Hplc grade methanol

Sodium acetate, FeCl₃·6H₂O, polyethylene glycol 6000 (PEG 6000), cetyltrimethyl ammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), acetonitrile, ethanol, acetic acid, acetone and HPLC grade methanol were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Acrylamide (AM), acrylic acid (AA), methylacrylic acid (MAA), 4-vinylpyridine (4-VP), ethylene glycol dimethacrylate (EGDMA), and 3-(trimethoxysily) propyl methacrylate (MPS) were acquired from Shanghai Macklin Biochemical Co, Ltd. (Shanghai, China). 2,2′-azobis-(isobutyronitrile) (AIBN) was provided by Chinasun Specialty Products co, Ltd. (Jiangsu, China). Cartap with purities over 98% was purchased from RAM.M Reagent Company, China. Nereistoxin and bensultap with purities over 99% were obtained from Alfa Chemistry, New York, NY, USA. Both bisultap and monosultap with purities over 99% were from Crescent Chemical Company, Islandia, NY, USA. Triple distilled water (deionized water) newly collected from a glass distillator (Yarong, Shanghai, China) was used to prepare solutions. Other reagents were of analytical grade and obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Six tea samples (green, black, white, yellow, dark and oolong teas), bottled green tea beverage, and bottled iced black tea beverage were purchased from a local supermarket.

Dulbecco’s modified essential medium (DMEM, high glucose) and fetal bovine serum (FBS) were purchased from Gibco Industries Inc. (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO) was purchased from Sigma—Aldrich (St. Louis, MO, USA). An Annexin V FITC apoptosis detection kit was obtained from Dojindo Molecular Technologies (Kumamoto, Japan). Trypsin (0.25% with or without EDTA), CCK-8, cell cycle detection kit and reactive oxygen species (ROS), Mitochondrial Membrane Potential Detection Kit (C1071; Beyotime, Shanghai, China) assay kit were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). The fruiting bodies of P. baumii were obtained from Huqingyutang drugstore (Hangzhou, China).
HPLC-grade methanol was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Protocatechuic aldehyde, caffeic acid, osmundacetone and hispidin (all HPLC grade) were obtained from Weikeqi Biological Technology Co., Ltd. (Chengdu, China).

SMX (analytical standard, > 99.5%), HPLC-grade methanol, and acetonitrile were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd. All other reagents used in the present study were of analytical grade. Mineral salt medium (MSM) and Luria–Bertani (LB) medium were used as described by Song et al^{25 (link)}. Agar plates were prepared by adding 1.5% (w/v) agar into the liquid media.

Extraction of atrazine from soil samples was performed in the manner described by Krutz et al. [17 (link)]. The extracts were analyzed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) on an UltiMate 3000 HPLC system interfaced to a TSQ Vantage Triple Quadrupole Mass Spectrometer (Thermo Fisher Scientific, USA). The conditions of HPLC and MS/MS parameters are provided in Additional file 12. Calibration standards of atrazine (# 45330 Sigma-Aldrich, USA) were prepared in HPLC grade methanol (Sinopharm, China) at the following concentrations: 0.01, 0.05, 0.1, 0.5, 1, 5, 10, 50 and 100 ng mL⁻¹. The typical calibration curve was Y = 2313.0 + 4073.8X. The curve displayed excellent linearity (r² = 0.9995) over the entire calibration range. Recovery of atrazine from fortified soil samples was 90.3–93.2 %. The method limit of atrazine quantification was 0.05 μg kg⁻¹.