Sso fast evagreen real time pcr

Manufactured by Bio-Rad

The SsoFast EvaGreen Real-Time PCR is a reagent kit designed for real-time quantitative PCR (qPCR) analysis. It contains a premixed solution that includes the DNA polymerase, buffer, and EvaGreen dye, which binds to double-stranded DNA and emits a fluorescent signal during amplification.

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Lab products found in correlation

Cfx96 thermocycler, by Bio-Rad (3 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Rneasy mini kit, by Qiagen (1 mentions) Mmlv reverse transcriptase, by Bio-Rad (1 mentions) Superscript 3 cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Sybr green probe, by Bio-Rad (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions)

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EvaGreen (51 citations)

3 protocols using sso fast evagreen real time pcr

Cardiac Tissue RNA Isolation and RT-PCR

Cited 1 time

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Samples from Wild Type, nesprin 1^f/f;nesprin 2^+/+;Nkx2.5Cre, nesprin 1^f/f;nesprin 2^−/− and nesprin 1^f/f;nesprin 2^−/−;Nkx2.5Cre hearts, isolated cardiomyocytes or stretch samples were homogenized in Trizol reagent (Invitrogen) or RNeasy Mini Kit (QIAGEN)to isolate total RNA. First strand cDNA was generated utilizing random hexamers and the Invitrogen Superscript II kit (Invitrogen). Primers for RT-PCR were used as previously described [31] (link) or as listed in Supplementary Table 1. These primers were optimized using control murine cDNA (data not shown). RT-PCR reactions were performed using Sso-Fast EvaGreen Real Time PCR (BioRad) master mix in 96 well low profile PCR plates in the CFX96 BioRad thermocycler (BioRad).

Banerjee I., Zhang J., Moore-Morris T., Pfeiffer E., Buchholz K.S., Liu A., Ouyang K., Stroud M.J., Gerace L., Evans S.M., McCulloch A, & Chen J. (2014). Targeted Ablation of Nesprin 1 and Nesprin 2 from Murine Myocardium Results in Cardiomyopathy, Altered Nuclear Morphology and Inhibition of the Biomechanical Gene Response. PLoS Genetics, 10(2), e1004114.

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Cardiac Gene Expression Profiling

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Total RNA was extracted from whole heart tissue using Trizol reagent (Life Technologies) according to the manufacturer’s instructions. cDNA was synthesized using MMLV Reverse Transcriptase (Bio-Rad). Primer sequences for qRT-PCR are listed in Supplementary Table 1. RT-PCR reactions were performed using Sso-Fast EvaGreen Real Time PCR (Bio-Rad) master mix in 96-well low profile PCR plates in the CFX96 Biorad Thermocycler.

Zhang Z., Mu Y., Veevers J., Peter A.K., Manso A.M., Bradford W.H., Dalton N.D., Peterson K.L., Knowlton K.U., Ross R.S., Zhou X, & Chen J. (2016). Postnatal Loss of Kindlin-2 Leads to Progressive Heart Failure. Circulation. Heart failure, 9(8), 10.1161/CIRCHEARTFAILURE.116.003129 e003129.

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Quantitative Expression Analysis of Embryonic Heart

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Total RNA was isolated from homogenized embryonic hearts using TRIzol (Invitrogen) and reverse transcribed to cDNA using a Super Script III cDNA Synthesis Kit (Invitrogen), according to the manufacturer’s instructions. Complementary DNA amplicons were quantified by incorporation of SYBR Green probe (Biorad) into dsDNA. RT-PCR reactions were performed using Sso-Fast EvaGreen Real Time PCR (Bio-Rad) master mix in 96-well low profile PCR plates in the CFX96 Biorad Thermocycler. Samples were compared using the relative (comparative) Ct method after adjusting for 18s RNA (ΔCt). Primers used in this study are listed in S2 Table.

Mu Y., Yu H., Wu T., Zhang J., Evans S.M, & Chen J. (2020). O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1. PLoS Genetics, 16(4), e1008730.

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