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35s protein labeling mix

Manufactured by PerkinElmer
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The [35S] Protein Labeling Mix is a radioactive labeling reagent used for the detection and quantification of newly synthesized proteins in cell-based assays. It contains [35S]-L-methionine that can be incorporated into proteins during translation, enabling the visualization and analysis of protein expression patterns.

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Lab products found in correlation

Protein a sepharose, by Cytiva (1 mentions) Forskolin, by Bio-Techne (1 mentions) Zardaverine, by Bio-Techne (1 mentions) Isoproterenol, by Merck Group (1 mentions) Kt5720, by Bio-Techne (1 mentions) Ici 118 551, by Merck Group (1 mentions) Nkh 477, by Bio-Techne (1 mentions) Ro 20 1724, by Bio-Techne (1 mentions) Cgp20712a, by Merck Group (1 mentions) R rolipram, by Bio-Techne (1 mentions) Epinephrine, by Merck Group (1 mentions) 3h thymidine, by PerkinElmer (1 mentions) Gel drier, by Bio-Rad (1 mentions) Typhoon imager, by GE Healthcare (1 mentions)

Close spelling variants of this product

Easy Tag 35S Protein Labeling Mix (3 citations) [35S] EXPRESS35S Protein Labeling Mix (3 citations) [35S]methionine-cysteine ([35S] protein labeling mix; (3 citations) 35S-methionine/cysteine protein labeling mix (4 citations) EASY-TAG EXPRESS Protein labeling mix [35S] (3 citations) Express [35S]Protein Labeling Mix (1.175 Ci/mmol) (2 citations) [35S]Met and [35S]Cys protein labeling mix (2 citations) EasyTag EXPRESS35S Protein Labeling Mix [35S] (2 citations) EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (2 citations) EXPRESS 35S Protein Labeling Mix (23 citations)

5 protocols using 35s protein labeling mix

1

Examining Env Glycoprotein Processing

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293T cells (3 × 105) were transfected by the calcium phosphate method with the different IMCs. One day after transfection, cells were metabolically labeled for 16 h with 100 μCi/mL of [35S]methionine-cysteine ([35S] Protein Labeling Mix; PerkinElmer, Waltham, MA, USA) in DMEM lacking methionine and cysteine and supplemented with 5% dialyzed fetal bovine serum. Cells were subsequently lysed in RIPA buffer (140 mM NaCl, 8 mM Na2HPO4, 2 mM NaH2PO4, 1% NP40, 0.05% sodium dodecyl sulfate (SDS), 1.2 mM sodium deoxycholate). Precipitation of radiolabeled envelope glycoproteins from the whole-cell lysates or found in the supernatant was performed with a pool of sera from HIV-1-infected individuals in the presence of 50 μL of 10% Protein A-Sepharose (Cytiva, Marlborough, MA) at 4 °C. The precipitated proteins were loaded onto SDS-PAGE gels and analyzed by autoradiography and densitometry to calculate their processing indexes. The processing index is a measure of the conversion of the mutant gp160 Env precursor to mature gp120, relative to wild-type Env trimers. The processing index is calculated with the following formula: processing index = ([total gp120]mutant × [gp160]WT)/([gp160]mutant × [total gp120]WT).
Prévost J., Medjahed H., Vézina D., Chen H.C., Hahn B.H., Smith AB I.I.I, & Finzi A. (2021). HIV-1 Envelope Glycoproteins Proteolytic Cleavage Protects Infected Cells from ADCC Mediated by Plasma from Infected Individuals. Viruses, 13(11), 2236.
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2

