Murine C5a was from Cell Sciences (Canton, ME). Anti-mouse C3a and C5a mAbs were from BD Biosciences (San Jose, CA). C3ar1-A and C5ar1-A were purchased from Calbiochem (EMB Biochemicals). CFSE was used according to the manufacturer’s instructions (Invitrogen). Anti-mouse IgM F(ab’)2 was purchased from Jackson Labs (Bar Harbor, ME). FITC anti-mouse CD19, anti-mouse CD40, PE anti-mouse CD40, anti-mouse IL-6, biotin-anti-mouse IL-6, anti-mouse C5a, biotin anti-mouse C5a, BAFF, APRIL, PE anti-mouse TACI, and APC anti-mouse BAFF-R were purchased from BD Biosciences (San Jose, CA). HRP anti-mouse IgG was purchased from Cell Signal Technology (Danvers, MA) Recombinant mouse IL-4 was from Miltenyi Biotech, (San Diego, CA). and LPS (Escherichia coli O26:B6) from Sigma-Aldrich (St. Louis, MO)
Pe anti mouse cd40
Manufactured by BD
Sourced in United States
PE anti-mouse CD40 is a fluorescently-labeled monoclonal antibody that specifically binds to the mouse CD40 antigen. CD40 is a cell surface receptor expressed on various immune cells, including B cells, dendritic cells, and macrophages. This antibody can be used for the identification and analysis of CD40-positive cells in flow cytometry applications.
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Lab products found in correlation
Anti mouse igg hrp, by Cell Signaling Technology (1 mentions)
Recombinant mouse il 4, by Miltenyi Biotec (1 mentions)
Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions)
Anti mouse cd40, by BD (1 mentions)
Fitc anti mouse cd19, by BD (1 mentions)
Lps escherichia coli o26 b6, by Merck Group (1 mentions)
Anti mouse igm f ab 2, by Jackson ImmunoResearch (1 mentions)
April, by BD (1 mentions)
Fitc antimouse cd86 gl1, by BD (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
2 protocols using pe anti mouse cd40
1
Murine C5a: A Comprehensive Immunological Assay
2
Multiparameter Flow Cytometry Analysis
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All flow cytometric analysis was performed using an LSR II flow cytometer (BD Biosciences) and analyzed with FlowJo version 10.0.7 (Tree Star Inc., Ashland, OR, USA). Antibodies used for flow cytometry: eFluor® 450 antimouse CD3ε (145-2C11, eBioscience, San Diego, CA, USA), PE-Cy7 antimouse NK1.1 (PK136, BD Pharmingen), PE-Cy7 antimouse CD19 (1-D3, BD Pharmingen), PE antimouse CD4 (GK1.5, TONBO Biosciences, San Diego, CA, USA), FITC antimouse CD8α (53-6.7, BD Pharmingen), APC antimouse IFN-γ (XMG1.2, TONBO Biosciences), Pacific Blue antimouse CD11c (N418, Biolegend, San Diego, CA, USA), FITC antimouse CD86 (GL1, BD Pharmingen), PE antimouse CD40 (3/23, BD Pharmingen), PE antimouse I-Ab (AF6-120.1, BD Pharmingen), PE-Cy5 antimouse CD11b (M1/70, TONBO Biosciences), PE-Cy7 antimouse CD8α (53-6.7, TONBO Biosciences), and APC-Cy7 antimouse CD103 (2E7, Biolegend).
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