Data were acquired using the "Leica Qwin 500 C" image analyzer computer system Ltd. (Cambridge, England) in the Medical Histology and Cell Biology Department, Faculty of Medicine, Cairo University. The image analyzer included a colored video camera (Olympus), colored monitor, and hard disc of an IBM personal computer linked to the microscope and processed by the "Leica Qwin 500 C" software. The image analyzer was first calibrated to automatically convert the measurement units (pixels) produced by the image analyzer program into actual micrometer units. Slides were examined under the light microscope, and the following parameters were measured: a. Radial alveolar count in H&E-stained slides: measured in 200 × H&E-stained fields by drawing a perpendicular line from the center of a respiratory bronchiole to the nearest definitive alveolar septal wall [56] b. Area % of + ve CD31 immunoreactivity
Qwin 500 c
Manufactured by Leica
Sourced in United Kingdom
The Qwin 500 C is a laboratory equipment product designed for image analysis and measurement. It provides core functions for processing and analyzing digital images.
Automatically generated - may contain errors
18 protocols using qwin 500 c
1
Quantitative Histomorphometry of Alveolar Structure
2
Immunohistochemical Analysis of Apoptosis Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunohistochemical staining with rabbit polyclonal anti-Bax (ab53154), anti-Bcl2 (ab59348), and anti-caspase-3 (ab44976) primary antibodies (IHC-P, at 1:100 dilution, Abcam®, Cambridge, MA, United States) was performed via the Dako automated system (EnVision FLEX Peroxidase-blocked), as previously described (El Asar et al., 2019 (link)). In addition, the goat anti-rabbit IgG H&L (HRP) was added as the secondary antibody. Negative controls were obtained by omitting the primary antibodies in the automated staining protocol. The area percentage of the positive immune reaction of Bax, Bcl2, and caspase-3 was estimated using Leica Qwin 500C, Leica Image Analysis System Ltd. (Cambridge, England), in 10 live random fields (×400 magnification) from each group. The sections were evaluated using a Leica ICS150 HD microscope camera (Leica Imaging, Cambridge, United Kingdom).
3
Histological and Immunohistochemical Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After routine paraffin histological preparation, the LV specimen sections were subjected to staining with hematoxylin and eosin, and Masson Trichrome for evaluating tissue morphology and fibrosis and immunohistochemical evaluation of VEGF (1:50, rabbit polyclonal anti-VEGF primary antibody; Santa Cruz Biotechnology, Santa Cruz, California), CD31 (1:200, goat polyclonal anti-CD31 Ab; Abcam, Cambridge, Massachusetts), and caspase 3 (1:200, goat polyclonal anti-caspase 3 Ab; Abcam) as previously described. 26 Mouse spleen tissue served as a positive control. The negative controls were obtained in the automated staining protocol through omission of the primary antibody. All the histomorphometric parameters, including the number of foci of myocyte disarray, the percentage area of collagen fibers in Masson Trichrome-stained sections, the percentage area of VEGF, caspase 3 immunoreactivity. and the count of CD31 immunohistochemically positive blood capillaries were estimated via the Leica Imaging analyzer software system (Leica Qwin 500C; Leica, Cambridge, England) 27 with a standard measuring frame of 85 550 mm 28 in 10 randomly selected, nonoverlapping fields from each animal in the different study groups.
4
Histomorphometric Analysis of PAS and COX-2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Using ‘Leica Qwin 500 C’ image analyzer (Cambridge, UK), assessment of the area percent (%) of PAS positive reaction and COX-2 positive (+ve) immunostaining were accomplished. The measures were taken in 10 non overlapping high power fields (×400)/rat.
5
Hippocampal Neuronal and Glial Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Data were obtained using “Leica Qwin 500 C” image analyzer computer system Ltd. (Cambridge, England). Mean number of neurons (undamaged and dark neurons) / HPF in H&E stained sections and mean area percent of GFAP immunopositive cells were measured in DG of all immunostained hippocampal sections. From each section 10 non overlapping fields were examined using an objective lens ×40 (=total magnification ×400) and the mean value for each slide was obtained.
