300mesh grids

Manufactured by Quantifoil

The 300mesh grids are a type of lab equipment used in electron microscopy. They provide a substrate for holding and supporting thin samples for analysis under the microscope. The 300mesh grids have a grid pattern with 300 squares per inch, which is a common specification for this type of product.

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Lab products found in correlation

Easiglow, by Ted Pella (1 mentions)

2 protocols using 300mesh grids

TEM Characterization of Pt Nanoparticles

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For TEM analysis, a Jeol 2100
microscope operated at 200 kV was used. For TEM, the Pt nanoparticles
were washed and redispersed in pure ethanol (99.9%, Kemetyl) and diluted
50 times as previously described.^{7 (link)} Small
drops of colloids then were placed on carbon-coated copper grids (300
mesh grids, Quantifoil) and dried in room conditions. The size and
size distribution analyzes were performed by measuring the size of
typically 200 (at least 165) nanoparticles with ImageJ software, and
samples were characterized by taking images of (at least) three different
magnifications in (at least) five different areas of the TEM grids.
The log–normal distribution parameters were obtained using
the fit function of OriginPro software on the relevant data set.

Quinson J., Kacenauskaite L., Christiansen T.L., Vosch T., Arenz M, & Jensen K.M. (2018). Spatially Localized Synthesis and Structural Characterization of Platinum Nanocrystals Obtained Using UV Light. ACS Omega, 3(8), 10351-10356.

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Cryo-EM Imaging of TbCSV Particles

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Cryo-EM grids were prepared by applying TbCSV particles to 300 mesh grids (Quantifoil Micro Tools GmbH). Grids were glow-discharged for 60 s before applying the sample (easiGlow, Ted Pella). 2.5 μL sample was applied to the grids, blotted immediately using a FEI Vitrobot Mark IV plunge freeze (Thermo Fisher Co) device. Grids were frozen in liquid ethane cooled by liquid nitrogen at 100% relative humidity, and a chamber temperature of 20 °C. TbCSV data were collected on an FEI Titan Krios G2 (Center of Cryo Electron Microscopy, Zhejiang University) EM at 300 kV, using a total electron dose of 40 e-/Å² and a magnification of 29,000×. A total of 2495 movies were recorded using the SerialEM [16 (link)] semi-automated acquisition software on a Gatan K2 summit direct electron detector in counting mode, with a final pixel size of 1.014 Å/pixel. Each exposure movie had a total exposure of 5 s and contained 20 frames.

Xu X., Zhang Q., Hong J., Li Z., Zhang X, & Zhou X. (2019). Cryo-EM Structure of a Begomovirus Geminate Particle. International Journal of Molecular Sciences, 20(7), 1738.

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