Rituximab was procured from F. Hoffmann-La Roche, Ltd (100 mg, Basel, Switzerland). Indocyanine green for injection was purchased from Dandong Yichuang Pharma, Ltd (25 mg, Dandong, Liaoning, PR China). Cyclone storage phosphor system was provided by the Department of Nuclear Medicine, Shandong University (Jinan, PR China). Dialysis tube was purchased from Spectrum Laboratories, Inc (Flat Width 24 mm, Molecular Weight Cut Off 3.5–5.0 kD, Rancho Dominguez, USA). Electrophoresis apparatus was procured from Bio-Rad Laboratories, Inc (Richmond, CA, USA). Silica gel plate for thin layer chromatography, enzyme linked immune sorbent assay (ELLAS) kit, and cell culture mediums were obtained from Sigma-Aldrich (Shanghai, PR China). All chemicals and reagents were of analytical grade.
Cell culture medium
Manufactured by Merck Group
Sourced in United States, Germany, Sweden, United Kingdom, Italy
Cell culture medium is a liquid or semi-solid nutrient solution designed to support the growth and maintenance of living cells in a controlled laboratory environment. It provides essential components such as amino acids, vitamins, salts, and other nutrients required for cell survival and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Trypan blue, by Merck Group (4 mentions)
Sulforodamine b, by Merck Group (3 mentions)
Alamar blue, by Merck Group (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Formic acid, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Diaspase, by Thermo Fisher Scientific (2 mentions)
Gum acacia, by Santa Cruz Biotechnology (2 mentions)
Phosphate buffered saline (pbs), by GE Healthcare (2 mentions)
Rnaimax, by Thermo Fisher Scientific (2 mentions)
Taqman advanced mirna cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Electrophoresis apparatus, by Bio-Rad (1 mentions)
Rituximab, by Roche (1 mentions)
Reference compounds, by Merck Group (1 mentions)
Jzl195, by Cayman Chemical (1 mentions)
Genistein, by Extrasynthese (1 mentions)
Serum and antibiotics, by Thermo Fisher Scientific (1 mentions)
35 protocols using cell culture medium
1
Rituximab-based Imaging and Analysis
2
Comprehensive Analysis of Diverse Compounds
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Mass Spectroscopy (MS) grade methanol, acetonitrile (ACN), water and formic acid (FA) for UPLC–MS analyses were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). Estradiol valerate (Progynova® 2 mg) was purchased from DELPHARM (Lille, France). Genistein was obtained from “Extrasynthese®” (Genay, France). The penicilline (xtapen®) was provided by CSPC Zhongnuo pharmaceutical (Shijiazhuang City, China). The Diclofenac (Dicloecnu®) was provided by ECNU pharmaceutical (Yanzhou City, China). Serums and antibiotics were purchased from GIBCO (Grand Island, NY). The 17β-estradiol benzoate [(Estr-1,3,5(10)-trien-3,16α,17β-triol); purity ≥ 98%] was obtained from Sigma-Aldrich (Hamburg, Germany). The 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane sulfonic acid (HEPES, purity ≥ 99.5%) was purchased from Ludwig Biotecnologia Ltda (Alvorada, RS, Brazil). Trypan blue, Alamar blue, Sulforodamine B and cell culture mediums were purchased from Sigma-Aldrich (St. Louis, MO, USA). The Smart Button Data loggers were purchased from ACR System Inc (Surrey, Canada).
3
FAAH Inhibition Assay Protocol
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Reference compounds, cell culture mediums and other reagents, along with plates and other physical supports for cell culture and enzyme assays were purchased from Merck Sigma (Milan, Italy) and Invitrogen (Basel, Switzerland). The FAAH enzyme, the substrate AMC-AA and the reference compound JZL-195 for the FAAH inhibition assay were obtained from Cayman Chemical (Ann Arbor, MI, USA).
4
Evaluating Anti-Inflammatory and Estrogenic Effects
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Serum and antibiotics were purchased from GIBCO (Grand Island, NY). The 17β-estradiol benzoate (Estr-1,3,5(10)-trien-3,16α,17β-triol) was obtained from Sigma-Aldrich (Hamburg, Germany). The 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane sulfonic acid (HEPES) was purchased from Ludwig Biotecnologia Ltda (Alvorada, RS, Brazil). Trypan blue, Sulforodamine B, Alamar blue and cell culture mediums were purchased from Sigma-Aldrich (St. Louis, MO, USA). Genistein was obtained from “Extrasynthese®” (Genay, France). Penicillin (xtapen®) was provided by CSPC Zhongnuo pharmaceutical (Shijiazhuang City, China). Diclofenac (Dicloecnu®) was provided by ECNU pharmaceutical (Yanzhou City, China). The Smart Button Data loggers were purchased from ACR System Inc (Surrey, Canada).
