Sureprint g3 mouse ge microarray kit

Total RNA from embryos was hybridized using a SurePrint G3 mouse GE microarray kit 8 × 60 K v3 (Agilent). The microarray data are available on the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) (accession no. GSE146614). Gene ontology (GO) analysis of the differentially expressed genes with a Z-score of over 2 or less than −2 was performed using The Database for Annotation, Visualization and Integrated Discovery (DAVID) Bioinformatics Resources 6.8. Hierarchical clustering analysis and heatmap drawing were performed using the “pheatmap” package in The Comprehensive R Archive Network with R (version 3.6.1).

Total RNA was isolated from BAT, gWAT and iWAT of Adipo-Pgam1 KO with RNeasy Lipid Tissue Kit (QIAGEN). Cyanine-3 (Cy3) labeled cRNA was prepared from 600 ng RNA using the One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling) (Agilent, CA, USA) according to the manufacturer’s instructions, and the resulting probes were hybridized to Agilent SurePrint G3 Mouse GE Microarray kit (8 × 60K) (G4852B). The scanned images were normalized by Agilent GeneSpring GX 12.0 software and differentially expressed genes were identified via the fold-change (FC) and p values of the t-test. Gene expression data are available through the Gene Expression Omnibus database (GSE186030).