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Anti β catenin 9582

Manufactured by Cell Signaling Technology
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Anti-β-catenin (9582) is a primary antibody product offered by Cell Signaling Technology. The antibody is specific for the detection of β-catenin, a key regulator of the Wnt signaling pathway. This product is intended for use in various experimental techniques, such as western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of β-catenin in biological samples.

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Lab products found in correlation

Anti odam 16509 1 ap, by Proteintech (2 mentions) Anti β actin a5441, by Merck Group (1 mentions) Horseradish peroxidase conjugated goat anti mouse or anti rabbit igg, by GE Healthcare (1 mentions) Ecl detection system, by GE Healthcare (1 mentions) Dihydroethidium dhe, by Merck Group (1 mentions) N acetly l cysteine nac, by Merck Group (1 mentions) Anti β actin lk9001t, by Sungene Biotech (1 mentions) Anti gapdh lk9002t, by Sungene Biotech (1 mentions) Dako envision dual link system hrp, by Agilent Technologies (1 mentions)

Spelling variants of this product

β-catenin (1138 citations) Anti-β-catenin (433 citations) β-catenin (9582) (2 citations)

3 protocols using anti β catenin 9582

1

Western Blot Analysis of ODAM, β-catenin, and β-actin

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Anti‐ODAM (16509‐1‐AP, Proteintech, Chicago, IL), anti‐β‐catenin (9582, Cell Signaling Technology, Danvers, MA), and anti‐β‐actin (A5441, Sigma‐Aldrich) antibodies were used for western blot analysis. Horseradish peroxidase‐conjugated goat anti‐mouse or anti‐rabbit IgG (GE Healthcare) served as the secondary antibody for the ECL Detection System (GE Healthcare).
Yamaguchi K., Horie C., Takane K., Ikenoue T., Nakagawa S., Isobe Y., Ota Y., Ushiku T., Tanaka M., Fujishiro J., Hoshino N., Arisue A., Nishizuka S., Aikou S., Shida D, & Furukawa Y. (2022). Identification of odontogenic ameloblast associated as a novel target gene of the Wnt/β‐catenin signaling pathway. Cancer Science, 114(3), 948-960.
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2

Ovarian Cancer Cell Line Culture

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Human ovarian cancer cell lines A2780, OVCAR3, SKOV3, HO8910, HO8910PM, ES-2 and normal embryonic kidney cell line HEK293T were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FBS), penicillin (100 U/ml) and streptomycin (100 ng/ml) in a humidified incubator at 37°C with 5% CO2. Dinaciclib and cisplatin were ordered from ApeBio and Qilu Pharmaceutical, respectively. N-acetly-L-cysteine (NAC) and dihydroethidium (DHE) were purchased from Sigma-Aldrich. Anti-pRb pS807/S811 (9308), Anti-PARP (9542), Anti-Mcl-1 (4572), Anti-Survivin (2808), Anti-XIAP (2045) and Anti-β-Catenin (9582) antibodies were from Cell Signaling Technologies. Anti-CDK2 (SC-163), Anti-CDK5 (SC-173), Anti-Cyclin A (SC-596), Anti-Cyclin E (SC-481), Anti-HSP90 (SC-13119), Anti-MDM2 (SC-965), and Anti-Raf-1 (SC-133) antibodies were from Santa Cruz Biotechnology. Anti-p27 (610241), Anti-Cyclin B (610219) and Anti-CDK1 (610037) antibodies were from BD Bioscences. Anti-CDK9 (2496-1) was from Epitomics. Anti-β-Actin (LK9001T) and Anti-GAPDH (LK9002T) antibodies were from Tianjin Sungene Biotech.
Chen X.X., Xie F.F., Zhu X.J., Lin F., Pan S.S., Gong L.H., Qiu J.G., Zhang W.J., Jiang Q.W., Mei X.L., Xue Y.Q., Qin W.M., Shi Z, & Yan X.J. (2015). Cyclin-dependent kinase inhibitor dinaciclib potently synergizes with cisplatin in preclinical models of ovarian cancer. Oncotarget, 6(17), 14926-14939.
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3

Immunohistochemical Detection of ODAM and β-Catenin

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Immunohistochemical staining was performed using anti‐ODAM (16509‐1‐AP, Proteintech) and anti‐β‐catenin (9582, Cell Signaling Technology) antibodies as described previously.16 Dako EnVision+ Dual Link System‐HRP (Dako, Carpentaria, CA), and Dako REAL EnVision Detection System, Peroxidase/DAB+, Rabbit/Mouse (Dako) were used to visualize the immunoreactivity.
Yamaguchi K., Horie C., Takane K., Ikenoue T., Nakagawa S., Isobe Y., Ota Y., Ushiku T., Tanaka M., Fujishiro J., Hoshino N., Arisue A., Nishizuka S., Aikou S., Shida D, & Furukawa Y. (2022). Identification of odontogenic ameloblast associated as a novel target gene of the Wnt/β‐catenin signaling pathway. Cancer Science, 114(3), 948-960.
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