6
Quantifying Immunohistochemical Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Using the “Leica Qwin 500 C” image analyzer (Cambridge, United Kingdom), the area% of + ve PGR immunostaining in addition to the area% of + ve OSN at the mesometrial region, in 10 non-overlapping high-power fields (HPF) (×400)/rat were estimated.
7
Quantitative Lung Tissue Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the assessment of lung tissue, the following quantitative morphometric parameters were measured using Leica Qwin 500C” software image analyzer computer system (Cambridge, England), in control and experimental groups: the thickness of interalveolar septa in 10 non-overlapping high power fields of H&E stained sections (n = 10) at 400× magnification in all groups69 (link). In addition, the mean area percentage of the collagen fibers in sections stained with a Mallory’s trichrome stain was measured69 (link) and the mean percentage of PAS-positive goblet cells per bronchiole (number of PAS-positive goblet cells divided by the total epithelial cell number along the basement membrane of randomly selected transversely cut medium sized bronchiole having approximately 100–150 luminal airway epithelial cells) was determined on PAS stained slides for each group70 (link). These measurements were taken in 10 non-overlapping fields at 200 × magnification.
Finally, the mean count of alveolar macrophages in anti-CD68 immunostained sections67 (link) and the mean area percentage of positive iNOS immunoreactivity in anti-iNOS immunostained sections in 10 non-overlapping high power fields in all groups (n = 10) were assessed too68 (link). All these morphometrically measured data were statistically analyzed.
Finally, the mean count of alveolar macrophages in anti-CD68 immunostained sections67 (link) and the mean area percentage of positive iNOS immunoreactivity in anti-iNOS immunostained sections in 10 non-overlapping high power fields in all groups (n = 10) were assessed too68 (link). All these morphometrically measured data were statistically analyzed.
8
Quantifying Neuronal Pyknosis in Hippocampus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Data were obtained using (Leica Qwin 500 C) image analyzer computer system
Ltd. (Cambridge, England). For each group, five slides of six different
specimens were examined. Ten non-overlapping fields were measured in a
standard measuring frame for each slide. The mean number of pyknotic nuclei
of the neurons in the hippocampus was calculated using the interactive
measuring menu.
Ltd. (Cambridge, England). For each group, five slides of six different
specimens were examined. Ten non-overlapping fields were measured in a
standard measuring frame for each slide. The mean number of pyknotic nuclei
of the neurons in the hippocampus was calculated using the interactive
measuring menu.
9
Histological Assessment of Brain and Ovarian Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dissected brain and ovarian tissues were fixed in a 10% formalin-saline solution and processed into paraffin blocks. Next, 7-µm-thick sections were cut using a microtome and mounted on glass slides for hematoxylin and eosin (H&E) staining. Ovaries were sectioned horizontally and examined for inflammatory and cystic changes. Coronal sections of the brain were assessed for neuroinflammatory and neurodegenerative changes. As an index for neurodegeneration, the number of degenerated pyramidal cells was counted in layer 5 of the motor cortex. To do so, an Olympus light microscope (Japan) connected to a "Leica Qwin 500C" image analyzer system was used (Cambridge, UK) to examine the eight randomly selected high-power fields (× 400)/section in each group.
10
Quantifying Kidney CysC-Positive Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The area of CysC-positive immune staining in the sections from all kidney specimens was measured in all animals of the study. This was done in five non-overlapping fields at ×400 magnification in a field area of 7193.063 μm 2 for every section using Leica Qwin 500C image analyzer computer system (England) present in Histology Department, Faculty of Medicine, Cairo University. Images were captured live on the screen from sections under a light microscope (Olympus BX-40, Olympus Optical Co. Ltd., Japan) with affixed video camera (Panasonic Color CCTV camera, Matsushita Communication Industrial Co. Ltd., Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!