Synthesis of MNF Stereoisomers and Fenoterol

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(R,R’), (S,R ’), (R,S’) and (S,S’) stereoisomers of MNF as well as (R,R’)-Fenoterol (Fen) were synthesized according to previously reported synthesis scheme [1 (link), 18 (link)]. Forskolin, NKH-477, Ro 20–1724, (R)-(−)-rolipram, zardaverine, H-89 and KT 5720 were purchased from Tocris Bioscience (Ellisville, MO, USA). Epinephrine, isoproterenol, ICI-118,551 and CGP-20712A were obtained from Sigma-Aldrich (St. Louis, MO, USA). Tritiated compounds, [3H]CGP-12177 (41.6 Ci/mmol), [3H]thymidine (10 Ci/mmol) and [35S]-protein labeling mix (0.1 Ci/mmol) were from PerkinElmer Life and Analytical Sciences (Waltham, MA, USA).
Wnorowski A., Sadowska M., Paul R.K., Singh N.S., Boguszewska-Czubara A., Jimenez L., Abdelmohsen K., Toll L., Jozwiak K., Bernier M, & Wainer I.W. (2015). Activation of β2-adrenergic receptor by (R,R’)-4’-methoxy-1-naphthylfenoterol inhibits proliferation and motility of melanoma cells. Cellular signalling, 27(5), 997-1007.
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3

Radiolabeling Proteins in Huh7 Cells

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5×105 Huh7 cells were seeded in 6-wells. After infection as above, cells were washed once with PBS then incubated with 100 μCi/mL35S protein labeling mix (PerkinElmer) in 1.5 ml cysteine and methionine free DMEM (Thermo) for 1 h at 37°C. Cells were washed twice with PBS then lysed in RIPA buffer as described above. Samples were resolved on homemade 10% SDS-PAGE, which was dried for 2 h at 70°C using a gel drier (Bio-Rad). Autoradiography detection was performed using x-ray films.
Aviner R., Li K.H., Frydman J, & Andino R. (2021). Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis. Nature, 596(7873), 558-564.
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4

Metabolic Labeling of Proteins

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60–70% confluent cells from 6-well plates were used for the labelling. Cells were incubated with mESC medium supplemented with 0.8 Mbq/ml of 35S Protein Labeling Mix (Perkin Elmer) at 37°C with 5% CO2 for 30 min. Cells were lysed, lysates were loaded into SDS-PAGE gels as described in the previous section. Then the gel was fixed with 30% methanol and 7% acetic acid for 40 min. The fixed gel was dried using a vacuum gel dryer for 1 h. Then the dried gel was exposed to a phosphor plate before the scanning using Typhoon imager (GE).
Liang S., Silva J.C., Suska O., Lukoszek R., Almohammed R, & Cowling V.H. (2022). CMTR1 is recruited to transcription start sites and promotes ribosomal protein and histone gene expression in embryonic stem cells. Nucleic Acids Research, 50(5), 2905-2922.
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5

Measuring gp120 Env trimer association

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3 × 105 293T cells were transfected by the calcium phosphate method with the different IMCs. One day after transfection, cells were metabolically labeled for 16 h with 100 μCi/mL [35S]methionine-cysteine ([35S] Protein Labeling Mix; Perkin-Elmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 5% dialyzed fetal bovine serum. Cells were subsequently lysed in RIPA buffer (140 mM NaCl, 8 mM Na2HPO4, 2 mM NaH2PO4, 1% NP40, 0.05% sodium dodecyl sulfate (SDS)). Precipitation of radiolabeled envelope glycoproteins from cell lysates or medium was performed with a mixture of sera from HIV-1-infected individuals in the presence of 50 μl of 10% Protein A-Sepharose (American BioSciences) at 4 °C. The association index is a measure of the ability of the mutant gp120 molecule to remain associated with the Env trimer complex on the expressing cell, relative to that of the wild-type Env trimers. The association index is calculated as follows: association index = ([mutant gp120]cell × [wild-type gp120]supernatant)/([mutant gp120]supernatant × [wild-type gp120]cell).
Prévost J., Richard J., Ding S., Pacheco B., Charlebois R., Hahn B.H., Kaufmann D.E, & Finzi A. (2017). Envelope glycoproteins sampling states 2/3 are susceptible to ADCC by sera from HIV-1-infected individuals. Virology, 515, 38-45.
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