Glucoamylase

The broken rice used to produce the hydrolysate in this study was the by-product of rice processing provided by Zhongkou Fu Rice Industry Co Ltd, Dongguan, China. The microalgae strain used in this study, C. vulgaris FACHB-32, was obtained from the Institute of Hydrobiology, Chinese Academy of Science, P. R. China. The soybeans used in this study were purchased from the market in Nanchang, China.
The preparation of hydrolysate from broken rice was based on the method former reported [18 (link)]. Briefly, broken rice was gelatinized in boiling water for 30 min, and then hydrolyzed with 0.24% α-amylase (3,700 U/g, Solarbio Science and Technology Co., Ltd.) and 0.59% glucoamylase (10⁵ U/g, Solarbio Science and Technology Co., Ltd.). After that, the sample was boiled to inactivate the enzyme, and filtered with 0.45 µm filter membrane and stored at 4 °C for later use. The concentration of TOC, reducing sugar and TN content in BRH were 39.04, 87.12 and 0.81 g/L, respectively.

Ganoderma sinense (CGMCC: 5.69) was acquired from the Institute of microbiology, Chinese Academy of Sciences. The full-fat rice bran (FRB) and defatted rice bran (DRB) were purchased from Henan Siwei Biotech Co., ltd (Zhengzhou, China). a-Amylase (50 U/mg), gluco-amylase (70 U/mg) and trypsin (4000 U/g) were purchased from Solarbio (Beijing, China). Monosaccharide standards were purchased from Sigma-Aldrich (Shanghai, China). H1299 cell line was provided by ATCC (USA). Dulbecco’s Modified Eagle’s Medium (DMEM), serum free medium (SFM) and Fetal Bovine Serum (FBS) were provided by GIBCO Company (USA), BALB/c-nu nude mice (female, 20 ± 2 g) were provided by Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (Beijing, China). Phosphate buffered saline (PBS), Dimethyl sulfoxide (DMSO) and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltertrazolium bromide (MTT) were purchased from Solarbio (Beijing, China).