saline, the tissue was disrupted mechanically into smaller pieces. The remaining tissue aggregates were removed by a 70 μm nylon cell strainer. The resulting cell suspension was centrifuged twice at 1,000 rpm, for 5 min at 4°C in a 30% isotonic Percoll solution (Borunlaite Sci & Tech Co., Ltd., Beijing, China). The supernatant containing epithelial cells and debris was discarded; the cell pellet was washed and resuspended in RPMI1640 medium. Samples were stained with anti-CD3 PE (12-0030-82, eBioscience, San Diego, Calif), anti-CD4 FITC (11-0040-82, eBioscience, San Diego, Calif), anti-CD8a FITC (11-0084-82, eBioscience, San Diego, Calif), anti-CD25 PE (12-0390-82, eBioscience, San Diego, Calif), anti-IL17RB (BS-2610R, Bioss, Beijing, China), and anti-Foxp3 eFluor 660 (50-5773-82, eBioscience, San Diego, Calif). Flow cytometry data were acquired using a FACSCalibur (BD Biosciences, San Diego, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR).
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