5
Cytotoxicity Assay for Cell Lines
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Serums and antibiotics were purchased from GIBCO (Grand Island, NY, USA). The 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane sulfonic acid (HEPES, purity ≥ 99.5%) was purchased from Ludwig Biotecnologia Ltda. (Alvorada, RS, Brazil). DMBA (purity ≥ 95%) was purchased from Sigma-Aldrich (Stanford, Germany). Trypan blue (0.4%), Alamar Blue, and cell culture mediums were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tamoxifen citrate (Mylan®) was purchased from Mylan SAS (Saint-Priest, France). All solutions and buffers were prepared in Ultrapure Milli-Q water.
6
Estrogen Compound Characterization and Cell Viability Assay
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Mass Spectroscopy (MS) grade methanol, acetonitrile (ACN), water and formic acid (FA) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). Fetal bovine serum (FBS) and antibiotics were purchased from GIBCO (Grand Island, NY). The 17β-estradiol benzoate [(Estr-1,3,5(10)-trien-3,16α,17β-triol); purity ≥98%] was obtained from Sigma-Aldrich (Hamburg, Germany). Estradiol valerate (Progynova® 2 mg) was purchased from DELPHARM (Lille, France). The 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane sulfonic acid (HEPES, purity ≥99.5%) was purchased from Ludwig Biotecnologia Ltda (Alvorada, RS, Brazil). Trypan blue, Sulforodamine B, Serum Replacement 2 and cell culture mediums were purchased from Sigma-Aldrich (St. Louis, MO, USA). Genistein was obtained from “Extrasynthese®” (Genay, France).
7
Cryopreserved Hepatocytes in Cell Culture
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Cryopreserved PHH were purchased from BioreclamationIVT (Brussels, Belgium) and thawed using InVitroGRO CP medium or Cryopreserved Hepatocyte Recovery Medium (Thermo Fisher Scientific, USA). Cells were seeded in 96-well ultra-low attachment plates (Corning, USA) in culture medium (Williams’ medium E, supplemented with 2 mM L-glutamine, 100 U/ml penicillin, 100 mg/ml streptomycin, 10 mg/ml insulin, 5.5 mg/ml transferrin, 6.7 ng/ml sodium selenite, and 100 nM dexamethasone) with 10% fetal bovine serum (FBS) as previously described (Bell et al., 2016 (link)). Cryopreserved primary animal hepatocytes were obtained from BioreclamationIVT (Gottingen minipig, Wistar rat, rhesus monkey, CD1 mouse) or Lonza (USA; C57BL/6 mouse; hereafter called BL6) and were thawed according to the supplier’s instructions. Animal cells were seeded at 2000 (rhesus monkey) or 1000 cells/well (minipig, rat and mouse) and exposed as described earlier. Cell culture medium and all medium supplements were purchased from Sigma-Aldrich (Sweden) or Life Technologies (Sweden).
8
Cell Line Acquisition and Culture
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Male Fisher 344 rats were purchased from Japan SLC (Shizuoka, Japan). The rat aorta smooth muscle cell line, A7r5, was obtained from DS Pharma Biomedical Co. Ltd (Osaka, Japan). The human cervical cancer cell line, HeLa, and the human osteosarcoma cell line, HOS, were obtained from the Health Science Research Resources Bank (Osaka, Japan). Cell culture medium was purchased from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was purchased from JRH Biosciences (Lenexa, KS). Antibiotics were purchased from Life Technologies Japan Ltd. (Tokyo, Japan). Other reagents were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan), Sigma-Aldrich, and Life Technologies Japan Ltd.
9
Cell Culture Reagents and Protocols
10
Recombinant Cytokine-Stimulated Cell Culture
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rhM-CSF and rhRANKL were purchased from Peprotech (Hamburg, Germany). Chemicals, cell culture medium, and supplements were purchased from Sigma-Aldrich (Munich, Germany).
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